T-cell lymphomas of large cell type. A variety of malignant lymphomas: “Histiocytic” and mixed lymphocytic-“histiocytic” (original) (raw)
Related papers
Non-hodgkin's lymphomas:An ultrastructural study correlating morphology with immunologic cell type
Cancer, 1979
Ultrastructural studies were performed on 40 B-cell and 14 T-cell lymphomas of non-Hodgkin's type (NHL). Most B-cell lymphomas were comprised of neoplastic cells with morphologic features compatible with a follicular center cell origin. Dendritic reticulum cells and their desmosome-associated processes, characteristic of germinal centers, were observed in all 11 cases of nodular poorly differentiated lymphocytic lymphoma and in one of two cases of nodular "histiocytic" lymphoma, but were not identified in the lymphomas with a diffuse growth pattern. Desmosomes were observed between dendritic reticulum cells and were not found between lymphoid cells. Large neoplastic cells comprising lymphomas of "histiocytic," mixed lymphocytic "histiocytic," and "undifferentiated" types were characterized ultrastructurally and immunologically as lymphoid cells. Malignant lymphomas of well and moderately well differentiated lymphocytic types (7 cases) revealed B-cell markers, and represented a distinct homogenous group of neoplasms, with electron microscopic features most closely resembling follicular cuff lymphocytes. T-cell malignancies included lymphoblastic lymphomas (3 cases), large cell ("histiocytic") lymphomas (4 cases), lymphoepithelioid cell ("Lennert's") lymphomas (2 cases), mycosis fungoides (3 cases) and diffuse poorly differentiated lymphocytic lymphomas (2 cases). A consistent finding in the T-cell proliferations was the presence of small and/or large lymphoid cells with extremely irregular and/or convoluted nuclei, which occurred in varying proportions and with variable degrees of nuclear complexity. The nuclear irregularity evident in the neoplastic T cells was distinguishable from that observed for lymphoid cells of B-cell lymphomas. In comparing the cytoplasmic features of the Tand B-cell neoplasms ultrastructurally, the only distinguishing feature was the presence of well developed granular endoplasmic reticulum with dilated cisternae, i.s., plasmacytoid features, predictive of a B cell origin.
Virchows Archiv, 1984
A 16-yr-old white female was affected by continuous fever, pancytopenia with relative increase of T-8 lymphocytes, severe bone marrow hypoplasia, generalized lymphadenomegaly and splenomegaly. A first lymph hode biopsy, obtained at the onset of the disease, was involved by a paracortical tumor with some S-100 + "lymphocyte-like" cells in the neoplastic areas; in the cell suspension, 70-80 % of cells were E4 +/E37 + lymphocytes with prevalent expression of the T-8 phenotype (52 %). A second lymph hode biopsy, obtained five months later, was involved by a diffuse proliferation of S-100+ cells with high mitotic activity; in the cell suspension, the majority of cells were E-/T-11 + / T-3 +/T-8 +. At the TEM level, the neoplastic cells were characterized by regular or indented nuclei with finely dispersed chromatin and by regular or indented nuclei with finely dispersed chromatin and by irregular cytoplasmic profiles with thick pseudopodia-like projections. The possibility is discussed that this neoplasm may share some similarities with the T-7 lymphoma being part of a poorly described group of tumors with intermediate features between T cell lymphoma and malignant histiocytosis.
Cancer, 1981
Ten cases of T-lymphoblastic lymphoma/leukemia were studied with light and electron microscopy. Cytochernical stains were performed on touch preparations, and mononuclear cell suspensions were tested for spontaneous rosette formation with sheep erythrocytes, C3 receptors, and surface immunoglobulins. The present investigation was performed to evaluate several ultrastructural parameters, mainly the nuclear shape, as diagnostic clues for this group of lymphomas. Characteristic convoluted nuclei were present in 7 to 47% of the lymphoblasts. This percentage correlated with the focal acid phosphatase reaction and E-rosette formation. Acid phosphatase was the best cytochemical marker (70-10076 of the lymphoblasts showed focal reaction product). By ultrastructural cytochemistry, the reaction product was demonstrated in the Golgi cisternae and primary lysosomes. The cell suspensions obtained from different sources contained 14 to 95 % E-rosette-forming cells. No specific morphologic, cytochemical, or immunologic differences were found between patients with or without mediastinal involvement.
American Journal of Hematology, 1992
Two cases of lymphoma of large granular lymphocytes are reported. The first case expressed natural killer (NK) cell, some T-cell (CD 2, CD 5, CD a), and HLA-DR antigens, but was negative for other T-cell (CD 3, CD 4, CD 7), T-cell receptor (TCR), B-cell, and myeloid antigens. Germline configuration was demonstrated for TCR, and immunoglobulin heavy and light chain genes. The second case expressed NK cell, some T-cell (CD 3, CD 7, CD 8), and TCR antigens, but was negative for other T-cell (CD 4, CD 5), B-cell, myeloid, and HLA-DR antigens. Rearrangement of TCR u and p chains were detected. Thus, the findings of case 1 were consistent with true NK cell lineage and case 2 with NK-like T-cell lineage. Our report underscores the heterogeneity of this newly recognized lymphoma, which nevertheless carries a consistently poor prognosis and is probably more prevalent in the Asian population. This study also provides information concerning immunophenotypes of cellular infiltrates in internal organs and cytogenetic abnormalities in this lymphoma; neither has been reported frequently in the literature. The importance of detecting cytoplasmic granules in tissue imprints or electron micrographs for differentiating other T-cell lymphomas is emphasized, and the classification of large granular lymphoproliferative disorders is discussed.
journal of medical science and clinical research, 2017
Context: Diagnosis of lymphoma constitutes a difficult task in context to subtyping. Accurate diagnosis is essential because the treatment options, responses to therapy and prognosis vary widely depending on the diagnosis. However to make a definitive diagnosis of a lymphoma based solely on the H & E light microscopy, findings may be exceedingly difficult because of frequent absence of distinguishing features. IHC represents a tool that can provide a clear distinction among the different types of lymphoma. The purpose of IHC is to categorize the patient in order to ensure appropriate and specific treatment, as well as to identify tumours at higher risk of recurrence and fatal outcomes. Aims: To study histomorphology of different lymphomas and correlation of it with immunohistochemical findings. Settings and Design: The present study is a retrospective as well as prospective study of lymphoma cases diagnosed based on routine histopathology as well as correlation of it with immunohistochemistry in surgical pathology section of the Central Diagnostic Laboratory, Shree Krishna Hospital, Karamsad from September 2010 to August 2015. Materials and Methods: A detail clinical history, nature of specimen and other investigations were noted in the proforma. Macroscopic and microscopic findings or any incidental findings were documented in the final report. Results: Total 47 cases of lymphoma were diagnosed in study period among them 42 cases were of B cell lymphoma and 7 cases were of T cell lymphoma. All B cell lymphoma were positive immunihistochemically for CD 20 and LCA. Out of 47 cases 25(53.19%) were of lymphoid origin and 22(46.81%) were of extralymphoid site. Conclusion: From this study it was concluded that: In all the cases diagnosis of hematoxyllin and eosin stained sections were correlated with immunohistochemical findings Non Hodgkin's Lymphoma were most common than Hodgkin's lymphoma Lymphnodes are the most common site of origin.
Morphometric characterization of diffuse large-cell (histiocytic) lymphomas
The American journal of pathology, 1982
From 28 large-cell lymphomas, defined by marker studies, enzyme histochemistry, and electron microscopy as large noncleaved follicle center cell tumors (lnc FCC), B-immunoblastic or true histiocytic lymphomas, morphometric cell parameters, including nuclear size and shape, cytoplasmic area, and cytoplasm-to-nucleus ratio, were measured. Moreover, size, number, and location of nucleoli in the nuclei of the characteristic cells of lnc FCC and B-immunoblastic lymphoma were determined. Statistical evaluation of the data showed the nuclear shape to be the most sensitive parameter in the differentiation of true histiocytic lymphoma from lnc FCC and B-immunoblastic lymphomas. With nucleolar parameters lnc FCC could be differentiated from B-immunoblastic lymphoma: lnc FCC had a higher mean number of nucleoli per cross-section of nucleus and more eccentrically located nucleoli. Moreover, the measurements show that the differences in number and location of nucleoli between large noncleaved fo...
Characterization of two patients with lymphomas of large granular lymphocytes
Cancer, 1984
Two patients with non cutaneous well-differentiated lymphocytic lymphoma with leukemic spread are reported. The large majority of their peripheral b l d mononuclear cells (PBMC) formed rosetres wirh sheep erythrocytes, had receptors for the Fc portion of IgG, and an enzymaiic prufile uf relatively mature T-cells. These cells were morphologically characterized as large granular lymphocytes. Studies with monoclonal antibodies in one of the cases showed an OKT3f, OKTIO-, OKT4-, OKTB-, HNK -1 -, OKMI + phenotype, whereas PBMC from the other case were OKT3t, OKTIO-, OKT4-, OKT8+, H N K -l t , OKM1-. PBMC from the first patient were able to suppress in virro B-cell differentiation and were capable of a strong antibody dependent cellular cytotouiciiy I ADCC) activity. Natural killer (NK) activity was reduced. Cells from the other patient who was hypngammaglobulincrnic, everted suppressor activity in immunoregulatory assays, and showed A M C and NK activity. 'I'hese data support the existence of I,GL lymphomas consisting of the proliferation cri mature appearing cells capable of functional activity. the~r generous: aft of monoclonal antibodies and also Ms. A. H. Constantme for editorial assistance.
Hematological Oncology, 2006
Forty-five bone marrow (BM) biopsies have been studied in 30 T-cell malignant lymphoma (ML). According to the updated Kiel classification, these ML comprised 12 low grade ML and 18 high grade ML. BM involvement was not significantly more frequent in low grade ML (41.6 per cent) than in high grade (33-3 per cent). This involvement was discovered in 85 per cent of the cases at the time of diagnosis. A correlation was found between BM and other visceral localizations for histological type in all cases. Infiltrates principally showed a nodular pattern in low grade and a diffuse pattern in high grade ML. Hematopoietic hyperplastic reaction was frequent (66.6 per cent) not correlated to involvement. Clinical staging showed extensive spreading. Our patients had an overall median survival of 40 months, worse in high grade ML (median: 19 months) than in low grade ML (41 months) but not statistically different @= 0.25). Extranodal localizations are a significant criteria for poor prognosis (p = 0.018). Among them, BM involvement appears to be the most significant criteria (Cox model, p = 0.006). Patients with BM localization had a median survival of 9 months contrasting with 40 months in patients without BM localization (p =0.007).
Leukemia Research, 1990
Abstraet-A group of 12 large-cell lymphomas, seen in the period January 1983 to January 1985 and then tentatively classified as histiocytic sarcomas/true histiocytic lymphomas (i.e. non B-, non T-cell lymphomas, with expression of some histiocytic markers) were restudied phenotypically with a much more extensive range of monoclonal and polyclonal antibodies. They were also genotypically analyzed with respect to rearrangements of genes coding for the immunoglobulin (Ig) heavy and light chains and the gamma and beta chains of the T-cell receptor (TCR). The combined results suggest that these "histiocytic sarcomas" represent a heterogeneous group of large cell lymphomas often with unexpected rearrangements (or lack of rearrangements) of either Ig and TCR genes and phenotypically with coexpression of "histiocytic" and B-or T-cell markers. Such coexpression may be regarded as a warning signal to expect unusual genotype. True histiocytic tumours apparently do exist, but are rare (one case in this study), and their diagnosis is very difficult.
Histopathology, 1987
Band T-cell non-Hodgkin's lymphomas with large multilobated cells: morphological, phenotypic and clinical heterogeneity Ten cases of non-Hodgkin's lymphomas, mainly composed of large multilobated cells, have been studied. Our results are consistent with the view that they represent a somewhat heterogeneous group of lymphoid tumours displaying different morphological, clinical and immunophenotypic features. In B-cell type the large multilobated cells were histologically characterized by prominent nucleoli and distinctly basophilic cytoplasm whereas in the T-cell type they had indistinct or small nucleoli and ill-defined weakly eosinophilic cytoplasm. These differential features between Band T-cell type were confirmed by electron microscopy. From a clinical standpoint B-cell type was characterized by a constant involvement of lymphoid tissues (lymph nodes and/or Waldeyer's ring); T-cell type showed, on the contrary, a more frequent involvement of extra-lymphoid sites (mainly bone and subcutaneous tissues). Our study provides some morphological features that may be helpful for a correct differential diagnosis in this heterogeneous group of non-Hodgkin's lymphomas.