HPLC analysis of flavonoids in Eupatorium littorale (original) (raw)
Related papers
2013
A rapid RP-HPTLC densitometry method for simultaneous determination of major flavonoids in important medicinal plants A simple, sensitive, selective, precise, and robust high-performance TLC (HPTLC) method was developed and validated for determination of flavonoids in herbal extracts Bauhinia variegata, Bacopa monnieri, Centella asiatica, Ginkgo biloba, Lonicera japonica, Rosa bourboniana, Rosa brunonii, and Rosa damascena. The HPTLC of flavonoids was performed on RP-18 F 254 TLC plates with dual run, water (5% formic acid)/methanol (70:30) and water (5% formic acid)/methanol (50:50) as mobile phases. Densitometric determination of flavonoids was performed at k = 280 nm in reflectance/ absorbance mode. The linear regression analysis data for the calibration plots showed a good linear relationship with r 2 = 0.998 l 0.0003 in the concentration range of 150 -800 ng/spot for apigenin and rutin and 200 -1000 ng/spot for quercetin, luteolin, and quercitrin with respect to peak area. The average recovery for apigenin, quercetin, rutin, luteolin, and quercitrin was 97 -99.8% indicating the excellent reproducibility. Statistical analysis of the data showed that the method is reproducible and selective for determination of flavonoids.
Identification and assay of the flavonoids in medicinal plants with hepatoprotective action
Modern Phytomorphology, 2015
The article describes the identification and assay of the flavonoids in medicinal plants with hepatoprotective action, harvested as a culture at the Cultivation Center of the Medicinal Plants within State University of Medicine and Pharmacy „Nicolae Testemițanu” from the Republic of Moldova, using Pharmacopoeia methods. The flavonoids, found in the examined medicinal product, are responsible for hepatoprotective activity due to antioxidant activity, exhibited by neutralizing free radicals. The flavonoids were identified by using the Chinode method and thin layer chromatography, operating with 3 systems of solvents, and as biomarkers rutine, quercetine and luteolin ewere used. The results of the quantitative analysis point out that the content of the flavonoids determined by using spectrophotometric method is in the range of 0.620% to 1.204%, in studied vegetal products.
Determination Of The Presence Of Flavonoids In The Leaves, Seeds And Branches Of The Matured
2015
Abstract: The extracts were concentrated to obtain a mass of 0.9g, 1.2g, 1.4g of seeds, leaves and branches respectively. After the separation of the pigment into hexane, the mass of the residue obtained were 0.7g for seeds, 0.9g for leaves and 1.0g for branches. The Cyanidin’s test for the various masses of the extracts of the plant materials after washing with hexane gave the evidence of the presence of flavonoids. The number of components could not be obtained from the eluates of the column chromatography, because there were no T.L.C materials available. But when the component of each plant material was subjected to cyanidin’s test, it gave the proof of the presence of flavonoid.
Flavonoid Content of Eupatorium glandulosum and Coolebroke oppositifolia
2001
Objective: Estimation of quercetin content of Eupatorium glandulosum and Coolebroke oppositifolia by HPLC. Materials and methods: The presence of quercetin (3, 3 I , 4 I , 5, 7 - pentahydroxyflavone) present in the leaves of Eupatorium glandulosum (Family- Asteraceae) and Coolebroke oppositifolia (Family : Labiatae) was quantitatively estimated by high performance liquid chromatography using reversed phase (RP-HPLC) with stepwise gradient elution on a RP C18 column. A two step elution with: acetonitrile- water- phosphoric acid (85%): from 16-83-1 to 32-67-1(v/v) was made. Results: The leaf extracts of E. glandulosum and C. oppositifolia contained 4.96 and 2.10 % and the respective powdered leaf contained 0.19 % (w/w) and 0.09 % (w/w) of quercetin . Conclusion: The method developed is useful to obtain quantitative values for quercetin present in these plant species, which may be useful for standardization of the same.
Isolation and Characterization of Flavonols by HPLC-UV-ESI-MS/MS from <i>Talipariti elatum</i> S.w
American Journal of Plant Sciences, 2016
The red petals of the flowers of Talipariti elatum, commonly named majagua is used as antitussive, expectorant and antasthmatic in phytotherapy, although the plants' composition has not been determined in detail so far. Hence, in this study, we present a validated, sensitive, reliable, and cheap narrow-bore LC-UV-ESI-MS/MS coupled to PDA (photodiode array detectors) method for the simultaneous isolation and identification of flavonoids and their glycosidic derivatives in this flower drug. In addition, the structures of two compounds have been elucidated by LC-MS experiments after isolation. Structure analyses allow proposing the presence of gossypitrin (gossypetin-7-Oglucoside) or gossypetin-3'-O-glucoside, a quercetin derivative, possibly quercetin-3-O-glucoside and an unidentified compound with an impair number of m/z, probably an alkaloid.
" Qualitative and Quantitative Analysis of Flavonoids "
Flavonoids and Polyphenolic compounds have been given sizeable attention in this review; distinctively for the reason of their biological and physiological importance. This review emphasis on separation and revealing qualitative and quantitative estimation methods of flavonoids. This literature inspects various techniques used for quantitative and qualitative estimation of flavonoids. These techniques embrace, for example, HPLC, ultraviolet-visible spectroscopy, mass spectrometry, nuclear magnetic resonance spectroscopy. The inclusive topics are structural characterization by different techniques, conduct of sample with different solvents of different polarity, extraction, chromatography techniques such as liquid chromatography (LC), and gas chromatography (GC), electrophoresis. Emphasis will be on up to date developments and leaning.
Isolation and Characterization of Flavonols by HPLC-UV-ESI-MS/MS from Talipariti elatum S.w
American Journal of Plant Sciences, 2016
The red petals of the flowers of Talipariti elatum, commonly named majagua is used as antitussive, expectorant and antasthmatic in phytotherapy, although the plants' composition has not been determined in detail so far. Hence, in this study, we present a validated, sensitive, reliable, and cheap narrow-bore LC-UV-ESI-MS/MS coupled to PDA (photodiode array detectors) method for the simultaneous isolation and identification of flavonoids and their glycosidic derivatives in this flower drug. In addition, the structures of two compounds have been elucidated by LC-MS experiments after isolation. Structure analyses allow proposing the presence of gossypitrin (gossypetin-7-Oglucoside) or gossypetin-3'-O-glucoside, a quercetin derivative, possibly quercetin-3-O-glucoside and an unidentified compound with an impair number of m/z, probably an alkaloid.
Pharmacognosy Research
Background: The traditional use of Eugenia uniflora L. ("Pitanga") is reported due to several properties, which have often been related to its flavonoid content. Objective: The aim was to evaluate analytical procedures for quantification of total flavonoids content (TFCs) by ultraviolet-visible (UV-Vis) spectrophotometry in the herbal material (HM), crude extract (CE), and fractions from leaves of E. uniflora. Materials and Methods: The method for quantification of flavonoids after complexation with aluminum chloride (AlCl 3) was evaluated: amount of sample (0.25-1.5 g); solvent (40%-80% ethanol); reaction time and AlCl 3 concentration (2.5%-7.5%). The procedures by direct dilution (DD) and after acid hydrolysis (AH) were used and validated for HM and CE and applied to the aqueous fraction (AqF), hexane fraction, and ethyl acetate fractions (EAF). Results: The ideal conditions of analysis were ethanol 80% as solvent; 0.5 g of sample; λmax of 408 (DD) and 425 nm (AH); 25 min after addition of AlCl 3 5%. The procedures validated for standards and samples showed linearity (R² > 0.99) with limit of detection and limit of quantification between 0.01 and 0.17 mg/mL (rutin and quercetin); and 0.03 and 0.09 mg/mL (quercetin), for DD and AH, respectively. The procedures were accurate (detect, practice, and repair <5% and recovery >90%), and stable under robustness conditions (luminosity, storage, reagents, and equipment). The TFCs in AqF and EAF were 0.65 g% and 17.72 g%, calculated as rutin. Conclusions: UV-Vis methods for quantification of TFC in HM, CE, and fractions from leaves of E. uniflora were suitably validated. Regarding the analysis of fractions, the EAF achieved enrichment of about nine times in the content of flavonoids.