Synthesis and study of nucleic acids interactions of novel monomethine cyanine dyes (original) (raw)

Dyes and Pigments, 2012

Abstract

ABSTRACT Six asymmetric monomethine cyanine dyes have been synthesized and their spectral characteristics and interaction with double stranded (ds)DNA have been investigated for their prospective use as fluorescent markers in molecular biology. Therefore, the non-covalent binding of the compounds with dsDNA was explored. Apart from the fluorescence spectroscopy, the study includes UV/Vis spectrophotometry and circular dichroism spectroscopy, as well as the thermal melting experiments. Although the compounds show relatively low binding affinitiy towards dsDNA, do not have intrinsic fluorescence, however in the presence of dsDNA they exhibited enhancement in fluorescence intensity. Therefore the studied dyes show interesting platform for future modifications directed towards more sequence selective derivatives. The compound with the highest affinity towards dsDNA showed interesting anti proliferative potential and specificity

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