Similarities in the Architecture of the Active Sites of Ni-Hydrogenases and Fe-Hydrogenases Detected by Means of Infrared Spectroscopy (original) (raw)

This study investigates the similarities in the architecture of the active sites of Ni-hydrogenases and Fe-hydrogenases through infrared spectroscopy. The results indicate the potential existence of a bi-metallic center, composed of Ni/Fe in Ni-hydrogenases and Fe/Fe in Fe-hydrogenases, which includes low-spin Fe ions and thiols from cysteine residues. Key spectral bands suggest structural homology between the active sites of these two hydrogenase families, despite previously assumed differences.