Plasma constituent integrity in pre-storage vs. post-storage riboflavin and UV-light treatment – A comparative study (original) (raw)
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Background/Aim. After the introduction of a careful selection procedure for blood donors and the implementation of highly sensitive screening tests for transfusion-transmitted infections (TTIs), blood has become a very safe product concerning TTIs. However, due to the existence of a ?window? period during which these ?markers? cannot be detected, as well as the emergence of new pathogens, the risk is still present. Implementation of pathogen reduction technology (PRT) provides a proactive approach to improving blood safety. By damaging nucleic acids, PRT selectively inactivates pathogens and leucocytes. Nevertheless, during the process, plasma proteins are also damaged to some extent. The aim of this study was to conclude whether there is a difference in the effect of PRT on protein S (PS) and alpha 2-antiplasmin (?2AP) regarding the time of inactivation: inactivation immediately after plasma separation from whole blood (before freezing) vs. inactivation after freezing/thawing. Meth...
2021
Background/Aim. After introduction of carefull selection procedure for blood donors and the implemention of highly sensitive screening tests for transfusion transmissible infections (TTI), blood is very save product concerning TTI. Nevertheless, because of the ?window? period for the pathogen that are testing and the emergence of new pathogens, the risk stil persists. Implementation of pathogen reduction technology (PRT) provides a proactive approach to improve blood safety. By damaging nucleic acids, PRT selectively inactivates pathogens and leucocytes. However, during the process, plasma proteins are, also, damaged in some extent. The goal of this study is to conclude if there is the difference in the effect of PRT on Protein S and ?2-antiplasmin regarding the time of inactivation: inactivation immediately after plasma separation from whole blood (before freezing) versus inactivation after freezing/thawing. Methods. The voluntary donors' blood is taken into quadruple bag syste...
Transfusion, 2013
BACKGROUND: According to AABB standards, freshfrozen plasma (FFP) should be thawed at 30 to 37°C and expire after 24 hours. An increase in the aggressive management of trauma patients with thawed plasma has heightened the risk of plasma waste. One way to reduce plasma waste is to extend its shelf life, given that the full range of therapeutic efficacy is maintained. We evaluated the effect of prolonged storage at 1 to 6°C on the activity of Factor (F)V, FVII, and FVIII in plasma thawed at 37 or 45°C. STUDY DESIGN AND METHODS: Group O plasma from healthy donors (n = 20) was divided into 10 pairs and frozen and stored at not more than -18°C. One sample from each pair was thawed at 37 or 45°C, and all were stored at 1 to 6°C. Samples were analyzed for FV, FVII, and FVIII activity on Days 0, 5, 10, 15, and 20. RESULTS: Plasma thawing time was 17% less at 45°C than at 37°C. No differences were observed between thawing groups in coagulation activity of FV, FVII, and FVIII during the 20-day storage period (p > 0.12). In both groups, the activity of FV and FVIII decreased over time but remained within a normal range at 10 days. CONCLUSION: Although levels of plasma clotting factors are reduced in storage, therapeutic levels of FV and FVIII are maintained in thawed plasma stored for up to 10 days at 1 to 6°C. Thawing of FFP at 45°C decreases thawing time but does not affect the activity of FV, FVII, and FVIII. * Data are presented as the mean percent activity Ϯ SD. Reference ranges: FV, 34-108; FVII, 28-104; and FVIII, 50-178.
The effects of frozen tissue storage conditions on the integrity of RNA and protein.
2014
Unfixed tissue specimens most frequently are stored for long term research uses at either −80° C or in vapor phase liquid nitrogen (VPLN). There is little information concerning the effects such long term storage on tissue RNA or protein available for extraction. Aliquots of 49 specimens were stored for 5–12 years at −80° C or in VPLN. Twelve additional paired specimens were stored for 1 year under identical conditions. RNA was isolated from all tissues and assessed for RNA yield, total RNA integrity and mRNA integrity. Protein stability was analyzed by surface-enhanced or matrix-assisted laser desorprion ionization time of flight mass spectrometry (SELDI-TOF-MS, MALDI-TOF-MS) and nano-liquid chromatography electrospray ionization tandem mass spectrometry (nLC-ESI-MS/MS). RNA yield and total RNA integrity showed significantly better results for −80° C storage compared to VPLN storage; the transcripts that were preferentially degraded during VPLN storage were these involved in antigen presentation and processing. No consistent differences were found in the SELDI-TOF-MS, MALDI-TOF-MS or nLC-ESI-MS/MS analyses of specimens stored for more than 8 years at −80° C compared to those stored in VPLN. Long term storage of human research tissues at −80° C provides at least the same quality of RNA and protein as storage in VPLN.
Appropriate and Inappropriate Use of Fresh Frozen Plasma (FFP) and Packed Cell Volume (PCV)
International Journal of Health Sciences and Research, 2014
The aim of this study was to evaluate the usage of fresh frozen plasma (FFP) and packed cell volume (PCV) according to indications and to reduce inappropriate usage. Method: A two years retrospective study was conducted in Dr. S.C.G.M.C. and hospital blood bank. Based on the guidelines published by college of American pathologist, national health and medical research council and Australian society for blood transfusion FFP and PCV usage were categorized into appropriate and inappropriate. Pre and post transfusion INR/PT were recorded and the effect of FFP were studied in patients who received FFP Results: During two years study 1079 unit of FFP were used for 267 patients. Out of 267 patients only 125(46.81%) request were appropriate and 142(53.19%) were inappropriate requests. Pregnant female with active labour suffering from severe anaemia with shock was the commonest reason for inappropriate FFP use. Out of 125 appropriate request 100 patients were compared by evaluating Pre and post transfusion PT/INR by using fully automated coagulometer. Total 993 PCV units were transfused in 445 patients out of which 358 were appropriate and 87 were inappropriate as per the guidelines. Highest appropriate request were from pediatric department followed by gynaecology department. Conclusion: Inappropriate use of FFP and PCV not only increase the treatment costs, but also causes loss of productive power and exposes the patient to the unnecessary side effects of transfusion. Inappropriate FFP and PCV transfusion should be prevented by means of education and awareness programme by establishing the hospital transfusion guidelines
Effect of overnight 4oC storage of whole blood on von Willebrand factor
Transfusion, 2006
Cardigan and colleagues 1 recently reported their experience with overnight 4°C ("cold-temperature") storage of whole blood, and the hemostatic quality of fresh-frozen plasma (FFP) subsequently generated from this material. They found some loss of fibrinogen, Factor (F)V, FVIII, and FXI (12, 15, 23, and 7%, respectively; compared with historical data on FFP generated with material stored for < 8 hr), but no significant loss of any other factor, including von Willebrand factor (VWF). Their findings raise a number of important questions and appear to be at odds with previously reported data. 2-7 Our laboratory, for example, has reported several times 2-4 that storage of citrate anticoagulated whole blood at 4°C leads to significant cold induced loss of FVIII activity and VWF activity and antigen. These studies, from the context of laboratory hemostasis diagnostics, indicated a significant potential for misdiagnosis of mild hemophilia A, or of von Willebrand disorder (or disease; VWD), in a subset of individuals when samples were stored in that manner. These findings have since been confirmed and extended by two recent independent studies. 5,6 The study of Cardigan and colleagues 1 also appears to be at odds with an earlier blood bank study, indicating loss of VWF with coldtemperature storage unless samples were leukodepleted. 7 So, how does one resolve these study discrepancies? Does cold-temperature storage of whole blood lead to a significant loss of VWF? In part, study discrepancies might have arisen simply from the differential approaches undertaken. In the studies assessing loss of VWF with respect to laboratory diagnostics, 2-6 samples tested from after cold storage of whole blood were directly compared to aliquots from the same samples taken before this storage. In contrast, Cardigan and colleagues 1 undertook indirect comparisons (samples tested after overnight cold storage of whole blood were compared with historical data with a similar number of samples taken from standard procedure-generated FFP). To assess whether this might account for the discrepancy, data from our laboratory have been reanalyzed as shown in . After cold storage of whole blood, data are still significantly lower than data generated with more ideal (standard) processing procedures, suggesting that this is therefore unlikely to account for study discrepancies.