Phytocompounds and modulatory effects of Anacardium microcarpum (cajui) on antibiotic drugs used in clinical infections (original) (raw)

Abstract

Background: The challenge of antibiotic resistance and the emergence of new infections have generated considerable interest in the exploration of natural products from plant origins as combination therapy. In this context, crude ethanolic extract (CEE), ethyl acetate fraction (EAF), and methanolic fraction (MF) from Anacardium microcarpum were tested alone or in combination with antibiotics (amikacin, gentamicin, ciprofloxacin, and imipenem) against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Methods: Antibiotic resistance-modifying activity was performed using the microdilution method by determining the minimal inhibitory concentration (MIC). In addition, phytochemical prospecting analyses of tested samples were carried out. Results: Our results indicated that all the extracts showed low antibacterial activity against multidrug-resistant strains (MIC =512 μg/mL). However, addition of CEE, EAF, and MF to the growth medium at the subinhibitory concentration (MIC/8=64 μg/mL) significantly modulated amikacin- and gentamicin-resistant E. coli 06. CEE and EAF also demonstrated a significant (P,0.001) synergism with imipenem against S. aureus. In contrast, MF antagonized the antibacterial effect of ciprofloxacin and gentamicin against P. aeruginosa 03 and S. aureus 10, respectively. Qualitative phytochemical analysis of the extracts revealed the presence of secondary metabolites including phenols, flavonoids, xanthones, chalcones, and tannin pyrogallates. Conclusion: Taken together, our results suggest that A. microcarpum is a natural resource with resistance-modifying antibacterial activity that needs to be further investigated to overcome the present resistant-infection problem.

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References (40)

  1. Rapp RP. Changing strategies for the management of invasive fungal infections. Pharmacotherapy. 2004;24:4S-28S.
  2. Tenover FC. Mechanisms of antimicrobial resistance in bacteria. Am J Med. 2006;119:S3-S10.
  3. Gullo A. Invasive fungal infections: the challenge continues. Drugs. 2009; 69 Suppl 1:65-73.
  4. World Health Organization. Traditional Medicine -Growing Needs and Potential. WHO Policy Perspectives on Medicine. No 2. Geneva: World Health Organization; 2002. Available from: http://apps.who.int/ medicinedocs/en/d/Js2293e/
  5. Fleckenstein JM, Hardwidge PR, Munson GP, Rasko DA, Sommerfelt H, Steinsland H. Molecular mechanisms of enterotoxigenic Escherichia coli infection. Microbes Infect. 2010;12:89-98.
  6. Martin SJ, Yost RJ. Infectious diseases in the critically ill patients. J Pharm Pract. 2011;24:35-43.
  7. Lavoie EG, Wangdi T, Kazmierczak BI. Innate immune responses to Pseudomonas aeruginosa infection. Microbes Infect. 2011;13:1133-1145.
  8. Hidron AI, Edwards JR, Patel J, et al; National Healthcare Safety Network Team; Participating National Healthcare Safety Network Facilities. NHSN annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006-2007. Infect Control Hosp Epidemiol. 2008;29:996-1011.
  9. Bibi Y, Nisa S, Chaudhary F, Zia M. Antibacterial activity of some selected medicinal plants of Pakistan. BMC Complement Altern Med. 2011;11:52.
  10. Lima AF, Costa LB, Silva JL, Maia MB, Ximenes EC. Interventions for wound healing among diabetic patients infected with Staphylococcus aureus: a systematic review. Sao Paulo Med J. 2011;129:165-170.
  11. Colonna C, Dorati R, Conti B, Caliceti P, Genta I. Sub-unit vaccine against S. aureus-mediated infections: set-up of nano-sized polymeric adjuvant. Int J Pharm. 2013;452:390-401.
  12. Tripathi KD. Antimicrobial drugs. In: Essentials of Medicinal Pharmacol- ogy. Jaypee Brothers Medical Publishers, New Delhi; 2008:667-818.
  13. Sahu MC, Dubey D, Rath S, Debata NK, Padhy RN. Multidrug resis- tance of Pseudomonas aeruginosa as known from surveillance of nosocomial and community infections in an Indian teaching hospital. Journal of Public Health. 2012;20:413-423.
  14. Kumar K, Chopra S. New drugs for methicillin-resistant Staphylococcus aureus: an update. J Antimicrob Chemother. 2013;68:1465-1470.
  15. Tran TD, Do TH, Tran NC, et al. Synthesis and anti methicillin resistant Staphylococcus aureus activity of substituted chalcones alone and in combination with non-beta-lactam antibiotics. Bioorg Med Chem Lett. 2012;22:4555-4560.
  16. Matias EF, Santos KA, Almeida TS, Costa JG, Coutinho HD. Phy- tochemical prospection and modulation of aminoglycoside antibiotic activity by Croton campestris A. Chemotherapy. 2011;57:305-309.
  17. Sánchez-Medina A, García-Sosa K, May-Pat F, Peña-Rodríguez LM. Evaluation of biological activity of crude extracts from plants used in Yucatecan traditional medicine part I. Antioxidant, antimicrobial and beta- glucosidase inhibition activities. Phytomedicine. 2001;8:144-151.
  18. Weckesser S, Engel K, Simon-Haarhaus B, Wittmer A, Pelz K, Schempp CM. Screening of plant extracts for antimicrobial activity against bacteria and yeasts with dermatological relevance. Phytomedicine. 2007;14:508-516.
  19. de Almeida TS, Rocha JB, Rodrigues FF, Campos AR, da Costa JG. Chemical composition, antibacterial and antibiotic modulatory effect of Croton campestris essential oils. Ind Crops Prod. 2013;44:630-633.
  20. Barreto HM, Silva Filho EC, Lima EO, et al. Chemical composition and possible use as adjuvant of the antibiotic therapy of the essential oil of Rosmarinus officinalis L. Ind Crops Prod. 2014;59:290-294.
  21. Dubey D, Sahu MC, Rath S, Paty DP, Debata NK, Padhy RN. Anti- bacterial activity of medicinal plants used by aborigines of Kalahandi, Orissa, India against multidrug resistant bacteria. Asian Pac J Trop Biomed. 2012;2:S846-S854.
  22. Dubey D, Padhy RN. Surveillance of multidrug resistance of two Gram- positive pathogenic bacteria in a teaching hospital and in vitro efficacy of 30 ethnomedicinal plants used by an aborigine of India. Asian Pac J Trop Dis. 2012;2:273-281.
  23. Arokiyaraj S, Sripriya N, Bhagya R, Radhika B, Prameela L, Udayaprakash NK. Phytochemical screening, antibacterial and free radical scavenging effects of Artemisia nilagirica, Mimosa pudica and Clerodendrum siphonanthus -an in-vitro study. Asian Pac J Trop Biomed. 2012;2:S601-S604.
  24. Tajkarimi MM, Ibrahim SA, Cliver DO. Antimicrobial herb and spice compounds in food. Food Control. 2010;21:1199-1218.
  25. Gyawali R, Ibrahim SA. Impact of plant derivatives on the growth of foodborne pathogens and the functionality of probiotics. Appl Microbiol Biotechnol. 2012;95:29-45.
  26. Gyawali R, Adkins A, Minor RC, Ibrahim SA. Behavior and changes in cell morphology of Escherichia coli O157:H7 in liquid medium and skim milk in the presence of caffeine. CyTA: Journal of Food. 2014; 12:235-241.
  27. Barbosa Filho VM, Waczuk EP, Kamdem JP, et al. Phytochemical constituents, antioxidant activity, cytotoxicity and osmotic fragility effects of caju (Anacardium microcarpum). Ind Crops Prod. 2014;55: 280-288.
  28. Matos FJ. Introdução à Fitoquímica Experimental. Fortaleza: Edições UFC; 1997. Portuguese.
  29. National Committee for Clinical Laboratory Standards (NCCLS). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. 6th ed. NCCLS approved standard M7-A5, v 20, n 2. Villanova: NCCLS; 2003.
  30. Javadpour MM, Juban MM, Lo WC, et al. De novo antimicrobial peptides with low mammalian cell toxicity. J Med Chem. 1996;39: 3107-3113.
  31. Coutinho HD, Costa JG, Lima EO, Falcão-Silva VS, Siqueira-Júnior JP. Enhancement of the antibiotic activity against a multiresistant Escheri- chia coli by Mentha arvensis L. and chlorpromazine. Chemotherapy. 2008;54:328-330.
  32. Dall'Agnol R, Ferraz A, Bernardi AP, et al. Antimicrobial activity of some Hypericum species. Phytomedicine. 2003;10:511-516.
  33. Tanaka JC, da Silva CC, Filho BP, Nakamura CV, de Carvalho JE, Foglio MA. Chemical constituents of Luehea divaricata Mart. (Tiliaceae). Quim Nova. 2005;28:834-837. Portuguese.
  34. Chen LF, Kaye D. Current use for old antibacterial agents: polymyx- ins, rifamycins, and aminoglycosides. Med Clin North Am. 2011;95: 819-842.
  35. Tsuchiya H, Sato M, Miyazaki T, et al. Comparative study on the antibacterial activity of phytochemical flavanones against methicillin- resistant Staphylococcus aureus. J Ethnopharmacol. 1996;50:27-34.
  36. Juven BJ, Kanner J, Schved F, Weisslowicz H. Factors that interact with the antibacterial action of thyme essential oil and its active constituents. J Appl Bacteriol. 1994;76:626-631.
  37. Rodrigues FF, Costa JG, Coutinho HD. Synergy effects of the antibiotics gentamicin and the essential oil of Croton zehntneri. Phytomedicine. 2009;16:1052-1055.
  38. Veras HN, dos Santos IJ, dos Santos AC, et al. Comparative evalu- ation of antibiotic and antibiotic modifying activity of quercetin and isoquercetin in vitro. Curr Top Nutraceutical Res. 2011;9:25-30.
  39. Behling EB, Sendão MC, Francescato HD, Antunes LM, Bianchi ML. Flavonoid quercetin: general aspects and biological actions. Alimentos e Nutricao. 2004;15:285-292.
  40. Granowitz EV, Brown RB. Antibiotic adverse reactions and drug interactions. Crit Care Clin. 2008;24:421-442.