Secondary metabolites inin vitro cultured plants of the genusDrosera (original) (raw)
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2014
© 2014, Galvina M. Ferreira, Institute of Chemical Technology 5. RESULTS AND DISCUSSION Quinones are biologically active compounds which are found to occur in number of medicinal plants. They are classified into benzoquinones, naphthoquinones, anthraquinones, phenanthraquinones, condensed quinones and anthracyclinones. The objective of this research work is to present the methods for separation of important naphthoquinones and benzoquinones from medicinal plants and subsequent chemical modification for preparation of newer derivatives from the same. With this objective work was undertaken for extraction and isolation of Embelin, Thymoquinone, Plumbain and Shikalkin from Embelia ribes, Nigella sativa, Plumbago zeylanica and Arnebia nobilis respectively. The crude drugs were obtained from local market in Mumbai and authenticated, dried powdered and used for extraction.
Central European Journal of Chemistry, 2011
Rapid Resolution HPLC/DAD method, on a 1.8 µm, 4.6×50 mm column, was developed to enable a rapid separation of a mixture of 17 compounds, which consisted of hydroxybenzoic acids, hydroxycinnamic acids, flavones, flavonols, flavanone, flavonol-glycoside and antraquinone, in a single run, within 22 minutes. The developed method is precise, accurate and sensitive enough for simultaneous quantitative evaluation of major compounds
Analytical and Bioanalytical Chemistry, 2011
Nine flavonol glycosides (quercetin-3-O-glucuronide, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, kaemperol-3-Oglucuronide, kaemperol-3-O-rutinoside, kaempherol-3-O-glucoside, isorhamnetin-3-O-glucuronide, isorhamnetin-3-Orutinoside and isorhamnetin-3-O-glucoside) were isolated from the aerial parts of Chuquiraga spinosa (R. et P.) D. Don (Asteraceae). The identification of the compounds was carried out by HPLC/DAD, HPLC/MS and NMR analysis. These compounds may be useful in the chemotaxonomy of the genus and species.
Quantification of Flavonoids, Naphthopyranones and Xanthones in Eriocaulaceae Species by LC-PDA
American Journal of Analytical Chemistry, 2012
The linearity, stability, accuracy and inter-day precisions of the assay method were evaluated in methanolic aerial-part extracts of Paepalanthus giganteus and Syngontnhus nitens from the Eriocaulaceae family. Their small capitulae hinder morphological analysis, and thus complicate taxonomic studies of these species, which present anti-ulcer, antimutagenic and antioxidant activities. Taxonomic studies of these plants revealed that the Paepalanthus genus presents flavonols and naphthopyranones while the Syngontnhus genus has flavone and xanthone as majority compounds. The prepared samples were analyzed quantitatively by High Performance Liquid Chromatography with PDA detection for the presence of quercetin, luteolin, 3,6-dimethoxy-1,5,7-tri-hydroxyxanthone and paepalantine. The substances were recovered from these samples at rates from 98.01% to 99.99%. The coefficient of variation in the quantitative analysis of the sample compounds was under 5%. The linearity of the method was determined by linear regression. The analysis of the samples spiked with known amounts of analyte demonstrated that the response was proportional to the concentrations of the samples with respective determination coefficients of r 2 = 0.9999 (luteolin and 3,6-dimethoxy-1,5,7-tri-hydroxyxanthone) and r 2 = 0.9998 (quercetin and paepalantine) for the linear range of the analytical calibration curves of the samples. The detection limits were 0.07 µg•mL-1 for quercetin and luteolin, 0.06 µg•mL-1 for 3,6-dimethoxy-1,5,7-tri-hydroxyxanthone and 0.10 µg•mL-1 for paepalantine. The quantification limits were 0.23 µg•mL-1 for quercetin and luteolin, 0.20 µg•mL-1 for 3,6-dimethoxy-1,5,7-tri-hydroxyxanthone and 0.33 µg•mL-1 for paepalantine by LC. The method was considered sensitive for quantification of the quercetin, luteolin, 3,6-dimethoxy-1,5,7-tri-hydroxyxanthone and paepalantine in plant samples.
International Journal of Analytical Chemistry, 2015
Quantification of the four flavonoids, namely, luteolin, kaempferol, diosmetin, and chrysosplenetin, has been performed for the first time in 80% ethanolic extract ofDracocephalum heterophyllumB. through HPLC coupled to UV detector after optimization of extracting solvent and chromatographic conditions. Total flavonoids quantified were 0.324 mg/mL of the extract. HPLC analysis delivered contents of the luteolin, kaempferol, diosmetin, and chrysosplenetin as 0.08%, 0.14%, 0.28%, and 0.79% of the dried extract, respectively. LOD (%) values calculated were 0.04, 0.03, 0.03, and 0.08 and LOQ (%) values were 0.08, 0.12, 0.11, and 0.28 for luteolin, kaempferol, diosmetin, and chrysosplenetin, respectively. The recovery percentages for these flavonoids were within the acceptable range of 95% to 105%. Standard deviation and %RSD were calculated for each target analytes individually in extract for determining the reproducibility and accuracy of the method. In no case the %RSD was higher than...
Journal of the Brazilian Chemical Society, 2013
Um método racional e seletivo de cromatografia de alta eficiência acoplado à espectrometria de massas (CLAE-EM) com ionização por electrospray e analisador do tipo quadrupolo em tempo-de-voo em sequência (ESI-QToF-MS/MS) foi desenvolvido para a desreplicação de derivados fenólicos presentes em Qualea grandiflora e Qualea cordata. Os extratos para a análise foram selecionados por meio dos resultados de atividade antioxidante revelados no ensaio in vitro com DPPH. A análise por HPLC-ESI-QToF-MS/MS foi realizada por detecção contínua das relações massa/carga em alta resolução e pela técnica MS/MS de colisão induzida para fragmentação dos íons moleculares selecionados. A desreplicação da fração AcOEt do extrato hidroalcoólico dos caules de Q. grandiflora permitiu a detecção dos flavonoides: 3',4',5',5,6,7-hexahidroxi-8-metilflavanona, 8-metil-naringenina e 3',7-dimetoxi-8-metil-4',5,7-trihidroxiflavanona, assim como os derivativos da benzofenona (bis(4,6-dimetoxi-2-hidroxi-3-metilfenil)-metanona, 3',4'-dimetoxi-8-metil-5,6,7-trihidroxiflavanona, 7-metoxi-6-metil-3',4',5-trihidroxiflavanona, 6,8-dimetil-3'-metoxi-4',5,7-trihidroxiflavanona e 3',5'-dimetoxi-6,8-dimetil-4',5,7-trihidroxiflavanona) foram detectados na fração AcOEt do extrato hidroalcoólico das folhas de Q. cordata. A rational and selective method using on-line high-performance liquid chromatography (HPLC) coupled with electrospray quadrupole time-of-flight tandem mass spectrometry (ESI-QToF-MS/MS) was established for the dereplication of phenolic derivatives from Qualea grandiflora and Qualea cordata. The selection of the extracts was based on the antioxidant capacity measured by in vitro DPPH assay. The HPLC-ESI-QToF-MS/MS analysis was conducted by on-flow detection, using high-resolution mass/ratio ions as well as collision induced MS/MS experiments for selected protonated ions. The dereplication of the EtOAc fraction from the hydro-alcohol extract from the stem bark of Q. grandiflora allowed the detection of the flavonoids: 3
Comparative Study of Phenolic Content of Some Plant Extracts
Banat's Journal of Biotechnology, 2012
The aim of the present study was to draw a comparative analysis of the content in flavones and polyphenols of the hydro alcoholic extracts obtained from two medicinal plants, namely artichoke (leaves) and respectively, licorice (root) according to the origin of the vegetal material and the method used in the preparation of the extracts. The plants used in the experiments are two indigenous medicinal plants belonging to wild and cultivated flora in Romania. Artichocke (Cynara scolymus) and licorice (Glycyrrhiza glabra) were purchased from three local companies trading medicinal plants: Plafar, Vitaplant and Franco Impex. It was evaluated the composition of the extracts in terms of the content of polyphenolic compounds expressed in caffeic acid and flavone compounds content expressed in rutin. Plant extracts were obtained by two different methods of preparation: ultrasonation and maceration with intermittent shaking.
Revista Brasileira de Botânica, 2007
-(Detection of anthraquinones and identification of 1,4-naphthohydroquinone in cell suspension cultures of Rudgea jasminoides (Cham.) Müll. Arg. (Rubiaceae)). In Rubiaceae, anthraquinones and naphthoquinones are secondary metabolites characteristic of the subfamily Rubioideae, in which Rudgea jasminoides is included. Thin-layer chromatography using specific solvent systems and spray reagents indicated the presence of anthraquinones constitutively produced by cell suspension cultures of R. jasminoides. GC/MS analysis detected 1,4-naphthohydroquinone as a product of biosynthesis only after elicitation of the cells with yeast extract (Saccharomyces cerevisiae). The latter compound is probably a phytoalexin produced by suspension cultures of R. jasminoides. Key words-cell suspension culture, elicitor, naphtoquinones, Rubiaceae RESUMO-(Detecção de antraquinonas e identificação de 1,4-naftohidroquinona em suspensões celulares de Rudgea jasminoides (Cham.) Müll. Arg. (Rubiaceae)). Em Rubiaceae, antraquinonas e naftoquinonas são metabólitos secundários que caracterizam quimicamente a subfamília Rubioideae, na qual Rudgea jasminoides é incluída. Análises por cromatografia em camada delgada usando sistemas de solventes e reveladores específicos indicaram a presença de antraquinonas sintetizadas constitutivamente por suspensões celulares de R. jasminoides. Análises de GC/MS detectaram a presença de 1,4-naftohidroquinona como produto de biossíntese apenas após a eliciação das células com extrato de leveduras (Saccharomyces cerevisiae). Esta substância é provavelmente uma fitoalexina produzida por suspensões celulares de R. jasminoides.
JPC - Journal of Planar Chromatography - Modern TLC, 2011
A simple and sensitive high-performance thin-layer chromatographic (HPTLC)-densitometric method was developed for the simultaneous quantification of two flavonoid compounds, persicogenin and homoeriodictyol, in the methanol extracts of the aerial parts of two species (Rhus retinorrhoea and Rhus tripartita) of the genus Rhus grown in the Kingdom of Saudi Arabia. Chromatography was performed on glass-backed silica gel 60 F 254 HPTLC plates using toluene-ethyl acetate-methanol (8:2:0.5, v/v) as the mobile phase. Scanning and quantification were done at 293 nm. The system was found to give compact spot for homoeriodictyol and persicogenin at, R F = 0.30 ± 0.01 and 0.48 ± 0.01, respectively. The linearity ranges for homoeriodictyol and persicogenin were found to be the same (100-800 ng spot −1 ) with correlation coefficients (r 2 values) of 0.9989 and 0.9983, respectively. The limit of detection (LOD) for homoeriodictyol and persicogenin was found to be 26 and 31 ng band −1 , respectively, while the limit of quantification (LOQ) was found to be 77 and 92 ng band −1 , respectively. Homoeriodictyol (7.06%) and persicogenin (2.33%) were only found in R. retinorrhoea. The developed method was found to be accurate and precise; hence, it can be used as an important tool to assure the therapeutic dose of homoeriodictyol and persicogenin in herbal formulations as well as for the standardization and quality control of bulk drugs.