Characterization of Helicobacter species isolated from humans and dogs in Sharkia Governorate, Egypt. (original) (raw)

Characterization of Helicobacter species isolated from humans and dogs in Sharkia Governorate, Egypt Mohamed, M. E. M.; Suelam, I. I. A. and Saleh, M. A. (1) Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University, Egypt. (2) Laboratory Animal Unit, Veterinary Hospital, Zagazig University, Egypt (3) Animal Health research Institute, Dokki, Giza, Egypt Abstract The present work was carried out to study the occurrence of Helicobacer species in humans and dogs in Sharkia Governorate and characterization by antibiogram and Polymerase Chain Reaction. A total number of 35 human gastric biopsy samples were collected endoscopy Unit, Department of Internal Medicine, Faculty of Medicine, Zagazig University and 30 samples were collected from stray dogs (20 male and 10 female). Both Helicobacter pylori and Helicobacter heilmannii occur in the stomach of examined human subjects with the frequencies of 40% (14/35) and 8.6% (3/35), respectively. Helicobacter pylori occurs in peptic ulcers and apparently healthy cases with the frequencies of 44% (11/25) and 30% (3/10), respectively, moreover, Helicobacter heilmannii occurs with the frequencies of 12% (3/25) and zero% (0/10), respectively. Helicobacter pylori and H. heilmannii occurs in the stomach of examined dogs with the frequencies of 6.7% (2/30) and 33.3% (10/30), respectively. H. pylori occurs in gastroenteritis and apparently healthy cases with the frequencies of 7.1% (1/14) and 6.3 %( 1/16), respectively. Nevertheless, H. heilmannii occurs with the frequencies of 42.9 (6/14) and 25 (4/16), respectively. Representative strains of Helicobacter species were confirmed by PCR technique. The used primers were Helicobacter genus-specific PCR for 16S rRNA gene. Concerning the antibiotic sensitivity test, it was found that multi-drug resistance phenomena are emerged in Helicobacter species. The public health significance of the isolated Helicobacter species was discussed. The obtained results reveal that dogs in Sharkia Governorate are potential reservoir for Helicobacterioses.