Intertypic genomic rearrangements of poliovirus strains in vaccinees (original) (raw)

Abstract

In vivo intertypic RNA recombination has previously been observed in excreted type 3 poliovirus isolates from a normal asymptomatic primary vaccinee. This study examines isolates from additional primary vaccinees to determine whether intertypic recombination is a general occurrence in excreted polioviruses. T1 RNase oligonucleotide finger-printing and limited dideoxy primer extension RNA sequencing demonstrated no evidence of intertypic recombination among type 1 or type 2 excreted strains. However, vaccinees excreting type 3 strains for long periods of time eventually produced recombinant strains involving either type 1 or type 2 poliovirus. Moreover, a characteristic time course of appearance of excreted type 3 intertypic recombinant polioviruses was established. Type 3/type 1 and type 3/type 2 recombinant strains appeared at Days 10-11 with a single crossover site in the gene for nonstructural protein 2C. Type 3/type 2/type 3 complex recombinant strains with an additional crossov...

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References (23)

  1. COOPER, P. D. (1977). Genetics of picornaviruses. In "Comprehen- sive Virology" (H. Fraenkel-Conrat and R. R. Wagner, Eds.), Vol. 9, pp. 133-207. Plenum, New York.
  2. DORNER, A. J., SEMLER, E}. L., JACKSON, R. J., HANECAK, R., DUPHREY, E., and WIMMER, E. (1984). In vitro translation of poliovirus RNA: Utilisation of internal initiation sites in reticulocyte lysate. J. Virol. 50, 507-514.
  3. EVANS, D. M. A., DUNN, G., MINOR, P. D., SCHILD, G. C., CANN, A. J., STANWAY, G., ALMOND, J. W., CURREY, K., and MAIZEL, J. V. (1985). A single nucleotide change in the 5' non-coding region of the ge- nome of the Sabin type 3 poliovaccine is associated with increased neurovirulence. Nature (London) 314, 548-550.
  4. HIRST, G. (1962). Genetic recombination with Newcastle disease vi- rus, poliovirus and influenza. Cold Spring Harbor Symp. Quant. BioL 27,303-309.
  5. HOGLE, J. M., CHOW, M., and FILMAN, D. J. (1985). Three dimensional structure of poliovirus at 2.9, &, resolution. Science 229, 1358- 1365.
  6. KEW, O. M., and NOqq-AY, B. K. (1984). Evolution of the oral polio vac- cine strains in humans occurs by both mutation and intramolecular recombination. In "Modern Approaches to Vaccines: Molecular and Chemical Basis of Virus Virulence and Immunogenicity" (R. M. Chanock and R. A. Lerner, Eds.), pp. 357-362. Cold Spring Harbor Laboratory, Cold Spring Harbor, MY.
  7. KING, A. M. Q., MOCAHON, D., SAUNDERS, K., NEWMAN, J. W. [., and SLADE, W. R. (1985). Multiple sites of recombination within the RNA genome of foot-and-mouth disease virus. Virus Res. 3, 373 384.
  8. KING, A. M. Q., MCCAHON, D., SLADE, W. R., and NEWMAN, J. W. I. (1982). Recombination in RNA. Cell 29, 921-928.
  9. KING, A. M. Q., ORTLEPP, S. A., NEWMAN, J. W. [., and MCCAHON, D. (1987). Genetic recombination in RNA viruses. In "The Molecular Biology of the Positive Strand RNA Viruses" (D. J. Rowlands, M. A. Mayo, and B. W. J. Mahy, Eds.), FEMS Symposium No. 32, pp. 129-152. Academic Press, New York.
  10. KITAMURA, N., SEMLER, B. h., ROTHBERG, P. e., LARSEN, G. R., ADLER, C. J., DORMER, A. J., EMINI, E. A., HANECAK, R., LEE, J. L, VANDER WERF, S., ANDERSON, C. W., and WIMMER, E. (1981). Primary struc- ture, gene organisation and polypeptide expression of polievirus RNA. Nature (London) 291,547-553.
  11. LEDINKO, N. (1963). Genetic recombination with poliovirus type 1: Studies of crosses between a normal horse serum-resistant mu- tant and several guanidine-resistant mutants of the same strain. Virology 20, 107-119.
  12. MCCAHON, D. (1981 ). The genetics of aphthoviruses. Arch. Virol. 69, 1- 23.
  13. MOCAHON, D., KING, A. M. Q., ROE, D. S., BLADE, W. m., NEWMAN, J. W. I., and CLEARY, A. M. (1985}. Isolation and biochemical char- acterisation of intertypic recombinants of foot-and-mouth disease virus. Virus Res. 3, 87-100.
  14. MINOR, P. I~. (1980). Comparative biochemical studies of type 3 po- liovirus. J. ViroL 34, 73-84.
  15. MINOR, P. D. (1982). Characterisation of strains of type 3 polievirus by oliogonucleotide mapping..L Gen. Virot. 59, 307-317.
  16. MINOR, P, D,, and DUNN, G. (1988), The effect of sequences in the 5' non-coding region on the replication of polioviruses in the human gut../.
  17. Gen, Virot. 69, 1091-1096.
  18. MINOR, P. D., JOHN, A., FERGUSON, M., and ICENOGLE, J. P. (1986). Antigenic and molecular evolution of the vaccine strain of type 3 poliovirus during the period of excretion by a primary vaccinee. J. Gen. ViroL 67, 693-706.
  19. NOMOTO, A., KITAMURA, N., LEE, J. J., ROTHBERG, P. G., IMURA, N., and WIMMER, E. (1981 ). Identification of point mutations in the genome of the poliovirus Sabin vaccine LSc 2ab, and catalogue of RNase T1-and RNase A-resistant oligonucleotides of poliovirus type 1 (Mahoney) RNA. Virology 112, 2t7-227.
  20. ROMANOVA, L. t., TOLSKAYA, E. A., KOLESNIKOVA, M. S,, and AGOL, V. I. (1980). Biochemical evidence for intertypic recombination of poliovirus. FEBS Lett. 118, 109-112.
  21. ROSSMANN, M. G., ARNOLD, E., ERICKSON, J. W., FRANKENBERGER, E. A., GRIFFITH, J. P., HECHT, H.-J., JOHNSON, J. E., KAMER, G., LUO, M., MOSSER, A. G., RUEOKERT, R. R., SHERRY, B,, and VRIEND, G. (1985). Structure of a human common cold virus and functional relationship to other picornaviruses. Nature (London) 317, 145- 153. STANWAY, G., HUGHES, P. J., MOUNTFORD, R. C., REEVE, P., MINOR, P. D., SCHILD, G. C., and ALMOND, J. W. (1984). Comparison of the complete nucleotide sequences of the genomes of the neuroviru- lent poliovirus P3/Leon/37 and its attenuated Sabin vaccine deriva- tive P3/Leon/12alb. Proc. Natl. Acad. ScL USA 81, 1539-1543.
  22. TOLSKAYA, E. A., ROMANOVA, L. I., BLINOV, V. M., VIKTOROVA, E. G., SINYAKOV, A. N., KOLESNIKOVA, M S., and AGOL, V. I. (1987). Stud- ies on the recombination between RNA genomes of poliovirus: The primary structure and non-random distribution of crossover regions in the genomes of intertypic poliovirus recombinants. Virol- ogy161, 54-61.
  23. TOYODA, 14, KOHARA, M., KATAOKA, Y., SUGANUMA, T., OMATA, T., IM- URA, N., and NOMOTO, A. (1984). Complete nucleotide sequence of all three poliovirus serotype genomes: Implications for genetic relationship, gene function and antigenic determinants. J. MoL BioL 124, 561-585.