Pharmacokinetics of Casiopeína IIgly in Beagle Dog: A Copper Based Compound with Antineoplastic Activity (original) (raw)

Journal of Bioanalysis & Biomedicine, 2010

Abstract

ABSTRACT Casiopeína IIgly is a mixed chelate coordination complex with copper core that have demonstrated high antineoplastic activity in vitro and in vivo .In the present work, a developed and validated method for measurement of Casiopeína IIgly in beagle dog and its application in a pharmacokinetic study is reported. The analyte was isolated from blood by solid-phase extraction using Strata X cartridges. The analysis was carried out on a Synergy Polar-RP column (30 X 2.0 mm) using an isocratic elution and MeOH/ HFBA 0.1% (4:6) as the mobile phase. An Agilent LC/ MSD Trap VL equipped with an ionization electrospray source, was operated in selective ion storage (SIS) using stable ion [Cu(II) (F7C3COO) 4,7-dimethyl phen ]+ result of ESI reaction between Casiopeína IIgly and HFBA. The method demonstrated to be linear (r = 0.9992) in the range from the 0.1 to 15 μg/ml with a limit of detection (LOD) of 50 ng/ml. All the parameters of validation such as selectivity, accuracy, precision and recovery were within the required limits. Pharmacokinetics assay was carried out at 2 doses, indicated a high elimination rate.

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