Gating and regulation of connexin 43 (Cx43) hemichannels (original) (raw)
2003, Proceedings of the National Academy of Sciences
Connexin 43 (Cx43) nonjunctional or ''unapposed'' hemichannels can open under physiological or pathological conditions. We characterize hemichannels comprised of Cx43 or Cx43-EGFP (Cx43 with enhanced GFP fused to the C terminus) expressed in HeLa cells. Channel opening was induced at potentials greater than ؉60 mV. Open probability appeared to be very low. No comparable opening was detected in the parental, nontransfected HeLa cells. Conductance of fully open single hemichannels was Ϸ220 pS, which is approximately double that of Cx43 cell-cell channels. Cx43 hemichannels exhibited two types of gating: fast transitions (<1
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