Antioxidant Activities and Cytotoxicity of Zingiber zerumbet (L.) Smith Rhizome (original) (raw)
Related papers
Antioxidant Activity Study of Zingiber Zerumbet Rhizome Extract and Fraction
ad-Dawaa' Journal of Pharmaceutical Sciences, 2022
The Zingiber zerumbet was identified as a member of the Zingiberaceae family with pharmacological potential. This study aims to characterise the phytochemical compound and evaluate the antioxidant potential of the methanol extract, ethyl acetate, and n-hexane-fractions of Zingiber zerumbet rhizome. Maceration with methanol was used for extraction, and the extract was then fractionated with n-hexane, ethyl acetate, and water. The phytochemical identification was conducted for alkaloids, saponins, flavonoids, polyphenols, and steroids/triterpenoids. Antioxidant activity was determined based on DPPH absorbance measurements using UV-Vis spectrophotometry. The methanolic extract contained alkaloids, flavonoids, polyphenols, and steroids/triterpenoids. The ethyl acetate fraction contained flavonoids and polyphenols, and the n-hexane fraction contained alkaloids and flavonoids. The free radical scavenging activities of ascorbic acid, methanol extract, ethyl acetate, and the n-hexane soluble fraction were 7.79; 100,956; 69,476, and 77,780 μg/mL, respectively. The findings indicated that the methanolic extract, ethyl acetate, and n-hexane soluble fraction contained antioxidant compounds.
Antioxidant activity of a ginger extract (Zingiber officinale)
Food Chemistry, 2007
The antioxidant effect and the total phenols of ginger extract were studied. The total phenols of the alcohol extract were found to be 870.1 mg/g dry extract. 2,2-Diphenyl-1-picril hydrazyl radical (DPPH) scavenging reached 90.1% and exceeded that of butylated hydroxytoluene (BHT), the IC 50 concentration for inhibition of DPPH was 0.64 lg/ml. The antioxidant activity in a linoleic acid/water emulsion system determined by means of thiobarbituric acid reactive substances (TBARS) was highest at 37°C-73.2%, and 71.6% when the formation of conjugated dienes was inhibited. At 80°C the antioxidant activity at the highest concentration of a ginger extract was less efficient: 65.7% for conjugated dienes formation and 68.2% for TBARS. The ginger extract inhibited the hydroxyl radicals 79.6% at 37°C and 74.8% at 80°C, which showed a higher antioxidant activity than quercetin. The IC 50 concentration for inhibiting OH Å at 37°C was slower than that at 80°C-1.90 and 2.78 lg/ml, respectively. The ginger extract chelated Fe 3+ in the solution.
IN VITRO EVALUATION OF ANTIOXIDANT ACTIVITY OF GINGER (Zingiber Officinale)
Find out the scientifically evidence of Ginger (Zingiber Officinale) for it’s antioxidant property. Preliminary phytochemical screening and in-vitro antioxidant activity of Ginger (Zingiber Officinale) extract were investigated but the extraction was done at different temperature( 35ºC,60ºC,100ºC) by decoction process. The antioxidant activity was studied in some in-vitro antioxidant models like DPPH radical scavenging activity, superoxide radical scavenging activity, ferric reducing power and hydrogen peroxide scavenging activity. Total antioxidant capacity was also determined. The Ginger (Zingiber Officinale) extract showed antioxidant activity by inhibiting DPPH, scavenging superoxide and hydrogen peroxide. It also showed reducing power ability in ferric reducing model. Total antioxidant capacity was found to be 18.32 mg/gm expressed as L-Ascorbic acid. Significant antioxidant activity of Water extract of Ginger (Zingiber Officinale) was found which might be due to the presence of Acidic compounds, Flavonoids, Phenols, Saponins, Tannins (Phenolic compounds) and Triterpenoids found in the preliminary Phytochemical screening. Key words: Antioxidant, Ginger, Ferric Reducing, Zingiberaceae
Sains Malaysiana
Zingiber zerumbet is widely used as therapeutic agent in traditional medicine and is reported to exert antioxidant activities. This study evaluated in vitro antioxidant potential of Z. zerumbet rhizome ethyl acetate extract on hydrogen peroxide (H 2 O 2)-induced red blood cells (RBCs) damage; by measuring percentage of haemolysis, oxidative damage and morphological changes in treated RBCs. On a preliminary dose-response analysis, it was observed that incubation of RBCs with extract at doses of 50, 25, 12.5 and 6.25 µg/mL all had no significant effect on RBCs similar to NaCl control and BHT (positive control), therefore, these concentrations were used in the subsequent study. The RBCs were pre-incubated with extract in the chosen concentrations, prior to treatment with H 2 O 2. The results showed that only 6.25 µg/mL+H 2 O 2 group showed significantly (p<0.05) lower percentage of haemolysis and oxidative damage; indicated by low level of malondialdehyde (MDA) and protein carbonyls (PC) compared to the H 2 O 2 alone group. Electron microscopic examination further showed that pre-treatment with 6.25 µg/mL extract reduced the H 2 O 2-induced morphological changes of RBCs. Phytochemical analysis of extract using Gas Chromatography Mass Spectrometry (GCMS) identified Zerumbone as the highest constituent compound (51.57%) in this extract. In conclusion, this study indicated that 6.25 µg/mL ethyl acetate extract Z. zerumbet rhizome could efficiently protect RBCs against oxidative damage induced by H 2 O 2 and this effects could possibly due the high constituent of Zerumbone.
Scientific Reports
Due to having a long history of traditional uses as a functional food, Zingiber zerumbet was selected here to explore the inherent antioxidant and antineoplastic activities of methanolic extract of its rhizome (MEZZR) against Ehrlich ascites carcinoma (EAC) cells. The rich polyphenol containing MEZZR showed a marked DPPH, ABTS, nitric oxide radicals and lipid peroxidation inhibition activity with an IC50 of 3.43 ± 1.25, 11.38 ± 1.39, 23.12 ± 3.39 and 16.47 ± 1.47 µg/ml, respectively, when compared to the standard catechin. In vivo, MEZZR significantly inhibited EAC cell growth, decreased body weight gain, increased life span and restored the altered hematological characteristics of EAC-bearing mice. Moreover, MEZZR induced nuclear condensation and fragmentation, which are notable features of apoptosis as observed by fluorescence microscopy after staining EAC cells of MEZZR-treated mice with Hoechst 33342. Additionally, in vitro, the cell growth inhibition caused by the MEZZR in MTT ...
Evaluation of cytotoxicity and antioxidant properties of some Zingiberaceae plants
International Journal of Green Pharmacy, 2019
Aim: Zingiberaceae family is widely distributed in the tropical realm of Asia. Considering its diverse applications as spices and therapeutics, the present study was undertaken to evaluate the cytotoxic and antioxidant effect of the ethanolic rhizome extracts of five plants, namely Alpinia galanga (L.) Willd., Alpinia zerumbet (Pers.) B.L. Burtt and R. M. Smith, Curcuma caesia Roxb., Zingiber officinale Rosc., and Zingiber zerumbet (L.) Smith on Allium cepa Linn. system. Materials and Methods: Cytotoxicity was evaluated by 2,3,5-triphenyltetrazolium chloride (TTC) and 2’,7’-dichlorofluorescein diacetate (DCFDAH2) assays. Further, in vitro DNA protection assay was performed to confirm the antioxidant potentials of the extracts. Characterization of phytochemicals was done by performing qualitative tests. Results and Discussion: TTC reduction assay revealed that the extracts (2.5, 5, and 10 μg/ml) had no cytotoxic effect on A. cepa root cells. Roots treated with extracts (2.5 μg/ml) were stained with reactive oxygen species-sensitive dye DCFDAH2 and visualized under the fluorescence microscope. The result confirmed that the extracts did not exert any prooxidant effect. Further, the extracts established their substantial antioxidant potential by inhibiting oxidative DNA damage in an in vitro system. In addition, qualitative analysis showed that the rhizomes are rich in phytochemicals. Conclusion: From the current observations, it can be concluded that the selected herbs can be utilized safely for human consumption.
Antitumoral activity, antioxidant capacity and bioactive compounds of ginger (Zingiber officinale)
Acta Scientiarum. Technology, 2019
Zingiber officinale, popularly known as ginger, it is in National Relations of Medicinal Plants of Interest to SUS (RENISUS) aiming at the appropriate and safe use of medicinal plants. Then, it is necessary to characterize and determine their bioactive compounds, their cytotoxic, mutagenic and/or antitumor properties, in order to guarantee food safety and its beneficial coadjuvant effects. The ginger rhizomes were dried at temperatures 40, 60, and 80°C and the bioactive compounds were extracted with methanol and ethanol (70:30 and 95:5). The rhizomes showed to be intermediate source of vitamin C. The highest levels of anthocyanins and yellow flavonoids were dried at 40°C. The highest content of phenolic compounds was for the extracts MeOH 70:30 and EtOH 70:30 at 80°C and for flavonoids MeOH 95:5 at 40°C. Data showed a positive correlation with the content of bioactive compounds and the antioxidant activity. The EtOH 70:30 extract at 80°C, did not present a cytotoxic effect, by calc...
Biological and chemical properties of Zingiber zerumbet Smith: a review
Numerous researches have been carried out in Zingiber zerumbet Smith. Since 1944 till date. Z. zerumbet is a monocotyledonous perennial medicinal plant belonging to Zingiberaceae family. It is commonly known as shampoo ginger. It has many different local names depending on their area of collection and vegetation. It is called as 'Singkha' in Manipuri. Various compounds have been reported to be isolated from Z. zerumbet and they serve a very potent and reliable drug candidate for the various diseases. They have been investigated for its prospects of effectiveness against number of activities in in vitro as well as in vivo and mechanisms that may be involved in chemo preventive measures and various pharmaceutical studies.
Chemical and Biological Technologies in Agriculture
Background Ginger is widely used in traditional food and folk medicine. It was known to contain monoterpenoids, sesquiterpenoids, phenolic compounds and its derivatives as well as aldehydes, ketones, alcohols, and esters. The current work aims to evaluate the antioxidant and anticancer activities of different extracts from ginger peels and residues. Methods Ginger samples were collected from local market and extracted using water and ethanol solvent. Four different antioxidant assays were used (DPPH, ABTS, potassium permanganate and methylene blue), the anticancer activity was determined using HepG2 cell line and the active ingredients for the promising extract were identified using HPLC/UV. Results The obtained results recorded that hot water extract of ginger peels was the promising extract exhibiting promising antioxidant activity. Fractionation of this promising extract was achieved by silica gel column chromatography with petroleum ether/ethyl acetate as mobile phase. Six fract...