Influence of Growth Parameters on the Formation of Hydroxyapatite (HAp) Nanostructures and Their Cell Viability Studies (original) (raw)
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A comparative study of functionalized nano-hydroxyapatite
2015 E-Health and Bioengineering Conference (EHB), 2015
In an attempt to obtain appropriate components for complex transfection systems hydroxyapatite nanoparticles (nHAp) were synthesized by means of wet chemical precipitation method, under atmospheric pressure, in the absence or presence of different low-molecular or polymeric cationic dispersants, i.e. arginine, linear or branched polyethylenimine (LPEI, bPEI). The effect of added compounds' type and concentration on nHAp characteristics (surface chemistry, size, morphology, crystallinity, dispersibility) was comparatively studied. The chemical composition and surface modification of the synthesized nano-HAp was investigated using Fourier Transformed Infrared Spectroscopy (FTIR), Energy Dispersive X-Ray (EDX) analysis, Zeta potential measurements (DLS-dynamic light scattering) and UV-vis spectrophotometry. The crystal morphology and particle size were characterized by means of transmission electron microscopy (TEM), scanning electron microscopy (SEM) and X-Ray Diffraction technique (XRD). The DNA binding ability of the functionalized nHAp was tested by agarose gel electrophoresis assay.
In the present work, we report environmental friendly green synthesis of carbonated hydroxyapatite (HAp) sheet like nanoparticles employing waste eggshells of the hen and zingiberacae (known as ginger) extract as the starting precursors. The structural and morphological analyses were carried out by using XRD, FTIR, Raman, FESEM and TEM. The characterization studies confirm the phase purity, size, crystalline nature and sheet like morphology of carbonated HAp. The thermal analysis using TG/DTA confirms the thermal stability of carbonated HAp nanosheets. Evaluation of in-vitro preosteoblasts cell viability evinces that the carbonated HAp nanosheets are biocompatible. The obtained results confirm that the carbonated hydroxyapatite (HAp) sheet like nanostructures are biocompatible and could be used as biomaterials for orthopedic and other biological applications. Further, the present findings open a new process for waste management (recycling), making green environment and preparing nano structured biomaterials from naturally available resources and medicinal plants for biological applications.
An Overview of Synthesis Based Biomedical Applications of Hydroxyapatite Nanomaterials
Journal of Nepal Chemical Society, 2021
Hydroxyapatite (HAp) is the mineral phase of animal bones embedded in the collagen-containing organic matrix of the bones. It is a naturally optimized material that provides physical support to the bones. Hydroxyapatitebased biomaterials, hence, find wide biomedical applications especially in orthopedics, dentistry, and tissue engineering due to their biocompatibility, bioactivity, osteoconductivity, and similar chemical composition of HAp to that of minerals present in animal bones. Different physicochemical synthetic methods and available natural biogenic sources have been reported for the preparation of nano-HAp. However, particle size, aspect ratio, morphology, crystallinity, and the distribution of HAp in biomaterials have significant effects on their biomedical applications. This paper has summarized some methods of extraction of nano-HAp from different biogenic sources including bio-wastes. Furthermore, it focuses on some facile wet chemical synthetic routes of preparing nano...
Nanoparticles of hydroxyapatite: preparation, characterization and cellular approach-An Overview
Nanostructure syntheses have been applied to derive hydroxyapatite in nanometric scale for orthopaedic and dental implant applications due to its biocompatibility and similarity to natural apatite characteristics, becoming to a preferred material compared to hydroxyapatite in micro scale. This overview presents some of the most studied processes of obtaining nanophased hydroxyapatite, enriched with results from our group and additional and interesting information about cell-nanohydroxyapatite performance in vitro experiments.
2009
The nano crystalline hydroxyapatite with highly uniform spherical morphology has successfully been prepared using the new cationic surfactant Cetrimide as template by co-precipitation method at ambient temperature. The sample was calcinated at 750 oC for 8h. The FTIR spectrum shows the chemical composition of HAP. The XRD pattern confirms the characteristic peaks and the hexagonal structure of HAP. The lattice parameters calculated from the XRD pattern are a = b ~ 0.93873 nm, c = 0.68486 nm, the axial ratio ~0.7296, the volume of the unit cell ~522.6535x10-30 m3. The size and morphology of the sample were analyzed by FESEM, which shows that the spherical HAp particles with diameter 150-200 nm have successfully been synthesized. All the inspections confirm the successful preparation of nano spherical HAP with excellent morphology control, and uniform size.
Characterization and influence of hydroxyapatite nanopowders on living cells
Beilstein Journal of Nanotechnology, 2018
Nanomaterials, such as hydroxyapatite nanoparticles show a great promise for medical applications due to their unique properties at the nanoscale. However, there are concerns about the safety of using these materials in biological environments. Despite a great number of published studies of nanoobjects and their aggregates or agglomerates, the impact of their physicochemical properties (such as particle size, surface area, purity, details of structure and degree of agglomeration) on living cells is not yet fully understood. Significant differences in these properties, resulting from different manufacturing methods, are yet another problem to be taken into consideration. The aim of this work was to investigate the correlation between the properties of nanoscale hydroxyapatite from different synthesis methods and biological activity represented by the viability of four cell lines: A549, CHO, BEAS-2B and J774.1 to assess the influence of the nanoparticles on immune, reproductive and re...
Irbm, 2010
Objective The aim of this work was to synthesize and to characterize chemically and biologically (in vitro and in vivo) a nano-sized hydroxyapatite (nHA). Materials and methods Wet chemical precipitation at room temperature was performed, then chemical structure was explored using transmission electron microscopy, X-ray diffraction and Fourier transformed infrared spectroscopy. In vitro biological characterization was done using MG63 osteoblastic cell line cultured onto the material, and characterization was done for morphology (scanning electron microscopy), viability (live/dead assay) and proliferation (MTT test). Finally, nHA powder was tested in vivo in a study involving C57 Black mice for bone repair in a calvarial bone critical sized defect. Results Morphological, physico-chemical and cristallographic analyses revealed specific features of hydroxyapatite. Biological in vitro experiments revealed high affinity and proliferative ability of MG63 cells cultured onto the material. ...
Growth and characterization of nano-crystalline hydroxyapatite at physiological conditions
Crystal Research and Technology, 2008
Pure, stable stoichimetric nano crystalline hydroxyapatite material was crystallized by double diffusion technique at physiological conditions, temperature at 37°C and pH at 7.4. The sample was sintered at 400°C, 750°C and 1200°C with equal interval of time. They were characterized by X-ray diffraction studies, Fourier Transformation Infra-Red analysis, Thermogravimetric analysis, Scanning Electron Microscopic studies and Atomic Force Microscopic studies. The X-ray analysis confirmed that the grown crystals are to be the pure form of hydroxyapatite. Infra-red studies confirmed CO free hydroxyapatite. Thermogravimetric studies showed the thermal stability of the hydroxyapatite crystals even at 1200°C. The presence of pores in the sintered sample was traced by scanning electron microscopy. Atomic force microscopy revealed the presence of nano crystalline HAP of size 0.958 nanometer in the samples grown using this technique. At higher temperature the deagglomeration of bulk phases and agglomeration of nano phases leads to the nano crystalline HAP were observed.
Preparation of Nanostructured Hydroxyapatite in Organic Solvents for Clinical Applications
2011
Composite materials consisting of Hydroxyapatite (HAP) nano-crystals and biocompatible polymers have been widely used in orthopedic and dental application. These composite can provide both ease of use and superior mechanical properties of polymers, osteoconductivity and bioactivity of HAP. The formation of HAP in different organic solvents and water was investigated to fabricate such composites at moderate temperatures. HAP nano-sized crystals with high degree of crystallinity were formed at room temperature by rapid addition of (NH 4) 2 HPO 4 solution into Ca(NO 3) 2 .4H 2 O solution, filtration and subsequent drying at 40 ºC. The effect of different organic solvents, addition mode and temperature on the characteristics of HAP was examined. The HAP powders were characterized using Fourier transform infrared spectrometer (FTIR), X-ray diffraction (XRD), and Transmission electron microscopy (TEM). The HAP crystal shape was governed by the type of solvent: fine regular spheres and rods were formed from organic solvents with lower dielectric constants while irregular particles were prepared using water with higher dielectric constant. The synthesized HAP rod crystals were between (20-100) nm length and (2-6) nm thickness. All the FTIR and XRD profiles of HAP fabricated from ethanol were in good agreement with the FTIR and XRD spectrums of commercial HAP. It is therefore feasible to fabricate HAP uniform nano-particles in organic solvents at room temperature, which would be of benefit for the fabrication of polymer-HAP composites for biomedical applications.