Gluconobacter nephelii sp. nov., an acetic acid bacterium in the {alpha}-Proteobacteria (original) (raw)
Three strains, RBY-1T, PHD-1, and PHD-2 were isolated from fruits in Thailand. The strains were Gram-negative, aerobic rods with polar flagella, produced acetic acid from ethanol and did not oxidize acetate and lactate. In phylogenetic trees based on 16S rRNA gene sequences and 16S-23S rRNA gene ITS sequences, the strains formed a cluster separate from the type strains of the eleven species of the genus Gluconobacter. The calculated 16S rRNA gene sequence and 16S-23S rRNA gene ITS sequence similarities were respectively 97.7-99.7% and 77.3-98.1%. DNA G+C contents were 57.2-57.6 mol%. They showed high DNA-DNA relatedness of 100% with one another but low DNA-DNA relatedness of 11-34% with the type strains of the eleven Gluconobacter species. Q-10 was a major quinone. On the basis of the genotypic and phenotypic data obtained, the three strains clearly represent a novel species, for which the name Gluconobacter nephelii sp. nov. is proposed. The type strain is strain RBY-1T (= BCC 3673...