Determination of metals in marine species by microwave digestion and inductively coupled plasma mass spectrometry analysis (original) (raw)

Abstract

A microwave digestion method suitable for determination of multiple elements in marine species was developed, with the use of cold vapor atomic spectrometry for the detection of Hg, and inductively coupled plasma mass spectrometry for all of the other elements. An optimized reagent mixture composed of 2 ml of HNO 3 , 2 ml of H 2 O 2 and 0.3 ml of HF used in microwave digestion of about 0.15 g (dry weight) of sample was found to give the best overall recoveries of metals in two standard reference materials. In the oyster tissue standard reference material (SRM 1566b), recoveries of Na, Al, K, V, Co, Zn, Se, Sr, Ag, Cd, Ni, and Pb were between 90% and 110%; Mg, Mn, Fe, Cu, As, and Ba recoveries were between 85% and 90%; Hg recovery was 81%; and Ca recovery was 64%. In a dogfish certified reference material (DORM-2), the recoveries of Al, Cr, Mn, Se, and Hg were between 90% and 110%; Ni, Cu, Zn, and As recoveries were about 85%; and Fe recovery was 112%. Method detection limits of the elements were established. Metal concentrations in flounder, scup, and blue crab samples collected from coastal locations around Long Island and in the Hudson River estuary were determined.

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  1. Developed a microwave digestion method for determining 27 metals in marine species using CVAAS and ICPMS.
  2. Optimized reagent mixture includes 2 ml HNO3, 2 ml H2O2, and 0.3 ml HF for best recoveries.
  3. Metal recoveries in oyster tissue SRM 1566b ranged from 64% to 112%, highlighting method effectiveness.
  4. Method detection limits established for all 27 elements, ensuring accurate measurements at health-criteria levels.
  5. Marine species from Long Island and Hudson River showed no exceedance of health-criteria levels for toxic metals.

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