Gelam Honey Protects against Gamma-Irradiation Damage to Antioxidant Enzymes in Human Diploid Fibroblasts (original) (raw)
2013, Molecules
https://doi.org/10.3390/MOLECULES18022200
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Abstract
The present study was designed to determine the radioprotective effects of Malaysian Gelam honey on gene expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of human diploid fibroblasts (HDFs) subjected to gamma-irradiation. Six groups of HDFs were studied: untreated control, irradiated HDFs, Gelam honey-treated HDFs and HDF treated with Gelam honey pre-, during-and post-irradiation. HDFs were treated with 6 mg
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Gelam honey acting as a radioprotectant agent in gamma-irradiated human diploid fibroblasts
Research Journal of Medicinal Plant
In this study, the effect of Gelam honey against radiation-induced DNA damage and cell survival rate of human diploid fibroblasts (HDFs) was evaluated. The degree of damaged DNA was determined by Comet assay while Clonogenic assay was used to evaluate cell survival rate. Irradiated Gelam honey at the concentration of 6 mg/ml was used to treat HDFs pre-, during- and post-exposure to 1 Gy of gamma-rays to evaluate its radioprotectant properties. Comet assay showed that exposure to gamma-rays caused a significant increase in total DNA damage in a dose dependent manner (p<0.05). Pre-treatment with Gelam honey at 6 mg/ml decreased the degree of damaged DNA significantly (p<0.05) which was not observed when Gelam honey treatment was given during- and post-irradiation. Clonogenic assay showed the percentage of survival fraction of HDFs decreased significantly with increasing dose of gamma-rays exposure (p<0.05). Cell survival rate however was significantly increased when HDFs were...
BMC Complementary and Alternative Medicine, 2014
Background: The interaction between ionizing radiation and substances in cells will induce the production of free radicals. These free radicals inflict damage to important biomolecules such as chromosomes, proteins and lipids which consequently trigger the expression of genes which are involved in protecting the cells or repair the oxidative damages. Honey has been known for its antioxidant properties and was used in medical and cosmetic products. Currently, research on honey is ongoing and diversifying. The aim of this study was to elucidate the role of Gelam honey as a radioprotector in human diploid fibroblast (HDFs) which were exposed to gamma-rays by determining the expression of genes and proteins involved in cell cycle regulation and cell death.
Protective Role of Royal Jelly Against Radiation-Induced Oxidative Stress in Rats
International Journal of Hematology and Oncology, 2013
The aim of this study was to investigate the effects of royal jelly against gamma-radiation induced oxidative damage in liver and lung tissue after total body irradiation. The adult male Sprague Dawley rats were randomly divided into six groups of sixteen animals each; group 1: control group (C), group 2: only irradiated rats (IR), group 3: irradiated rats with royal jelly administration at 25 mg/kg/day (IR+RJ25), group 4: irradiated rats with royal jelly administration at 50 mg/kg/day (IR+RJ50), group 5: only royal jelly administration at 25 mg/kg/day (RJ25), group 6: only royal jelly administration at 50 mg/kg/day (RJ50). Royal jelly (RJ) was administered at a dose of 25 and 50-mg/kg body weight, by gavage for 10 days prior to irradiation and 10 days after irradiation. On the tenth day of study, radiotherapy was applied to the whole-body by single fraction at a dose of 6 Gy. Half of rats were sacrificed at 24 hours and 10 days after irradiation under ether anesthesia. Blood samples were collected and analysed for alanine aminotransferase, aspartate aminotransferase, triygliceride, total cholesterol and gamma glutamyl transpeptidase levels. The lung and liver samples were stored for the measurement of malondialdehyde, glutathione peroxidase, superoxide dismutase and catalase activities. Rats exposed to wholebody irradiation induced a marked liver failure, characterized with a significant increase in serum AST, ALT, cholesterol and triglyceride concentrations, and also they had higher lung and liver MDA and lower GSH-Px, CAT and SOD (p<0.001). Administration of royal jelly resulted in a significant decreased in oxidative stress parameters and biochemical parameters, and certainly increased antioxidant activities. Furthermore, pre-and post-treatment with RJ was more effective than pre-treatment with RJ. These results suggest that royal jelly may probably protect the animals from radiation-induced liver and lung damage. The effects of royal jelly can result from its antioxidant properties.
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Two types of monofloral Malaysian honey (Gelam and Nenas) were analyzed to determine their antioxidant activities and total phenolic and flavonoid contents, with and without gamma irradiation. Our results showed that both types of honey can scavenge free radicals and exhibit high antioxidant-reducing power; however, Gelam honey exhibited higher antioxidant activity (p < 0.05) than Nenas honey, which is in good correlation (r = 0.9899) with its phenolic contents. Interestingly, we also noted that both irradiated honeys have higher antioxidant activities and total phenolic and flavonoid contents compared to nonirradiated honeys by Folin-Ciocalteu and UV-spectrophotometry methods, respectively. However, HPLC analysis for phenolic compounds showed insignificant increase between irradiated and nonirradiated honeys. The phenolic compounds such as: caffeic acid, chlorogenic acid, ellagic acid, p-coumaric acid, quercetin and hesperetin as indicated by HPLC method were found to be higher in Gelam honey versus Nenas honey. In conclusion, irradiation of honey causes enhanced antioxidant activities and flavonoid compounds.
This study was conducted to evaluate the effects of evaporation, gamma irradiation and temperature on the total polyphenols, flavonoids and 1,1-diphenyl-2picrylhydrazyl (DPPH) radical-scavenging activities of Tualang honey samples (n = 14) following storage over three, six or twelve months. The mean polyphenol concentrations of the six gamma irradiated honey samples at three, six and twelve months, respectively, were 96.13%, 98.01% and 102.03% higher than the corresponding values of the eight nongamma irradiated samples. Similarly, the mean values for flavonoids at three, six and twelve months were 111.52%, 114.81% and 110.04% higher, respectively, for the gamma irradiated samples. The mean values for DPPH radical-scavenging activities at three, six and twelve months were also 67.09%, 65.26% and 44.65% higher, respectively, for the
Oxidative medicine and cellular longevity, 2017
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Large doses of ionizing radiation can damage human tissues. Therefore, there is a need to investigate the radiation effects as well as identify effective and non-toxic radioprotectors. This study evaluated the radioprotective effects of Kelulut honey (KH) from stingless bee (Trigona sp.) on zebrafish (Danio rerio) embryos. Viable zebrafish embryos at 24 hpf were dechorionated and divided into four groups, namely untreated and non-irradiated, untreated and irradiated, KH pre-treatment and amifostine pre-treatment. The embryos were first treated with KH (8 mg/mL) or amifostine (4 mM) before irradiation at doses of 11 Gy to 20 Gy using gamma ray source, caesium-137 (137Cs). Lethality and abnormality analysis were performed on all of the embryos in the study. Immunohistochemistry assay was also performed using selected proteins, namely γ-H2AX and caspase-3, to investigate DNA damages and incidences of apoptosis. KH was found to reduce coagulation effects at up to 20 Gy in the lethality ...
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Ionizing radiation is known to induce oxidative stress through generation of reactive oxygen species (ROS) resulting in imbalance of the pro-oxidant and antioxidant activities ultimately resulting in cell death. Ferulic acid (FA) is a phytochemical commonly found in fruits and vegetables such as tomatoes, sweet corn, and ricebran. FA exhibit a wide range of pharmacological effects including antiageing, anti-inflammatory, anticancer, antidiabetic, antiapoptotic, and neuroprotective. The present work is aimed at evaluating the radioprotective effect of FA, on ␥-radiation induced toxicity in primary cultures of isolated rat hepatocytes. Hepatocytes were isolated from the liver of rats by collagenase perfusion. The cellular changes were estimated using lipid peroxidative indices like thiobarbituric acid reactive substances (TBARS), the antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH), ceruloplasmin, Vitamins A, E and C and uric acid. DNA damage was analyzed by single cell gel electrophoresis (comet assay). An increase in the severity of DNA damage was observed with increasing dose (1, 2 and 4 Gy) of ␥-radiation in cultured hepatocytes. TBARS were increased significantly, whereas the levels of GSH, Vitamins C, E and A, ceruloplasmin, uric acid and antioxidant enzymes were significantly decreased in ␥-irradiated groups. The maximum damage to hepatocytes was observed at 4 Gy irradiation. Pretreatment with FA (1, 5 and 10 g/ml) significantly decrease the levels of TBARS and DNA damage. In addition, pretreatment with FA significantly increased antioxidant enzymes, GSH, Vitamins A, E and C, uric acid and ceruloplasmin levels. The maximum protection of hepatocytes was observed at 10 g/ml of FA pretreatment. Thus, pretreatment with FA helps in protecting the hepatocytes against ␥-radiation induced cellular damage and can be developed as a effective radioprotector during radiotherapy.
Royal jelly modulates oxidative stress and tissue injury
Background: Royal jelly is a nutritive secretion produced by the worker bees, rich in proteins, carbohydrates, vitamins and minerals. Aim: The present study was designed to determine the possible protective effects of royal jelly against radiation induced oxidative stress, hematological, biochemical and histological alterations in male Wister albino rats. Materials and Methods: Male Wister albino rats were exposed to a fractionated dose of gamma radiation (2 Gy every 3 days up to 8 Gy total doses). Royal jelly was administrated (g/Kg/day) by gavages 14 days before exposure to the 1 st radiation fraction and the treatment was continued for 15 days after the 1 st irradiation fraction till the end of the experiment. The rats were sacrificed 3 rd , equivalent to 3rd post 2nd irradiation fraction, and equivalent to 3rd day post last irradiation fraction. Results: In the present study, gamma-irradiation induced hematological, biochemical and histological effects in male Wister albino rats. In royal jelly treated irradiated group, there was a noticeable decrease recorded in thiobarbituric reactive substances concentration when compared to γ-irradiated group. Also, the serum nitric oxide concentration was significantly improved. The administration of royal jelly to irradiated rats according to the current experimental design significantly ameliorates the changes induced in serum lipid profile. Moreover, in royal jelly treated irradiated group, there was a noticeable amelioration recorded in all hematological parameters along the three experimental intervals. The microscopic examination of cardiac muscle of royal jelly treated irradiated rats demonstrated structural amelioration, improved nuclei and normal features of capillaries and veins in endomysium when compared to gamma-irradiated rats. Conclusion: It was suggested that the biochemical, hematological and histological amelioration observed in royal jelly (g/Kg/day) treated irradiated rats might be due to the antioxidant capacity of royal jelly active constituents.
Toxicology, 2006
Ionizing radiation is known to induce oxidative stress through generation of reactive oxygen species (ROS) resulting in imbalance of the pro-oxidant and antioxidant activities ultimately resulting in cell death. Ferulic acid (FA) is a phytochemical commonly found in fruits and vegetables such as tomatoes, sweet corn, and ricebran. FA exhibit a wide range of pharmacological effects including antiageing, anti-inflammatory, anticancer, antidiabetic, antiapoptotic, and neuroprotective. The present work is aimed at evaluating the radioprotective effect of FA, on gamma-radiation induced toxicity in primary cultures of isolated rat hepatocytes. Hepatocytes were isolated from the liver of rats by collagenase perfusion. The cellular changes were estimated using lipid peroxidative indices like thiobarbituric acid reactive substances (TBARS), the antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH), ceruloplasmin, Vitamins A, E and...
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In the present study, we aimed to examine the antioxidant, antiaging and photoprotective properties of Greek honey samples of various botanical and geographical origin. Ethyl-acetate extracts were used and the and the total phenolic/flavonoid content and antioxidant capacity were evaluated. Honey extracts were then studied for their cytoprotective properties against UVB-induced photodamage using human immortalized keratinocytes (HaCaT) and/or reconstituted human skin tissue models. Specifically, the cytotoxicity, oxidative status, DNA damage and gene expression levels of specific matrix metalloproteinases (MMPs) were examined. Overall, the treatment of HaCaT cells with honey extracts resulted in lower levels of DNA strand breaks and attenuated the decrease in cell viability following UVB exposure. Additionally, honey extracts significantly decreased the total protein carbonyl content of the irradiated cells, however, they had no significant effect on their total antioxidant status. ...
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Journal of Food Measurement and Characterization, 2021
Honey, rich with polyphenols, vitamins, proteins, organic acids and minerals, and is supersaturated solution of sugars. Gamma irradiation could be a physical food preservation technique which protects against food related-insects and microbial contamination and increases the shelf life of some products. The present research was investigated the effect of γ-irradiation (0.0, 2.5, 5.0 and 10.0 kGy) on the physicochemical possessions of the substances of honey; phenolic and flavonoids content in addition to the antioxidant activity by DPPH radical. Diastase No. is also identified with and without gamma irradiation. After γ-irradiation a minor decrease in moisture content was observed while color intensity was significantly increased by elevating irradiation dosage height mainly dose intensity 10.0 kGy which gave the premier color intensity compared with control. But there was no significant difference in the pH values and total soluble solids (TSS%) as well as sugars% of honey samples. Significant increases within the phenolic content were observed with the use of γ-rays particularly at dose 10.0 kGy which gave the main content relative to the control, while a trivial increase in the flavonoid content was obtained within the rise of the γ-irradiation dose. The findings have also shown that free radicals can be scavenged by honey and reveal high activity of antioxidants. Hydroxymethylfurfural is reduced by raising the irradiation dose, in converse diastase No. is enlarged by amplifying the level of the γ-rays. Also, the results showed that the inhibition zones varied according to the irradiation dose level, the dose level 10.0 kGy was more effective for inhibiting the growth of both Gram-positive and Gram-negative bacteria as well as fungal development. The lung cancer cells (A549) showed a decrease in cell viability and density, irradiated honey with dose level 10.0 kGy gave the lowest IC 50 (6.08 mg/ml). Honey treated with a dose of 10.0 kg of gamma rays did not affect the physical and biochemical properties of honey and was more effective as an antimicrobial and anti-lung cancer, and the quality of honey remained unchanged upon radiation treatment.
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Madu gelam telah didapati mempunyai kesan anti-oksidatif, anti-kanser dan antiinflamasi terhadap banyak jenis kanser. Tujuan kajian ini adalah untuk menentukan kesan madu gelam terhadap aras tekanan oksidatif sel kanser peparu manusia. IC 50 madu gelam ditentukan dengan merawat sel A549 dengan dos madu yang berbeza (50-200 mg/ml). Sel dibahagikan kepada 4 kumpulan dan diaruh tekanan oksidatif dengan menggunakan hidrogen peroksida (H 2 O 2 ) mengikut kumpulan tertentu: kawalan, H 2 O 2 , madu gelam, H 2 O 2 + madu gelam. Selepas 24 jam rawatan, biopetanda tekanan oksidatif seperti malondialdehid (MDA) dan protein karbonil ditentukan. Aruhan tekanan oksidatif meningkatkan aras MDA (p<0.05) tetapi tidak memberi kesan kepada protein karbonil. Rawatan madu gelam didapati menurunkan aras MDA (p<0.05) dalam sel A549 yang diaruh dengan H 2 O 2 tetapi tidak memberi kesan kepada protein karbonil. Rawatan madu gelam sahaja tidak mempunyai kesan terhadap aras MDA dan protein karbonil. Kesimpulannya, madu gelam menurunkan peroksidasi lipid tetapi bukan oksidasi protein dalam sel peparu manusia teraruh tekanan oksidatif.
Royal jelly modulates oxidative stress and tissue injury in gamma irradiated male Wister Albino rats
North American Journal of Medical Sciences, 2011
Background: Royal jelly is a nutritive secretion produced by the worker bees, rich in proteins, carbohydrates, vitamins and minerals. Aim: The present study was designed to determine the possible protective effects of royal jelly against radiation induced oxidative stress, hematological, biochemical and histological alterations in male Wister albino rats. Materials and Methods: Male Wister albino rats were exposed to a fractionated dose of gamma radiation (2 Gy every 3 days up to 8 Gy total doses). Royal jelly was administrated (g/Kg/day) by gavages 14 days before exposure to the 1 st radiation fraction and the treatment was continued for 15 days after the 1 st irradiation fraction till the end of the experiment. The rats were sacrificed 3 rd , equivalent to 3rd post 2nd irradiation fraction, and equivalent to 3rd day post last irradiation fraction. Results: In the present study, gamma-irradiation induced hematological, biochemical and histological effects in male Wister albino rats. In royal jelly treated irradiated group, there was a noticeable decrease recorded in thiobarbituric reactive substances concentration when compared to γ-irradiated group. Also, the serum nitric oxide concentration was significantly improved. The administration of royal jelly to irradiated rats according to the current experimental design significantly ameliorates the changes induced in serum lipid profile. Moreover, in royal jelly treated irradiated group, there was a noticeable amelioration recorded in all hematological parameters along the three experimental intervals. The microscopic examination of cardiac muscle of royal jelly treated irradiated rats demonstrated structural amelioration, improved nuclei and normal features of capillaries and veins in endomysium when compared to gamma-irradiated rats. Conclusion: It was suggested that the biochemical, hematological and histological amelioration observed in royal jelly (g/Kg/day) treated irradiated rats might be due to the antioxidant capacity of royal jelly active constituents.
Archive of oncology
Background: Cell damage induced by ionising radiation occurs through direct and indirect effects. Vitamin E as a natural antioxidant protects against radiation-induced cell damage. Enzymes of antioxidative defense system-superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), play the main role in endogenous cell protection from oxidative damage. The aim of this study was to examine whether a presence of a-tocopherol-acetate has the influence on activity of SOD, GSH-Px and CAT in L929 cell culture after the irradiation. Materials and methods: Cell culture L929 samples were treated with a-tocopherol acetate (10-4 M) during one hour and thereafter cells were irradiated with X-rays (25 Gy). Activity of SOD, GSH-Px and CAT was determined at 1, 24, 48, 72 and 96 hours after irradiation. Results: Irradiation in applied dose induces decrease in endogenous SOD activity but no changes in CAT and GSH-Px activity of L929 cells were observed, at 96 h after irradiation. Activity of SOD was significantly increased (p1'`0.05), CAT activity was significantly decreased (p1'`0.05), while no significant difference was found in activity of GSH-Px in irradiated and a-tocopherol acetate pre-treated L929 cells, in comaprison to irradiated but unprotected cells. Conclusion: A treatment of L929 cells with a-tocopherol acetate before irradiation protects SOD, has no effect on GSH-Px and induces decrease of CAT activity after 96 hours of irradiation. As radiation exposure itself results in changes of antioxidant enzyme activities, observed changes in the presence of a-tocopherol acetate were discussed with respect to proper balance of antioxidative defense required for radioprotection.
Brazilian Journal of Pharmaceutical Sciences, 2020
Radiotherapy is often used for the treatment of cancer. However, it causes some side effects in patients. This study aimed to determine the hepatoprotective effects of Urtica dioica L. seed-extract (UDSE) in radiation-induced liver injury. Thirty-two male rats were randomly divided into 4 groups (n=8): control(C) group: no action was taken; radiation (R) group: irradiation was administrated at 5Gy singlefraction, radiation with UDSE(R+UDSE) group: irradiation was administrated at 5 Gy single-fraction and animals were fed pellets with 30 mL UDSE/kg; UDSE group: animals were fed pellets with 30 mL UDSE/kg. All of the experiments were performed in all of the groups over 10 days. Malondialdehyde (MDA) and reduced-glutathione (GSH) levels and superoxide-dismutase (SOD), catalase (CAT), glutathione-peroxidase (GSH-Px), aspartate-transaminase (AST), and alanine-aminotransferase (ALT) activities were determined. Histopathological findings were also evaluated in liver tissues. SOD, CAT and GSH-Px activities and GSH levels in the serum and liver were significantly increased, while MDA levels decreased in the R+UDSE group compared with the R group (P<0.05). Moreover, AST and ALT serum activities in the R+UDSE group were lower than those in the R group (P<0.05). In addition, radiation induced degenerative/necrotic changes in the R group were significantly compensated in the R+UDSE group. The results showed that radiation increased oxidative stress and decreased antioxidant capacity, as well as degeneration in the liver. However, UDSE attenuated these degenerative changes.
Proceedings of the National Academy of Sciences, 1997
The involvement of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase in radiobiological processes has been described at the enzyme activity level. We irradiated radiationresistant (RR) and radiation-sensitive (RS) mice and studied antioxidant enzymes at the transcriptional and activity level. In addition, aromatic hydroxylation and lipid peroxidation parameters were determined to study radiation resistance at the oxidation level. RS BALB͞c͞J Him mice and RR C3H He͞Him mice were whole-body-irradiated with x-rays at 2, 4, and 6 Gy and killed 5, 15, and 30 min after irradiation. mRNA was isolated from liver and hybridized with probes for antioxidant enzymes and -actin as a housekeeping gene control. Antioxidant enzyme activities were determined by standard assays. Parameters for aromatic hydroxylation (o-tyrosine) and lipid peroxidation (malondialdehyde) were determined by HPLC methods. Antioxidant transcription was unchanged in contrast to antioxidant activities; SOD and CAT activities were elevated within 15 min in RR animals but not in RS mice, at all doses studied. Glutathione peroxidase activity was not different between RR and RS mice and was only moderately elevated after irradiation. No significant differences were found between RR and RS animals at the oxidation level, although a radiation dose-dependent increase of oxidation products was detected in both groups. We found that ionizing irradiation led to increased antioxidant activity only minutes after irradiation in the absence of increased transcription of these antioxidant enzymes. RR animals show higher antioxidant enzyme activities than do RS mice, but oxidation products are comparable in RS and RR mice. As unchanged transcription of antioxidant enzymes could not have been responsible for the increased antioxidant enzyme activities, preformed antioxidant enzymes should have been released by the irradiation process. This would be in agreement with previous studies of preformed, stored SOD. The finding of higher SOD and CAT activities in RR than in RS animals could point to a role for these antioxidant enzymes for the process of radiation sensitivity.
Effect of radiation hygienization of honey on its health protective properties
Food Bioscience, 2014
Effect of radiation hygienization of honey on its health protective properties in terms of antimutagenicity, anti-cancer and DNA protective properties is reported in this study. Similar to non-irradiated honey, irradiated one too reduced ethyl methanesulfonate induced mutagenicity in Escherichia coli cells by 8080% when analyzed by rifampicin resistance assay, but did not induce mutagenesis on its own. Honey displayed strong anti-proliferative property against different cancer cell lines (myeloid leukemia, breast and lung cancer) but did not affect normal cell line (Int-407, intestinal epithelial cell) indicating its differential and selective cytotoxicity. The efficacy of protection against radiation induced oxidative damage was evaluated for plasmid DNA and also bacterial cells. Honey displayed protection to supercoiled form of plasmid DNA at comparatively lower dose of 0.15 kGy and reduced the extent of strand breaks at a higher dose of 1 kGy. Besides, honey also protected the functionality of plasmid DNA which was scored in terms of its transformation efficiency. Honey too protected E. coli cells from the radiation induced oxidative damage and D 10 in the presence of honey increased by 805 fold (0.1 to 0.49 kGy). Current findings thus indicate the excellent health protective properties of honey and its potential usefulness as a dietary supplement, and also endorse radiation treatment to ensure its safety while maintaining its nutraceutical value.
The effect of Malaysian honey and its major components on the proliferation of cultured fibroblasts
Background/aim: To examine, for the first time, the effect of a selected Malaysian honey and its major components on the proliferation of cultured fibroblasts. Materials and methods: Honey and some of its components, which include the sugars, the proteins, the hydrogen peroxide produced, and the phenolics, were exposed to cultured fibroblasts. The MTT colorimetric assay was used to assess cell viability and proliferation. Results: The stimulatory effect of honey on fibroblast proliferation was observed to be time- and dose-dependent. The continuous production of hydrogen peroxide by the honey-glucose oxidase system also acts to stimulate cell proliferation in a time- and dose-dependent manner. The presence of phenolics with antioxidant properties, on the other hand, renders protection to the cells against the toxic effect of hydrogen peroxide. However, the presence of a growth factor-like substance in honey could not be ascertained. Conclusion: For the first time, honey and its major components were shown to exert stimulatory effects on cultured fibroblasts. Honey is therefore potentially useful in medicinal practices.
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