Streptococcus cuniculi sp. nov., isolated from the respiratory tract of wild rabbits (original) (raw)
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International Journal of Systematic and Evolutionary Microbiology, 2015
Four isolates of an unknown Gram-stain-positive, catalase-negative coccus-shaped organism, isolated from the pharynx of four wild rabbits, were characterized by phenotypic and molecular genetic methods. The micro-organisms were tentatively assigned to the genus Streptococcus based on cellular morphological and biochemical criteria, although the organisms did not appear to correspond to any species with a validly published name. Comparative 16S rRNA gene sequencing confirmed their identification as members of the genus Streptococcus, being most closely related phylogenetically to Streptococcus porcorum 682-03T (96.9 % 16S rRNA gene sequence similarity). Analysis of rpoB and sodA gene sequences showed divergence values between the novel species and S. porcorum 682-03T (the closest phylogenetic relative determined from 16S rRNA gene sequences) of 18.1 and 23.9 %, respectively. The novel bacterial isolate could be distinguished from the type strain of S. porcorum by several biochemica...
Streptococcus merionis sp. nov., isolated from Mongolian jirds (Meriones unguiculatus)
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2009
Gram-positive, catalase-negative, chain-forming, coccus-shaped organisms were isolated both from intraperitoneally grown vesicles of the fox tapeworm Echinococcus multilocularis and the oropharynges of laboratory-kept Mongolian jirds (Meriones unguiculatus). The strains displayed no haemolytic activity on Columbia sheep blood agar, pyrrolidonyl arylamidase activity was negative and the organisms reacted weakly with Lancefield group D antiserum. On the basis of phenotypic characteristics, the strains were tentatively identified as members of the genus Streptococcus. Comparative 16S rRNA gene sequencing studies confirmed their assignment to the genus Streptococcus and revealed that Streptococcus hyointestinalis DSM 20770 T was their closest phylogenetic neighbour (96.5 % sequence similarity). The levels of 16S rRNA gene sequence similarity between the isolates and representatives of species of the genus Streptococcus were only 95.7-96.2 %. On the basis of the phenotypic and molecular data presented, the isolates from Mongolian jirds represent a novel species of the genus Streptococcus, for which the name Streptococcus merionis sp. nov. is proposed. The type strain is WUE3771 T (5DSM 19192 T 5CCUG 54871 T ).
Identification of Major Streptococcal Species by rrn-Amplified Ribosomal DNA Restriction Analysis
Journal of Clinical Microbiology, 2003
Amplified ribosomal DNA restriction analysis (rrn-ARDRA) is based on PCR amplification and restriction of a fragment of rRNA genes including 16S and 23S genes and the intergenic spacer. rrn-ARDRA was evaluated for the identification of species within the genus Streptococcus. A total of 148 type and reference strains of pyogenic, oral, and group D streptococci were examined in order to construct a database for identification of streptococci. The amplified product was a single band approximately 4,500 bp long. This amplicon was digested separately with three (HhaI, MboII, and Sau3A) restriction endonucleases. Respectively, 27, 26, and 28 major patterns were observed after HhaI, MboII, and Sau3A restrictions. Streptococcal strains belonging to different species had different patterns or different combination of patterns. An identification system based upon a combination of the three restriction patterns in a single database was then proposed. rrn-ARDRA was successfully applied to 11 clinical isolates whose identification to the species level was difficult to obtain by phenotypic analysis. Using a database of well-characterized strains, rrn-ARDRA is a powerful method for the identification of streptococcal isolates.
Microbiology-sgm, 2003
The 16S-23S rRNA internal transcribed spacer (ITS) region of several Streptococcus thermophilus strains and some related dairy streptococci, S. macedonicus, S. salivarius and S. bovis, was analysed by sequence analysis. All the Streptococcus species were easily discriminated on the basis of sequence variations principally located upstream and downstream of the region encompassing the double-stranded processing sites and the tRNA Ala gene. Comparison between tRNA Ala gene sequences highlighted a high level of sequence conservation among the Streptococcus species investigated despite their belonging to separated phylogenetic clusters, i.e. the S. salivarius and S. bovis rRNA groups. A low but significant degree of variability was detected among the S. thermophilus strains, allowing the identification of four different ITS sequences. Similarity analysis of the ITS sequences showed that the Streptococcus species were clustered in two main branches, one containing S. macedonicus and S. bovis strains, and one containing S. thermophilus and S. salivarius strains. With the aim of developing a rapid tool for the identification of the dairy streptococci species a multiplex ITS-SSCP analysis of two discrete regions within the ITS locus was carried out.
J. Clin. Microbiol.-2001-Hassan-1618-21 PCR detection
Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis.
Streptococcus porci sp. nov., isolated from swine sources
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2010
Two unidentified Gram-positive, catalase-negative, coccus-shaped organisms were recovered from pigs and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Streptococcus, although the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequence studies confirmed this identification and showed that the nearest phylogenetic relatives of the unknown cocci were Streptococcus plurextorum 1956-02T and Streptococcus suis NCTC 10234T (97.9 and 96.0 % 16S rRNA gene sequence similarity, respectively). The new isolates were related most closely to S. suis CIP 103217T based on rpoB gene sequence analysis (<8 % sequence divergence). DNA–DNA pairing studies showed that one of the unidentified strains (2923-03T) displayed DNA relatedness values of 26.6 and 27.2 % with S. plurextorum CECT 7308T and S. suis NCTC 10234T, respectively. On the basis of phenotyp...
International Journal of Systematic and Evolutionary Microbiology, 1998
The 165 rRNA gene sequences of reference strains of Streptococcus suis serotypes 1-34 and 1/2 were determined. A comparative sequence analysis showed that the degree of sequence similarity between 5. suis reference strains ranged from 93-94 to 100°/~. A dendrogram was constructed from the similarity matrix. Thirty-two strains representing 32 serotypes fell into a major group divided into three clusters. The other strains, 5. suis serotypes 32,33 and 34, were more distant. Biochemical characterization of the six more distant strains, including 5. suis serotypes 20,22, 26, 32, 33 and 34, revealed a profile similar to that of other 5. suis serotypes. Comparison of the 165 rRNA gene sequences of 5. suis reference strains with sequences of other members of the genus Streptococcus indicated that, with the exception of 5. suis serotypes 32, 33 and 34, reference strains did not cluster with any other species in the genus. In conclusion, 165 rRNA gene sequence analysis defined a major group of 5. suis reference strains which were very closely related and a higher divergence for 5. suis serotypes 32,33 and 34. However, to date, there is no strong evidence to reclassify strains of these serotypes in another species.
Streptomyces burgazadensis sp. nov., isolated from soil
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2014
A new actinobacterial strain, designated Z1R7 T , was isolated from a soil sample collected from Burgazada, in the Marmara Sea (Turkey), and the strain identity was determined using a polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Streptomyces and formed a distinct phyletic line in the 16S rRNA gene tree, together with the type strains of Streptomyces specialis GW 41-1564 T (95.76 %), Streptomyces mayteni YIM 60475 T (95.64 %), Streptomyces hainanensis YIM 47672 T (95.53 %), Streptomyces hoynatensis S1412 T (95.29 %), Streptomyces avicenniae MCCC 1A01535 T (94.74 %), Streptomyces sedi YIM 65188 T (94.59 %) and Streptomyces zhaozhouensis NEAU-LZS-5 T (94.68 %), respectively. Chomotaxonomic data revealed that strain Z1R7 T posseses MK-9(H 8 ) as predominant menaquinone, LL-diaminopimelic acid as the diagnostic diamino acid, and whole cell sugars galactose, glucose and ribose, diphosphatidylglycerol, phoshphatidylethanolamine and phosphatidylinositol as predominant polar lipids, iso-C 16:0 , anteiso-C 17:0 and anteiso-C 15:0 are major fatty acids, and the genomic DNA G+C content was 69.4 mol %. On the basis of these
Annals of Clinical Microbiology and Antimicrobials, 2011
Background: Streptococcus is an economically important genus as a number of species belonging to this genus are human and animal pathogens. The genus has been divided into different groups based on 16S rRNA gene sequence similarity. The variability observed among the members of these groups is low and it is difficult to distinguish them. The present study was taken up to explore 16S rRNA gene sequence to develop methods that can be used for preliminary identification and can supplement the existing methods for identification of clinicallyrelevant isolates of the genus Streptococcus. Methods: 16S rRNA gene sequences belonging to the isolates of S. dysgalactiae, S. equi, S. pyogenes, S. agalactiae, S. bovis, S. gallolyticus, S. mutans, S. sobrinus, S. mitis, S. pneumoniae, S. thermophilus and S. anginosus were analyzed with the purpose to define genetic variability within each species to generate a phylogenetic framework, to identify species-specific signatures and in-silico restriction enzyme analysis. Results: The framework based analysis was used to segregate Streptococcus spp. previously identified upto genus level. This segregation was validated using species-specific signatures and in-silico restriction enzyme analysis. 43 uncharacterized Streptococcus spp. could be identified using this approach.