Microbiologic Analysis Of Teeth With Failed Endodontic Treatment And The Outcome Of Conservative Re-treatment (original) (raw)
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Microbiological status of root canal after unsuccessful endodontic treatment
Serbian Dental Journal
Summary Introduction The main objective of endodontic treatment is to eliminate infection from root canal and prevent reinfection by three-dimensional hermetic obturation of the canal system. Endodontic failure can occur due to inability of complete control and elimination of infection from the root canal. The aim of this study is to investigate, by PCR technique, microbiological status of previously endodontically unsuccessfully treated teeth immediately after the removal of obturation material. Material and Methods The analysis included 30 teeth indicated for endodontic retreatment. After removing previous root canal filling material, the bacteriological sample was taken by sterile instrument (# 15) and paper points. Standard PCR technique was used to analyze the incidence of E.faecalis, P.micros, P.intermedia, P. endodontalis and A.actinomycetemcomitans. Results Positive bacteriological findings were registered in 80% of cases, while bacteria were not identified in 20% of all sam...
Microbial Ecosystem Analysis in Root Canal Infections Refractory to Endodontic Treatment
Journal of Endodontics, 2016
Introduction: The purpose of this study was to combine multiple displacement amplification and checkerboard DNA-DNA hybridization to qualitatively and quantitatively evaluate the microbiota present in infections refractory to endodontic treatment. Methods: The subjects of this study were 40 patients presenting with periapical lesions refractory to endodontic treatment. Samples were taken by scraping or filing root canal walls with a #10 K-type hand file. Sample DNA was amplified by multiple displacement amplification, and the levels of 107 bacterial taxa were analyzed by checkerboard DNA-DNA hybridization. The taxa were divided into 3 distinct microbial populations depending on their mean proportion in samples (% DNA probe counts AE standard error of the mean) as follows: dominant ($3.0%), subdominant (>1.6%-3.0%), and residual (#1.6%) populations. The significance of differences was determined using the Mann-Whitney test. Results: The taxa present with the highest mean proportions (constituting the dominant population) were Corynebacterium diphtheriae (8.03 AE 0.98), Porphyromonas gingivalis (5.42 AE 2.09), Streptococcus sobrinus (5.33 AE 0.69), and Stenotrophomonas maltophilia (4.72 AE 1.73). Among the subdominant population were Eubacterium saphenum (3.85 AE 1.06), Helicobacter pylori (3.16 AE 0.62), Dialister pneumosintes (3.12 AE 1.1), Clostridium difficile (2.74 AE 0.41), Enterobacter agglomerans (2.64 AE 0.54), Salmonella enterica (2.51 AE 0.52), Mobiluncus mulieris (2.44 AE 0.6), and Klebsiella oxytoca (2.32 AE 0.66). In the population of bacteria present at the lowest mean proportions (the residual population), Bacteroides ureolyticus (0.04 AE 0.01), Haemophilus influenzae (0.04 AE 0.02), and Prevotella oris (0.01 AE 0.01) were found at the lowest mean proportions. Enterococcus faecalis was detected in the residual population (0.52 AE 0.26). Conclusions: The microbial climax community in teeth refractory to endodontic treatment not only harbors medically important species but also contains distinct microbial consortia present with different population levels.
Microorganisms from canals of root-filled teeth with periapical lesions
International Endodontic Journal, 2003
Pinheiro ET, Gomes BPFA, Ferraz CCR, Sousa ELR, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. International Endodontic Journal, 36, 1^11, 2003. Aim The objective of the present study was to identify the microbial £ora within root canals of teeth with failed root-canal treatment and to determine the association of the various species with clinical features. Methodology Sixty root-¢lled teeth with persisting periapical lesions were selected for this study. During nonsurgical endodontic re-treatment, the root-¢lling material was removed and the canals were sampled. Microbial sampling, isolation and species determination were performed using advanced microbiological techniques for anaerobic species. The association of microbiological ¢ndings with clinical features was investigated.
Microbial Flora of Root Canals of Pulpally-infected Teeth: Enterococcus faecalis a Prevalent Species
Journal of dental research, dental clinics, dental prospects, 2009
The aim of this study was to determine the microorganisms prevalent in the necrotic dental pulp and root canals of unsuccessfully treated teeth. The present study was conducted on 150 single-rooted teeth of patients referring to a dental clinic. Sampling was performed by placing a sterile paper point in the canal for 60 s. Bacterial samples were evaluated by a microbiological technique specific for anaerobic species, used for isolation and identification of sampled strains. From the 150 samples taken, 101 were from necrotic pulps (primary infection) and 49 were from the teeth with an unsuccessful endodontic treatment (secondary infection). Enterococcus faecalis was a prevalent species in the failed root canals evaluated.
Investigation of microorganisms in infected dental root canals
Biotechnology and …, 2006
Objective: The purpose of this study is to investigate the type of microorganisms isolated from necrotic pulp tissues and from failed endodontic treatments in infected root canals. Methods: This study is based on an experiment conducted on 90 patients between November, 2002 and November, 2003 using a sampling. One hundred single root canals were microbiologically sampled from these patients by using sterile paper points. Among 100 canals sampled, 61 had primary infection and 39 had a history of secondary infection. Microorganisms were isolated and identified by using established advanced microbiologic techniques for anaerobic species. Results: A total of 197 cultivable isolates were recovered, the microbial findings in both types of canals, such as the number of species isolated, the 68.0% of Gram-positives and 27.9% gram-negatives, the 52.8% of facultative and 43.1 % strict anaerobic microorganisms 4.1% fungi and so on. Ten canals presented no microbial growth. Conclusions: The prevalence of bacteria and fungi found in 100 root canals is the prevalence of the microbial genera in primary and secondary endodontic infection. Peptostreptococcus spp was the most predominantly isolated microbial genera,
Inspection of the Microbiota in Endodontic Lesions
Dentistry Journal
The primary objective of endodontic therapy is to create a biologically acceptable environment within the root canal system that allows for the healing and maintenance of the health of the peri-radicular tissue. Bacteria are one of the main causes of pulp problems, and they have different methods of penetrating and invading the endodontic space such as through carious lesions, traumatic pulp exposures, and fractures. The types of bacteria found range from facultative anaerobes to aerobes, up to the most resistant species able to survive in nutrient-free environments; the bacterial species Enterococcus faecalis belongs to this last group. Enterococcus faecalis is considered one of the main causes of recurring apical periodontal lesions following endodontic treatment, with persistent lesions occurring even after re-treatment. The review presented in this paper was performed in accordance with the PRISMA protocol and covers articles from the related scientific literature that were sour...
Acta odontologica Scandinavica, 2017
This study investigated the presence of Enterococcus faecalis in primary teeth with primary root canal infections and related to the possible failure of pulpectomy outcome after 36 months. Root canal samples were obtained from 25 out of 244 patients using the sterile paper cone method. The identification of E. faecalis was done with culture and molecular tests using species-specific 16S rRNA gene-based polymerase chain reaction (PCR). After 36 months, the pulpectomy outcome was evaluated. Enterococcus faecalis was found in five (20%) samples, and dental caries were the cause of primary infection in all of them. Pulpectomy outcome was evaluated only in teeth that completed the entire clinical protocol and were followed up to 36 months (n = 8). From these, 75% (n = 6) were successful and 25% (n = 2) failed. E. faecalis was present in 50% of both successful and failed cases. Enterococcus faecalis was not related to the failure of endodontic treatment of primary teeth.
Journal of Endodontics, 2008
The objective of the present study was to investigate the presence of nine bacterial species in root-filled teeth associated with periapical lesions using a polymerase chain reaction analysis and to correlate these species with clinical features of the cases. DNA was extracted from 45 canal samples of root-filled teeth with periapical lesions. A PCR assay using speciesspecific primers of 16S rDNA and the downstream intergenic spacer region was used for microbial detection. Enterococcus faecalis was the most prevalent species, detected in 77.8% of the study teeth, followed by Peptostreptococcus micros, detected in 51.1%. Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, and Prevotella nigrescens were detected in 35.6%, 22.2%, 11.1%, and 11.1% of the sampled teeth, respectively. Moreover, PCR detected Filifactor alocis in 26.7%, Treponema denticola in 24.4%, and Tannerella forsythia in 4.4% of the samples. T. denticola and P. micros were statistically associated with tenderness to percussion (p Ͻ 0.05). P. nigrescens was associated with the presence of spontaneous pain and abscess (p Ͻ 0.05). P. endodontalis and P. nigrescens were associated with purulent exudates (p Ͻ 0.05). Synergistic relationship was also observed between some species. The results of this study indicated that E. faecalis was the most frequently identified test species by PCR in teeth with failing endodontic treatment. (J Endod 2008;34:537-540)