Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov., thermophilic anaerobic representatives of the phylum Planctomycetes (original) (raw)
Related papers
International Journal of Systematic and Evolutionary Microbiology, 2015
A novel thermophilic planctomycete (strain SVX8 T) was isolated from a shallow submarine hydrothermal vent, Vulcano Island, Italy. The temperature range for growth was 30-68 8C, with an optimum at 55 8C. The pH range for growth was 5.0-9.0, with an optimum at pH 7.0-8.0. Growth was observed at NaCl concentrations ranging from 0.8 to 4.5 % (w/v) with an optimum at 2.5-3.5 % (w/v). The isolate grew anaerobically using a number of mono-, di-and polysaccharides as electron donors and nitrate or elemental sulfur as electron acceptors or by fermentation. Nitrate was reduced to nitrite; sulfur was reduced to sulfide. Strain SVX8 T did not grow at atmospheric concentration of oxygen but grew microaerobically (up to 2 % oxygen in the gas phase). The G+C content of the DNA of strain SVX8 T was 58.5 mol%. Based on phylogenetic position and phenotypic features, the new isolate is considered to represent a novel species belonging to a new genus in the order Planctomycetales, for which the name Thermostilla marina gen. nov., sp. nov. is proposed. The type strain of Thermostilla marina is SVX8 T (5JCM 19992 T 5VKM B-2881 T). Strain SVX8 T is the first thermophilic planctomycete isolated from a marine environment.
Planctomycetes: a phylum of emerging interest for microbial evolution and ecology
World Federation for Culture Collections …, 2004
Planctomycetes are a group of budding, peptidoglycan-less bacteria of increasing significance for microbial evolution, ecology, cell biology and genomics. Studies of both cultured isolates and clone library sequences from natural communities have enriched this significance. Their display of unusual distinctive features such as compartmentalized cell organization, ability of some species to grow anaerobically and autotrophically via oxidation of ammonium, and the possession of large genomes combined with their wide distribution in a variety of habitats reinforces an increasing interest in them.
Antonie van Leeuwenhoek
Pan44T, a novel strain belonging to the phylum Planctomycetes, was isolated from a red biofilm in a hydrothermal area close to the island Panarea in the Tyrrhenian Sea north of Sicily, Italy. The strain forms white colonies on solid medium and displays the following characteristics: cell division by budding, formation of rosettes, presence of matrix or fimbriae and long stalks. The cell surface has an interesting and characteristic texture made up of triangles and rectangles, which leads to a pine cone-like morphology of the strain. Strain Pan44T is mesophilic (temperature optimum 26 °C), slightly alkaliphilic (pH optimum 8.0), aerobic and heterotrophic. The strain has a genome size of 6.76 Mb with a G + C content of 63.2%. Phylogenetically, the strain is a member of the family Planctomycetaceae, order Planctomycetales, class Planctomycetia. Our analysis supports delineation of strain Pan44T from all known genera in this family, hence, we propose to assign it to a novel species with...
Antonie van Leeuwenhoek, 2020
Planctomycetes are very intriguing bacteria that impassion, inspire and stimulate the scholars that study them. Discovered in the beginning of the last century, they were initially confused with 'floating fungus', hence the genus name Planctomyces (planktos, wandering, floating; Gr. masc. n. mukeˆs, fungus; https://lpsn.dsmz.de/genus/planctomyces). However, it was only in the second half of the twentieth century that their diversity started to be unveiled (Devos and Ward 2014; Lage et al. 2019; Dedysh et al. 2020a). Together with Chlamydiae, Verrucomicrobia and other poorly described phyla, such as Lentisphaerae, Kiritimatiellaeota and other candidate phyla, they form the Planctomycetes-Verrucomicrobia-Chlamydiae (PVC) superphylum (Wagner and Horn 2006).
Aquisphaera giovannonii gen. nov., sp. nov., a planctomycete isolated from a freshwater aquarium
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2011
As part of a study of the diversity of planctomycetes, two novel strains, designated OJF2 T and OJF8, were isolated from the sediments of a freshwater aquarium. The organisms were chemoheterotrophic, spherical and pink-pigmented, had an optimum growth temperature of about 30-35 6C and an optimum pH for growth of around 7.5-8.5. The predominant fatty acids were C 18 : 1 v9c and C 16 : 0 . The two strains were able to assimilate several sugars and organic acids. 16S rRNA gene sequence analysis confirmed the affiliation of these organisms to the phylum 'Planctomycetes'; they showed highest similarity to the type strains of Singulisphaera acidiphila (92.4 %) and Isosphaera pallida (91.9 %). On the basis of physiological, biochemical and chemotaxonomic characteristics, strains OJF2 T and OJF8 are considered to represent a novel species of a new genus of the order Planctomycetales, for which the name Aquisphaera giovannonii gen. nov., sp. nov. is proposed. The type strain of Aquisphaera giovannonii is OJF2 T (5CECT 7510 T 5DSM 22561 T ).
Isolation of Gemmata-Like and Isosphaera-Like Planctomycete Bacteria from Soil and Freshwater
Applied and Environmental Microbiology, 2002
New cultured strains of the planctomycete division (order Planctomycetales) of the domain Bacteria related to species in the genera Gemmata and Isosphaera were isolated from soil, freshwater, and a laboratory ampicillin solution. Phylogenetic analysis of the 16S rRNA gene from eight representative isolates showed that all the isolates were members of the planctomycete division. Six isolates clustered with Gemmata obscuriglobus and related strains, while two isolates clustered with Isosphaera pallida. A double-membrane-bounded nucleoid was observed in Gemmata-related isolates but not in Isosphaera-related isolates, consistent with the ultrastructures of existing species of each genus. Two isolates from this study represent the first planctomycetes successfully cultivated from soil.
International journal of systematic and evolutionary microbiology, 2017
A gram-negative, budding, catalase negative, oxidase positive and non-motile bacterium (MBLW1T) with a complex endomembrane system has been isolated from a freshwater lake in southeast Queensland, Australia. Phylogeny based on 16S rRNA gene sequence analysis places the strain within the family Planctomycetaceae, related to Zavarzinella formosa (93.3 %), Telmatocola sphagniphila (93.3 %) and Gemmata obscuriglobus (91.9 %). Phenotypic and chemotaxonomic analysis demonstrates considerable differences to the type strains of the related genera. MBLW1T displays modest salt tolerance and grows optimally at pH values of 7.5-8.0 and at temperatures of 32-36 °C. Transmission electron microscopy analysis demonstrates the presence of a complex endomembrane system, however, without the typically condensed nucleoid structure found in related genera. The major fatty acids are 16 : 1 ω5c, 16 : 0 and 18 : 0. Based on discriminatory results from 16S rRNA gene sequence analysis, phenotypic, biochemica...