New Globoseries Glycosphingolipids in Human Teratocarcinoma Reactive with the Monoclonal Antibody Directed to a Developmentally Regulated Antigen, Stage-Specific Embryonic Antigen 3 (original) (raw)
Related papers
American Journal Of Pathology
Monoclonal antibodies raised against and/or recognizing stage-specific antigens on preimplantation mouse embryos and stem cells of murine teratocarcinoma were used to localize these antigens immunohistochemically on human testicular germ cell tumors. SSEA-1, the antigen found on mouse embryonal carcinoma (EC) cells and embryonic cells from the 8-cell stage embryo onward, including the fetal primordial germ cells, was detected on yolk sac carcinoma components of human tumors, but not on EC cells. SSEA-3, the antigen found on follicular ova, fertilized eggs, early cleavage stage embryonic cells, and visceral endodermal cells of RECENT DEVELOPMENTS in monoclonal antibody production have considerably advanced the search for tumor-specific markers. Using the technique of Kohler and Milstein,1 several research groups claim to have identified various antigens that are found exclusively on certain animal or human tumors.2`4 The possible diagnostic application of monoclonal tumor-specific antibodies in the diagnosis of tumor makes it mandatory that all these claims be critically evaluated and independently confirmed prior to applying each technique to diagnostic human pathology. Cross-reactivity of antibodies raised against one tumor should be checked with other tumors and normal tissues of the same species.5 Since organ-specific tumors in one species often resemble equivalent tumors in other species, one should study the antigenic complementarity between histologically identical tumors from various species. Such screening may reveal antigens found in other species that could be of diagnostic value in man. In this study we used monoclonal antibodies to compare and point out the similarities and disparities between the murine and human germ cell tumors. the mouse embryo, but not on mouse EC cells, was detected on human EC cells. Both antigens were found on the cell surface of fetal testicular germ cells but not in the seminiferous tubules of adult human testes. These data point out differences between human and murine EC cells suggesting that human EC cells correspond developmentally to a less mature embryonic cell than the murine EC cells. The possible histogenesis of human germ cell tumors from primordial and/or fetal germ cells is briefly discussed. (Am J Pathol 1982, 108:225-230) In a previous report, we described a monoclonal antibody, prepared by immunizing mice with a retransplantable murine teratocarcinoma.6 This antibody binds only to murine embryonic carcinoma (EC) cells, the undifferentiated stem cells of teratocarcinoma, but not to the differentiated descendants of these tumor cells. Since the antigen recognized by this antibody appears at the 8-cell stage of murine embryonic development but is not detectable in the earliest cleavage stages nor the fertilized ovum, it was termed "stage-specific embryonic antigen" (SSEA-1). Absorption and immunohistochemical studies have shown that the same antigenic determinant exists on some fetal, embryonic, and adult tissues of mice, including the ectodermal cells of the early postimplantation stage embryo, the primordial germ cells, ejacu-
Cell Differentiation, 1985
Molecules carrying SSEA-I were isolated from [3H]galactose-labeled embryonal carcinoma cells by detergent solubilization followed by indirect immunoprecipitation. The antigenic molecules were degraded by extensive pronase digestion or mild alkaline treatment, and the majority of the products thus formed were so large as to be excluded from a column of Sephadex G-filL Therefore, the major carbohydrate constituent of the antigenic molecule was embryoglycan, the glycoprotein-bound large glyean in early embryonic cells. Furthermore, the binding of isolated embryoglycan with anti-SSEA-1 was directly shown by a modified Farr's assay. From these results we concluded that SSEA-I determinant was carried by the large glycan.
International Journal of Cancer, 1994
Glycolipids of human germ cell tumor lines were analyzed t o define the most common immunohistochemical profiles of embryonal carcinoma (EC), differentiated derivatives of EC, yolk sac carcinoma (YC) and choriocarcinoma (CC). Glycolipid composition was examined by high-performance thin-layer chromatography (HPTLC) combined with immunostaining with a panel of anti-carbohydrate monoclonal antibodies (MAbs). All EC cell lines were found t o contain high levels of globo-series glycolipids, including globotriosylceramide (Gb3), globoside (Gb4), Gb5 (GalpI -3Gb4) and GL7 (sialyl GalPI + 3Gb4).
International Journal of Cancer, 1982
A cloned human embryonal carcinoma (EC) cell line has been derived from a testicular teratocarcinoma, and r e producibly forms EC tumors when injected into athymic (nuhu) mice. These human EC cells are characterized by a newly described stage-specific embryonic antigen, SSEA-3. Unlike murine EC cells, they express major. histocompatibility antigens (HLA-A, 8, C and µglobulin) but do not express the embryonic antigen SSEA-I. We also report that these cells appear to be capable of differentiation and that this can be induced by initiating cultures at low cell density. Differentiation is marked by the appearance of morphologically distinct cells and by the induction of SSEA-I, whereas the expression of other antigens, including SSEA-3, is initially diminished. This well-characterized system of human EC cells provides a model for the future investigation of other human teratocarcinoma cell lines and for the analysis of cellular differentiation during early human development.
International Journal of Cancer, 1984
Three novel species-specific monoclonal antibodies 5.I.H, 8.7.D and 13.7.A raised against semi-purified detergent solubilized fractions of the Tera I embryonal carcinoma (EC) cell line are restricted in their in vitrodistribution to undifferentiated human EC cell lines. Competition experimenb have established that distinct antigenic specificities are seen by the 3 different monoclonal antibodies. All 3 antigens 5.I.H. 8.7.D and 13.7.A. defined by these monoclonal antibodies, undergo developmental regulation and cease to be expressed on Tera 2 clones 5 and I 2 after retinoic-acid-induced differentiation and on UCR LON HT 39/7 Celts after phorbol-ester-induced differentiation. These results taken together with the extremely limited in vivotissue distribution of the defined antigens suggest that the 5. I.H, 8.7.D and 13.7.A monoclonal antibodies define distinct onco-foetal antigens. MATERIAL AND METHODS Cell lines and culture conditions Mouse myeloma cell lines P3-NSl-AgI4 (NS1) and P3-X63-AgB (X63) were obtained from Dr. G. Kohler, Basel Institute for Immunology. The origins of the other in vitro cell lines used in these studies are shown in Table I. The human teratoma cell lines Tera 1 and Tera 2 were a kind gift from Dr.
Glycolipids of human primary testicular germ cell tumours
British journal of cancer, 1996
The glycolipid content of human non-seminomatous germ cell tumour cell lines correlates with their differentiation lineage. To analyse whether this reflects the situation in primary tumours, we studied five embryonal carcinomas, five yolk sac tumours and nine (mixed) non-seminomas, using thin-layer chromatography and carbohydrate immunostaining. We also analysed the glycolipid content of 19 seminomas to reveal their relationship with non-seminomas. Lactosylceramide (CDH) was detected in all embryonal carcinomas, but in fewer than half of the seminomas. Seminomas and embryonal carcinomas contained globoseries glycolipids, including globotriosylceramide (Gb3), globoside (Gb4), galactosy globoside (Gb5) and sialy1 galactosyl globoside (GL7). The lacto-series glycolipid Le(x) was found in all embryonal carcinomas, but only in one seminoma. Gangliosides GD3 and GT3 were detected in many seminomas, but rarely in embryonal carcinomas. Yolk sac tumours displayed a heterogeneous glycolipid p...
The American journal of pathology, 1982
Monoclonal antibodies raised against and/or recognizing stage-specific antigens on preimplantation mouse embryos and stem cells of murine teratocarcinoma were used to localize these antigens immunohistochemically on human testicular germ cell tumors. SSEA-1, the antigen found on mouse embryonal carcinoma (EC) cells and embryonic cells from the 8-cell stage embryo onward, including the fetal primordial germ cells, was detected on yolk sac carcinoma components of human tumors, but not on EC cells. SSEA-3, the antigen found on follicular ova, fertilized eggs, early cleavage stage embryonic cells, and visceral endodermal cells of the mouse embryo, but not on mouse EC cells, was detected on human EC cells. Both antigens were found on the cell surface of fetal testicular germ cells but not in the seminiferous tubules of adult human testes. These data point out differences between human and murine EC cells suggesting that human EC cells correspond developmentally to a less mature embryonic...