Separation and Analysis of Peptides and Proteins (original) (raw)

1995, Analytical Chemistry

AI-generated Abstract

This review focuses on the separation and analysis of peptides and proteins using high-performance liquid chromatography (HPLC), affinity chromatography, and capillary electrophoresis, highlighting advancements made between 1990 and 1993. The integration of mass spectrometry, particularly for on-line detection, is discussed along with developments in nuclear magnetic resonance (NMR) that provide valuable insights into protein structures. The challenges and techniques pertinent to industrial and research settings for the effective purification and characterization of bioactive compounds are examined, emphasizing the importance of optimizing chromatographic conditions.