The nonstructural proteins of the autonomous parvovirus minute virus of mice interfere with the cell cycle, inducing accumulation in G2 (original) (raw)

1995, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research

The nonstructural (NS) proteins of the autonomous parvovirus minute virus of mice (prototype strain) are involved in viral DNA replication and in the regulation of parvoviral and heterologous promoters. By constructing cell lines having integrated the NS coding sequence under the control of an inducible promoter, we were able to demonstrate that NS proteins are toxic, once expressed in the transformed cells. Cell killing appears after several days of NS expression, suggesting that NS toxicity involved cellular factors. In this paper, we show that NS proteins are cytotoxic and interfere with the cell cycle in proliferating cells only NS expression is innocuous in resting cells, whereas in growing cells, it induces the accumulation of G2 cells. This cytostatic effect is enhanced upon neoplastic transformation, which sensitized the cells to NS killing. Moreover, as clones resistant to NS toxicity undergo no alteration of their cycle, this cytostatic effect of NS proteins could be an ea...

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