Comparison of the presence of tumor cells in purged marrow versus peripheral blood in stem cell grafts for Neuroblastoma (original) (raw)

Abstract

The presence of neuroblastoma cells in marrow grafts has been linked to the recurrence of the disease post bone marrow transplantation. To avoid this problem, different techniques have been developed to purge tumor cells from the graft. We studied the presence of neuroblasts in bone marrows (BM) of neuroblastoma patients undergoing autologous bone marrow transplantation (ABMT) before and after immunomagnetic purging and compared it to their presence in peripheral blood (PBL). The presence of neuroblasts was also measured in peripheral blood stem cells (PBSC) pheresed after G-CSF stem cell mobilization. We employed the immunofluorencense (IF) method utilizing polylysine-coated dishes or immunocytochemistry (IC) using avidin-biotin peroxidase techniques combined with anti-neuroblastoma monoclonal antibodies. These techniques are capable of identifying one tumor cell in 10 5 mononuclear BM or PBL cells. To further investigate tumor contamination, we tested marrows before and after purg...

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