Prevalence of some herpesviruses in gingival crevicular fluid (original) (raw)
Related papers
Journal of Clinical Periodontology, 2008
Aims: To evaluate (i) the presence of human herpesvirus 7 (HHV-7), Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV), and (ii) the transcription pattern of HHV-7 in gingival biopsies from patients affected by periodontitis (P) and periodontally healthy subjects (H). Material and Methods: Thirty-seven subjects (P: n 5 24; H: n 5 13) were included. Each P patient contributed two gingival biopsies (representative of a clinically affected and non-affected site) and each H subject contributed one gingival biopsy. After DNA extraction, nested polymerase chain reaction was used to identify the viruses. Results: HHV-7 was detected in 91.7% of P patients and in 61.5% of H subjects (p 5 0.02), EBV in 50.0% samples of P patients and 7.7% of H subjects (p 5 0.005) and HCMV only in one sample from H group. EBV was more frequently detected in biopsies from affected sites (50.0%) than from non-affected sites (16.7%) (p 5 0.008). HHV-7 transcription was detected in 15.4% of affected and 15.4% of non-affected sites.
Association of Herpesvirus and Periodontitis: A Clinical and Laboratorial Case–Control Study
European Journal of Dentistry, 2023
Objectives A significant influence of the Herpesviridae family in the progression of periodontal disease has been suggested. The aim of this study was to investigate the potential association of four Herpesviruses (HSV-1, HSV-2, cytomegalovirus [CMV], and Epstein-Barr virus [EBV]) with periodontal disease using a qualitative test for evaluating the presence or absence of viral DNA in crevicular fluid samples of both healthy periodontal patients and periodontal compromised patients. Materials and Methods A case-control study was conducted in 100 participants at a university clinic. A qualitative test was used for evaluating the presence/absence of viral DNA in crevicular fluid samples of both healthy periodontal patients and periodontal compromised patients, and considering the periodontitis staging (stage II, stage III, and stage IV) and grading (grade A, grade B, and grade C). Statistical Analysis The distribution of the same exposure variables to the periodontitis staging and grading was compared using Chi-square, Fisher's exact, and Gamma tests depending on the variable characteristics. The significance level was set at 5%. The association of the variables: age, sex, diabetes, smoking, alcohol, and oral hygiene was also considered. Results The prevalence of Herpesviridae family virus DNA was 6% for the periodontal healthy group and 60% for the periodontitis group (roughly 60% on periodontitis stages II, III, and IV, p <0.001; and twofold increase in moderate and rapid progression grades compared with the slow progression grade, p <0.001). HSV1 DNA was prevalent in all periodontitis stages and grades. HSV 2, EBV, and CMV DNA had increasing prevalence rates in more severe stages (stages III and IV, p <0.001); while considering periodontitis grade, HSV2 (p ¼ 0.001), CMV (p ¼ 0.019) and EBV (p <0.001) DNA were prevalent only in grades B and C, with EBV DNA registering a marked prevalence in grade C. Conclusion A significant different distribution of Herpesviridae virus DNA per each stage of disease was registered.
The Journal of Contemporary Dental Practice, 2015
Objective The identification of new uncultured species and viruses supports the possibility of combination of the herpesvirus- bacterial periodontal infection for periodontitis. The paucity of data and studies with larger sample size in Indian subjects provides an unclear picture of the presence of the herpesvirus in this population. Materials and methods This was a cross-sectional study consisting of 100 each in the healthy group and chronic periodontitis (CP) group. The subgingival plaque was collected and polymerase chain reaction was performed post deoxyribonucleic acid (DNA) extraction by using specific primers for human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV). The data were analyzed using Fisher's exact test, Mann-Whitney U test and Spearman's coefficient correlation. Results Human cytomegalovirus and EBV viruses were significantly higher in the CP group as compare to the healthy group. A higher percentage of those with CMV positive had EBV also positive (2...
Herpesviruses in chronic and aggressive periodontitis patients in an Indian population
Journal of Oral Science, 2009
Many recent studies have assessed the prevalence and role of herpesviruses in the etiopathogenesis of periodontal diseases, which has led to the realization of intricate interactions between viruses and bacteria within periodontal pockets. It has also been shown that the occurrence of herpesviruses may vary depending upon the age of the patient and the race of the population studied. Thus, the present study aimed at detecting herpes simplex virus type 1 and 2 (HSV 1 and 2), Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) in periodontal pockets of Indian patients with chronic and aggressive periodontitis. Subgingival plaque samples (n = 33) were collected from 19 randomly chosen chronic periodontitis and 14 aggressive periodontitis patients. Herpesviruses were detected using multiplex polymerase chain reaction technique. Chronic periodontitis patients revealed presence of HSV-1 in 19 (100%) samples, HSV-2 in 3 (15.7%), EBV in 15 (78.9%) and HCMV in 5 (26.31%) samples. Samples from aggressive periodontitis patients showed the presence of HSV-1 in 8 (57.14%), EBV in 4 (28.57%) and HCMV in 1 (7.14%), whereas HSV-2 was not detected in any specimen. In this population, herpesviruses were found more frequently in chronic periodontitis than in aggressive periodontitis patients and their prevalence may vary according to the age and race of the patient. (J Oral Sci 51, [79][80][81][82][83][84][85][86] 2009)
Herpesviruses 6, 7 and 8 in HIV‐and non‐HIV‐associated periodontitis
Journal of …, 2000
Since the mid-1970s, numerous studies have examined the importance of speci®c microbial species in periodontal disease. Studies focused principally upon the role of bacteria and, to some extent, upon the role of fungi and parasites in periodontal disease (1). Virtually no attention was given to mammalian viruses in periodontal disease. However, ®ndings within the last 5 yr have provided initial evidence that human herpesviruses contribute to periodontal disease development (2). Herpesviruses are double-stranded DNA viruses displaying cytopathogenic and/or immunosuppressive properties. Eight human herpesvirus species are currently identi®ed, namely herpes simplex virus (type 1 and 2), varicella-zoster virus, Epstein±Barr virus (EBV) type 1 and 2, cytomegalovirus (HCMV), herpesvirus 6 (HHV-6), herpesvirus 7 (HHV-7) and herpesvirus 8 (HHV-8) (also known as Kaposi's sarcoma-associated herpesvirus). After primary infection, herpesviruses remain latent, in potentially reproductive form, within monocytes, macrophages, lymphocytes (3, 4), salivary gland tissue (5, 6) or nerve root ganglia (7). Reactivation of latent herpesviruses can take place spontaneously or be precipitated by host defense-impairing events including emotional stress, concurrent infections, fever, ultraviolet light exposure, tissue trauma, drugs and immunosuppression (3, 8). Oral disease has been attributed to each known species of human herpesviruses (2). Slots & Contreras (9) proposed a model by which periodontal active HCMV and EBV-1 infection may participate in the pathogenesis of human periodontal disease. Contreras & Slots (10) identi®ed active HCMV infection in deep periodontal sites of 4 of 11 individuals with adult or juvenile periodontitis. Also, Contreras et al. (11) detected
European Journal of Dentistry
Objective:This study aims to assess the role of herpesviruses in chronic periodontitis and their association with clinical parameters and in increasing severity. Materials and Methods: This was a prospective case–control study. Ethical approval and prior consent were taken. A subgingival plaque sample was collected from a total of 300 patients and 300 controls and processed for DNA extraction and multiplex polymerase chain reaction for detection of herpesviruses.Results: The most predominant age group affected was 41–50 followed by 31–40 years and male patients outnumbered the female patients. Herpes simplex virus (HSV)-1 (46.6%) was the most common Herpesvirus followed by HSV-2 (34.6%), Epstein–Barr viruses (EBV) (30.6%), and cytomegalovirus (CMV) (19.3%) in chronic periodontitis. Herpesviruses were significantly associated with increasing severity of the disease and had shown differences in their association with clinical parameters. Multiple herpesvirus infection was seen in pati...
OALib, 2016
Recent investigations suggest that the viruses of the family Herpesviridae are associated with periodontal disease, demonstrating a possible association with the presence of Human Cytomegalovirus (HCMV), Herpes Simplex Virus 1 (HSV-1) and Epstein Barr Virus (EBV) in periodontal tissues. Aim: To analyze the association between the presence of HSV-1, HCMV and EBV in patients with chronic or aggressive periodontitis. Methods: Crevicular fluid samples were collected from 11 patients with aggressive periodontitis (AgP), 11 with chronic periodontitis (CP) and 11 healthy control subjects (CG). The following clinical parameters were measured: plaque index (PI), bleeding on probing (BOP), probing depths (PD) and clinical attachment loss (CAL). Viral detection was performed by PCR for HSV-1, HCMV and EBV. Results: HCMV was detected in 27.3% of AgP patients but not detected in CP and healthy subjects; HSV-1 in 27.3% of AgP and CP group and in 9.1% of CG. EBV-1 was detected in 18.2% of CP patients and 9.1% of the CG. HCMV and HSV-1 coinfection was detected in 18.8% of AgP patients and 9.09% of viral coinfection between HSV-1 and VEB, within the CP group. The AgP group exhibited the higher values for all clinical parameters evaluated, respect to the CP and control group. Conclusion: The presence of HCMV may be related to periodontal disease, specifically in cases of AgP, and it may play a synergistic role with other infectious agents from the oral cavity associated with the development of periodontitis.