Hypochlorous acid-induced membrane pore formation in red blood cells (original) (raw)
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Membrane changes associated with lysis of red blood cells by hypochlorous acid
Free Radical Biology and Medicine, 1994
This study was carded out to investigate HOCl-induced lysis of human erythrocytes. Using reagent HOC1 with isolated red cells, we showed that the rate of lysis was dependent on the dose of HOC1 per red cell rather than on the concentration Of oxidant. The process was inhibited by scavengers such as methionine and taurine, but only if they were present at the time of addition of HOC1. Lysis was preceded by a decrease in cell density, a change in the deformability of the membrane as evidenced by ektacytometry, and an increase in K+-leak. Electron microscopy showed extensive disruption of the membrane. Increasing doses of HOC1 caused progressive loss of membrane thiols, but complete thiol oxidation by N-ethylmaleimide did not result in an equivalent rate of lysis. Restoration of oxidised thiols by incubation with glucose did not significantly alter the pattern of lysis. Taken together, these results suggest that thiol oxidation was not responsible for HOCl-mediated lysis. There was evidence of increasing crosslinking of membrane proteins on electrophoresis, only some of which was due to the formation of disulfides. TLC of the membrane lipids indicated that there may be formation of chlorohydrins by reaction of HOCI with the fatty acid double bonds. This reaction results in the formation of a more polar species which, if formed, would be extremely disrupting to the lipid bilayer. The results indicate that HOCl-mediated damage to the membrane proteins or to the lipid bilayer comprises an initial damaging event that sets the cells on a path toward eventual lysis.
Free Radical Biology and Medicine, 2001
Treatment of human erythrocyte membranes with active forms of chlorine (hypochlorous acid and chloramine T) resulted in a concentration-dependent inhibition of the membrane Na ϩ , K ϩ -and Mg 2ϩ -ATPases. Membrane protein thiol group oxidation was consistent with inactivation of enzymes and preceded oxidation of tryptophan residues and chloramine formation. Erythrocyte exposure to hypochlorous acid led to complex changes of cell membrane rigidity and cell morphological transformations: cell swelling, echinocyte formation, and haemolysis. The inhibition of ion pump ATPases of human erythrocyte membranes may be due to direct oxidation of essential residues of enzyme (thiol groups) and structural rearrangement of the membrane.
Oxidation of intracellular glutathione after exposure of human red blood cells to hypochlorous acid
Biochemical Journal, 1995
Exposure of human red blood cells to low doses of hypochlorous acid (HOCl) resulted in the loss of intracellular GSH. Oxidation occurred less than 2 min after the addition of HOCl, and required approx. 2.5 mol of HOCl per mol of GSH lost. Loss of GSH preceded oxidation of membrane thiols, the formation of chloramines and haemoglobin oxidation. The susceptibility of intracellular GSH to oxidation by HOCl was two-thirds that of GSH in cell lysates. These results indicate that HOCl can penetrate the red cell membrane, which provides little barrier protection for cytoplasmic components, and that GSH oxidation by HOCl may be a highly selective process. Virtually all of the GSH lost was converted into GSSG. If glucose was added to the medium, most of the GSH oxidized by low doses of HOCl was rapidly regenerated. At higher doses, recovery was less efficient. However, when HOCl was added as a slow infusion rather than in a single bolus, there was increased recovery at higher doses. This ind...
Bioelectrochemistry, 2002
Hypochlorous acid, one of the most powerful biological oxidants, is believed to be important in the pathogenesis of some diseases. The purpose of this study was to further characterise the membrane and intracellular events which resulted in HOCl-induced oxidative impairments and haemolysis of human erythrocytes and interaction of different oxidative agents, which accumulated during respiratory burst, in the process of RBS oxidation. The sequence of cellular events after red blood cell exposure to HOCl: cell morphological transformations, oxidation of cellular constituents, enzyme modifications, and haemolysis have been evaluated. It was shown that HOCl-treated cells underwent colloid-osmotic haemolysis, preceded by rapid morphological transformations and membrane structural transitions. The activation energy of the process of haemolysis (after removal of the excess of oxidative agent) was estimated to be 146 F 22 kJ/mol at temperatures above the break point of Arrhenius plot (31 -32 jC). This value corresponds to the activation energy of the process of protein denaturation. Modification of erythrocytes by HOCl inhibited membrane acetylcholinesterase (uncompetitive type of inhibition), depleted intracellular glutathione, activated intracellular glutathione peroxidase, but did not induce membrane lipid peroxidation. The presence of other oxidants, nitrite or tert-butyl hydroperoxide (t-BHP), promoted the oxidative damage induced by HOCl and led to new oxidative reactions. D
The Biochemical journal, 1998
Human red blood cells are lysed by the neutrophil-derived oxidant hypochlorous acid (HOCl), although the mechanism of lysis is unknown. Hypobromous acid (HOBr), a similarly reactive oxidant, lysed red cells approx. 10-fold faster than HOCl. Therefore we compared the effects of these oxidants on thiols, membrane lipids and proteins to determine which reactions are associated with lysis. There was no difference in the loss of reduced glutathione or membrane thiols with either oxidant, but HOBr reacted more readily with membrane lipids and proteins. Bromohydrin derivatives of phospholipids and cholesterol were seen at approx. one-tenth the level of oxidant than chlorohydrins were. However, these products were detected only with high concentrations of HOCl or HOBr, which caused instant haemolysis. Membrane protein modification occurred at much lower doses of oxidant and was more closely correlated with lysis. SDS/PAGE analysis showed that band 3, the anion transport protein, was lost at...
Hypochlorous acid inhibits glutathione S-conjugate export from human erythrocytes
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2002
It was found that the hypochlorous acid (HOCl) inhibits the active efflux of glutathione S-conjugates, 2,4-dinitrophenyl-S-glutathione (DNP-SG, c 50% = 258 F 24 AM HOCl) and bimane-S-glutathione (B-SG, c 50% = 125 F 16 AM HOCl) from human erythrocytes, oxidises intracellular reduced glutathione (the ratio [HOCl]/[GSH] oxidized = 4) and inhibits basal as well as 2,4-dinitrophenol-(DNP) and 2,4dinitrophenyl-S-glutathione (DNP-SG)-stimulated Mg 2 + -ATPase activities of erythrocyte membranes. Multidrug resistance-associated protein (MRP1) mediates the active export of glutathione S-conjugates in mammalian cells, including human erythrocytes. A direct impairment of erythrocyte membrane MRP by hypochlorous acid was shown by electrophoresis and immunoblotting (c 50% = 478 F 36 AM HOCl). The stoichiometry of the MRP/HOCl reaction was 1:1. These results demonstrate that MRP can be one of the cellular targets for the inflammatory mediator hypochlorous acid. D
International Journal of Molecular Sciences
This review discusses the formation of hypochlorous acid HOCl and the role of reactive chlorinated species (RCS), which are catalysed by the enzyme myeloperoxidase MPO, mainly located in leukocytes and which in turn contribute to cellular oxidative stress. The reactions of RCS with various organic molecules such as amines, amino acids, proteins, lipids, carbohydrates, nucleic acids, and DNA are described, and an attempt is made to explain the chemical mechanisms of the formation of the various chlorinated derivatives and the data available so far on the effects of MPO, RCS and halogenative stress. Their presence in numerous pathologies such as atherosclerosis, arthritis, neurological and renal diseases, diabetes, and obesity is reviewed and were found to be a feature of debilitating diseases.
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1999
HCl-induced lysis of mammalian erythrocytes, pretreated with DIDS, which is a specific inhibitor of the anion transport in their membranes, was markedly delayed. After acidification of a suspension of DIDS-inhibited cells, hemolysis was initiated by addition of a protonophore (Na-salicylate) at any moment chosen by will. These findings revealed that low-pH hemolysis depended on the rate of the transfer of acid equivalents into cytosole. Erythrocyte acid resistance was studied in a group of mammals and found to be inversely related to the rate of monovalent anion exchange in membranes which supported the above observations. In human erythrocytes, the critical level of cytosole acidification was found to be about pH 5.7 by measuring the acid equivalent absorbed by cells prior to hemolysis. HCl-induced hemolysis was also studied in human erythrocyte ghosts resealed with one-sixth of the initial hemoglobin content of cells. During the prelytic interval the ghosts suspended in isotonic NaCl/sucrose media shrunk, indicating an increase in ion permeability. The increase in prelytic permeability and hemolysis were strongly delayed in ghosts prepared from DIDS-treated cells, suggesting a uniform mechanism of lysing in cells and their ghosts. The prelytic increase in ion permeability was measured by the corresponding rate of ghost shrinkage and was found to be pH-dependent, with a high value below pH 3.4 and a very low one above pH 4.0. Compared to cells, the prelytic barrier impairment in ghosts had more mild character although it required greater concentration of cytosolic H. While finally complete, hemolysis of cells was strongly delayed in the presence of catalase (500^1500 U/ml) and superoxide dismutase (200^600 U/ml) in hemolytic media. In conclusion, the acid-induced hemolysis could be associated with an oxidative injury of membranes, mainly triggered by the entry of acid equivalents into the cytosole.
Living with a Killer: The Effects of Hypochlorous Acid on Mammalian Cells
Iubmb Life, 2001
The production of hypochlorous acid (HOCl) by the myeloperoxidase-H 2 O 2-Cl ¡ system of phagocytes plays a vital role in the ability of these cells to kill a wide range of pathogens. However, the generation of a potent oxidant is not without risk to the host, and there is evidence that HOCl contributes to the tissue injury associated with in ammation. In this review, we discuss the biological reactivity of HOCl, and detail what is known of how it interacts with mammalian cells. The outcome of exposure is dependent on the dose of oxidant, with higher doses causing necrosis, and apoptosis or growth arrest occurring with lower amounts. Glutathione (GSH) and protein thiols are easily oxidized, and are preferred targets with low, sublethal amounts of HOCl. Thiol enzymes vary in their sensitivity to HOCl, with glyceraldehyde-3-phosphate dehydrogenase being most susceptible. Indeed, loss of activity occurred before GSH oxidation. The products of these reactions and the ability of cells to regenerate oxidized thiols are discussed. Recent reports have indicated that HOCl can activate cell signaling pathways, and these studies may provide important information on the role of this oxidant in in ammation.