Mercury and selenium concentrations in skeletal muscle, liver and regionsof the heart and kidney in bearded seals from Alaska (original) (raw)
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Environmental Toxicology and Chemistry, 2015
Mean concentrations of total mercury ([THg]) and selenium ([TSe]) (mass and molar-based) were determined for 5 regions of the heart and 2 regions of the kidney of bearded seals (Erignathus barbatus) harvested in Alaska, USA, in 2010 and 2011. Mean [THg] and [TSe] of bearded seal liver and skeletal muscle tissues were used for intertissular comparison. The Se:Hg molar ratios were used to investigate elemental associations and potential antioxidant protection against Hg toxicosis. Age was an important factor in [THg] and Se:Hg molar ratios in heart and kidney. Small but statistically significant differences in mean [THg] occurred among some of the 5 heart regions (p < 0.05). Mean [THg] was highest in liver, 3.057 mg/g, and lowest in heart left ventricle, 0.017 mg/g. Mean [THg] ranked: liver > kidney cortex > kidney medulla > skeletal muscle > heart left ventricle (p < 0.001). Mean [TSe] was highest in liver, 3.848 mg/g, and lowest in heart left ventricle, 0.632 mg/g. Mean [TSe] ranked: liver > kidney cortex > kidney medulla > skeletal muscle > heart left ventricle (p < 0.001). The Se:Hg molar ratios were significantly greater than 1.0 in all tissues (p < 0.001) and represented baselines for normal [TSe] under relatively low [THg]. Mean Se:Hg molar ratios ranked: heart left ventricle > kidney medulla > kidney cortex (p < 0.001).
Total mercury and selenium concentrations ([THg], [Se]) in serum, plasma, whole blood, and packed cells were examined in a resident population of free-ranging bottlenose dolphins (Tursiops truncatus) from Sarasota Bay, Florida, USA. The authors determined how these elements partition in blood and assess compartment-specific associations. Determining the distribution of Se and THg can provide physiologic insight into potential association of Hg with selenol-containing biomolecules (e.g., antioxidants) in blood compartments. Concentrations of THg were ranked serum < plasma < whole blood < packed cells; whereas for Se concentrations, plasma < serum < whole blood < packed cells. The Se:THg molar ratio was greater than 1 in all compartments, with the higher ratios found in serum and plasma (plasma < serum) and the lower in whole blood and packed cells (packed cells < whole blood). Age was positively correlated with [THg] in all blood compartments and with [Se] in serum, plasma, and whole blood. Age was negatively correlated with Se:THg molar ratios in all blood compartments, driven by low [THg] in young animals. Although [THg] was highly correlated among all blood compartments, this was not the case for [Se]. The feasibility of calculating packed cell [THg], [Se], and Se:THg molar ratios using hematocrit measurements in combination with whole blood and plasma [THg] and [Se] was validated, allowing routine assessment of compartmentalization within erythrocytes using standard clinical measurements. Environ Toxicol Chem 2013;32:xx-xx. # 2013 SETAC
Hg and Se were measured in internal tissues of false killer whale stranded in Southern South America. Bioaccumulation of Hg and Se was found in tissues. Se-to-Hg correlations in tissues suggest the formation of mercury-selenium complexes. Hg values exceeded in all specimens the toxic thresholds for hepatic damage in marine mammals. Molar Se:Hg ratio in liver, lung and muscle were <1 indicating a possible toxicological risk of Hg. a b s t r a c t The study was carried out to determine Hg and Se concentrations in false killer whales stranded on the Estrecho de Magallanes, Chile, South America. Tissue samples of five mature specimens were analyzed (two females and three males). Mean Hg concentration in liver 1068 (234) mg g À1 dry weight (DW) (standard deviation in parenthesis) was markedly higher than those in kidney 272 (152) mg g À1 DW, lung 423 (325) mg g À1 DW, spleen 725 (696) mg g À1 DW, muscle 118 (94) mg g À1 DW and testicle 18.0 (2.8) mg g À1 DW. Mean Se concentration in liver, 398 (75) mg g À1 DW, was higher than those in kidney 162 (69) mg g À1 DW, lung 128 (84) mg g À1 DW, spleen 268 (245) mg g À1 DW, muscle 47 (38) mg g À1 DW and testicle 25.4 (2.1) mg g À1 DW. Positive correlations were found between Hg and Se molar concentrations in muscle, lung, spleen and kidney. Molar ratio of Se/Hg in liver, lung and muscle were <1, but those in kidney and testicle were markedly >1 suggesting a Se protection against Hg toxicity. In all the examined specimens Hg values exceeded the toxic thresholds defined for hepatic damage in marine mammals, with Se/Hg molar ratios below 1 implying limited protective action of Se. Generally, our results showed
Environmental Research, 2011
This study quantified the Hg levels in the liver (n ¼ 98) and heart (n ¼ 43) tissues of Harbor Seals (Phoca vitulina) (n ¼ 102) harvested from Prince William Sound and Kodiak Island Alaska. Mercury tissue dry weight (dw) concentrations in the liver ranged from 1.7 to 393 ppm dw, and in the heart from 0.19 to 4.99 ppm dw. Results of this study indicate liver and heart tissues' Hg ppm dw concentrations significantly increase with age. Male Harbor Seals bioaccumulated Hg in both their liver and heart tissues at a significantly faster rate than females. The liver Hg bioaccumulation rates between the harvest locations Kodiak Island and Prince William Sound were not found to be significantly different. On adsorption Hg is transported throughout the Harbor Seal's body with the partition coefficient higher for the liver than the heart. No significant differences in the bio-distribution (liver:heart Hg ppm dw ratios (n ¼ 38)) values were found with respect to either age, sex or geographic harvest location. In this study the age at which Hg liver and heart bioaccumulation levels become significantly distinct in male and female Harbor Seals were identified through a Tukey's analysis. Of notably concern to human health was a male Harbor Seal's liver tissue harvested from Kodiak Island region. Mercury accumulation in this sample tissue was determined through a Q-test to be an outlier, having far higher Hg concentrarion (liver 392 Hg ppm dw) than the general population sampled.
Science of The Total Environment, 2015
The mercury (Hg) level in the marine environment has tripled in recent decades, becoming a great concern because of its high toxic potential. This study reports Hg and selenium (Se) status, and the first Se/Hg molar ratio assessment in bottlenose dolphins (Tursiops truncatus) inhabiting the waters of the Canary Islands. Total Hg and Se concentrations were determined in the blubber and liver collected from 30 specimens stranded along the coasts of the archipelago from 1997 to 2013. The median values for total Hg in the blubber and liver were 80.83 and 223.77 μg g −1 dry weight (dw), and the median levels for Se in both tissues were 7.29 and 68.63 μg g −1 dw, respectively. Hg concentrations in the liver were lower than 100 μg g −1 wet weight (ww), comparable to those obtained in bottlenose dolphins from the North Sea, the Western Atlantic Ocean and several locations in the Pacific Ocean. The Mediterranean Sea and South of Australia are the most contaminated areas for both elements in this cetacean species. In addition, it must be stressed that the levels of Hg and Se in the liver showed an increasing trend with the age of the animals. As expected, a strong positive correlation between Hg and Se was observed (r s = 0.960). Surprisingly, both younger and older specimens had a Se/Hg molar ratio different from 1, suggesting that these individuals may be at greater toxicological risk for high concentrations of both elements or a deficiency of Se without a protective action against Hg toxicity.
Science of The Total Environment, 2013
A number of contaminants affect fish health, including mercury and selenium, and the selenium: mercury molar ratio. Recently the protective effects of selenium on methylmercury toxicity have been publicized, particularly for consumption of saltwater fish. Yet the relative ameliorating effects of selenium on toxicity within fish have not been examined, nor has the molar ratio in different tissues, (i.e. brain). We examined mercury and selenium levels in brain, kidney, liver, red and white muscle, and skin and scales in bluefish (Pomatomus saltatrix) from New Jersey to determine whether there were toxic levels of either metal, and we computed the selenium: mercury molar ratios by tissues. Total mercury averaged 0.32 ± 0.02 ppm wet weight in edible muscle and 0.09 ± 0.01 ppm in brain. Selenium concentration averaged 0.37 ± 0.03 in muscle and 0.36 ± 0.03 ppm in brain. There were significant differences in levels of mercury, selenium, and selenium: mercury molar ratios, among tissues. Mercury and selenium levels were correlated in kidney and skin/scales. Mercury levels were highest in kidney, intermediate in muscle and liver, and lowest in brain and skin/scales; selenium levels were also highest in kidney, intermediate in liver, and were an order of magnitude lower in the white muscle and brain. Mercury levels in muscle, kidney and skin/scales were positively correlated with fish size (length). Selenium levels in muscle, kidney and liver were positively correlated with fish length, but in brain; selenium levels were negatively correlated with fish length. The selenium: mercury molar ratio was negatively correlated with fish length for white muscle, liver, kidney, and brain, particularly for fish over 50 cm in length, suggesting that older fish experience less protective advantages of selenium against mercury toxicity than smaller fish, and that consumers of bluefish similarly receive less advantage from eating larger fish.
Relationship between erythrocyte mercury and selenium in erythrocyte, plasma and urine
Nutrition Research, 1985
Selenium in erythrocytes(ESe), plasma(PSe), and urine (USe), as well as mercury in erythrocytes(EHg), were measured for 48 normal Japanese adults. No sex-related differences were found in EHg, PSe and USe, but women had higher ESe values than men. EHg was significantly correlated with PSe in the total population and in each sex, while significant correlation was found between EHg and ESe in the total population and in men. These findings suggest that dietary fish may be a primary source of Se and Hg in the blood of normal Japanese, with a possible sex-related difference in uptake of Se into erythrocytes from plasma.