BIOSYNTHESIS, CHARACTERIZATION AND APPLICATION OF TIO2 NANOPARTICLES IN BIOCATALYSIS AND PROTEIN FOLDING (original) (raw)

The nano-TiO2 was synthesized using Lactobacillus sp. and characterized by XRD and TEM.The X-ray diffraction showed that TiO2 nanoparticles were crystalline in nature. TEM images revealed that these particles are irregular in shape with an average particle size of 50–100 nm. The biosynthesized nanoparticles were used for the immobilization and refolding of thermally inactivated alpha amylase enzyme. The enzyme after adsorption on TiO2 nanoparticles retained 71% of enzyme activity. The immobilized enzyme was found to be thermally more stable as compared to the free enzyme. When the enzyme was heated to 60°C for 60 min the free enzyme loses all of its activity whereas the adsorbed enzyme retained 82% of its activity.The adsorbed/immobilized could be reused five times without any loss in enzyme activity.The operational stability data also shows that after immobilization the stability of the alpha amylase increases.To study the nanoparticles-protein interaction, alpha amylase enzyme was...