Phytochemical investigation and in vitro antioxidant activity of Eupatorium triplinerve (original) (raw)

Objective: To investigate antioxidant potential of ethyl acetate extract of Eupatorium triplinerve Vahl Methods: The study was done by using various In vitro methods such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), Nitric oxide, Superoxide anion radical scavenging, Hydrogen peroxide radical scavenging, Iron chelating, Ferric reducing antioxidant power, (FRAP) and Total antioxidant activity. Phytochemical constituents, total phenolic content and total flavonoid content of the extract at different concentrations (50,100,150,200 and 250 μg/mL) were determined. Results: Eupatorium triplinerve Vahl leaves showed high free radical scavenging activity as evidenced by the low IC50values in DPPH (116.7 μg/mL), Superoxide anion(165.0 μg/mL), ABTS (326.0μg/mL), Nitric oxide(172.1μg/mL), H2O2(153.7μg/mL),Iron chelating (208.4μg/mL) FRAP(265.0μg/mL) and Total antioxidant activity(226.0μg/mL). Furthermore the TPC (37.48) and TFC (44.28) of the extract were found to be 40.77 Gallic acid equivalent per gram of extract and 48.56 mg quercetin equivalent per gram of extract respectively. Conclusions: The results of present comprehensive analysis demonstrated that Eupatorium triplinerve Vahl leaves possess high phenolic, flavonoid contents and potential antioxidant activity and could be used as a viable source of natural antioxidants and might be exploited for functional foods and nutriceuticals applications.