Effect of side stream cigarette smoke exposure on antioxidant defense on mice lung tissue and prevention by vitamin-E and vitamin-C (original) (raw)

Objective: Chronic cigarette smoke (CS) induces significant inflammation leading to emphysema due to an imbalance of oxidant/antioxidant mechanism. Methods: The mice were randomly divided into five groups of eight mice’s each. Wistar mice were exposed to cigarette smoke for 16 weeks, 2 cigarettes for 15 min/ twice daily, 6 days/wk. The control animals (C) inhaled clean room air. Biochemical markers in blood and lung tissues were analyzed. Results: Present study revealed that the CS induced oxidative stress enhanced the lipid peroxidation indicated by augmented levels of thiobarbituric acid reactive substances (TBARS) in lung tissue. The increased activity levels of glutathione-S-transferase (GST), decreased activity levels of superoxide-dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) in the lung tissue of CS exposed animals indicate the augmented oxidative stress. The antioxidants vitamins duo E and C were significant decreased in the serum of CS exposed animals. The studies on the Bronchoalveolar lavage (BAL) revealed alveolar macrophage apoptosis in CS animals. Conclusions: The present study revealed that the supplementation of vitamin E and C (50 mg/kg body weight) attenuate the effects of oxidative study in CS exposed animals.

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