Biochemical changes induced by Butachlor, pre-emergent herbicide to sub lethal concentrations in the freshwater fish Labeo rohita (Hamilton) (original) (raw)

The present study was conducted to investigate the effects of acute and chronic toxicity of Butachlor, herbicide on biochemical indices of Indian major carp Labeo rohita. The median lethal concentration (LC50) of Butachlor was found to be 0.85 mgl-1 by using Finney’s Probit Method. Fish were exposed to sublethal doses of 1/10th 96 h LC50 (0.21 mgl-1) and the alterations were evaluated by using various methods for the period of 1day and 10 days respectively. A significant increase in AST, activity was observed in muscle (75.82 % - highest), kidney (0.88% - lowest), ALT activity was noticed in kidney (64.82 % - highest) and in muscle (2.70 % - lowest), the ACP activity level elevated was observed in kidney (28.82 % - highest) and in gill (1.71% - lowest). The acetyl cholinesterase (AChE) enzyme decreased was observed in kidney (52.24 % - highest) and in muscle (1.17 % - lowest) for 1day. Similarly, during 10 days sublethal exposure, AST activity elevated in kidney (86.59 % - highest) and intestine (58.96 % - lowest), ALT activity also increased in kidney (138.66 % - highest) and (42.01 % - lowest) in gill tissues. The ACP percent change was noticed in brain (48.64% - highest) and (26.51% - lowest) in kidney. The AChE activity decreased in kidney (75.17% - highest) and in intestine (48.84 % - lowest), compared to the control group fish (P < 0.05) during the experimentation. Hence, the percent change of biochemical constituent’s has gradually increased or decreased due to of internal organ in all tissues. This might be due to enhanced enzyme turnover under pesticide stress.