Ecological and physiological analyses of Pseudomonad species within a phenol remediation system (original) (raw)
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Phenol is a toxic pollutant found in effluent of numerous industries and its elimination is a foremost challenge. The utilization of bacteria plays a crucial role in phenol bioremediation. For isolation of phenol degrading bacteria, sample was collected from industrial waste and enriched in mineral salt medium (MSM) contained 300 mg/L phenol. The strain was identified based on 16S rRNA gene analysis as Pseudomonas species and the phylogenetic analysis affiliated the strain with Pseudomonas monteilii(AF064458) as the most closely related species. Phenol tolerance of the strain in MSM supplemented with various concentrations of phenol indicates that the strain NCCP-407 can grow best at 750 mg L−1 phenol. The strain showed complete degradation of 750 mg L−1 phenol in 56 hours when supplement as a sole source of carbon and energy with the average degradation rate of 28 mg L−1h−1. The doubling time was recorded approximately as 12.49 h−1. The present study suggests that this strain is efficient in phenol degradation and can be used in treatment of wastewater containing phenol.
Desalination, 2009
Using the phenol enrichment cultivation method, we succeeded in screening a few bacterial strains with a varied ability for utilizing phenol as the sole carbon source from pharmaceutical disposal wastewaters plant. Among these strains, a potent phenol-degrading bacterium, assigned Pseudomonas sp. SA01, was identified and examined as having the greatest potential for degrading phenol. According to biochemical characteristics and 16S rRNA sequence analysis, the isolate was identified as Pseudomonas sp. The isolated strain started to degrade 0.7 g/l of phenol after an initial very short lag phase, and phenol decomposition was then rapidly completed within 30 h. Pseudomonas sp. SA01 was able to degrade phenol in concentrations up to 1 g/l. Higher phenol concentrations (>1 g/l) had a significant inhibitory effect on bacterial growth. The optimum degradation pH value was found to be 6.5. Addition of mannitol and casein as auxiliary carbon and nitrogen sources enhanced the rate of phenol removal to as low as 20 h. Based on the absorption spectra of catechol bioconversion of phenol-grown cells, it was concluded that the SA01 strain metabolizes the phenol via a meta-cleavage pathway.
International Journal of …, 2008
The potential of microorganisms to catabolise and metabolise xenobiotic compounds has been recognised as a potentially effective means of toxic and hazardous wastes disposal. Phenol and its derivatives have long been recognised as some of the most persistent chemicals in petroleum refinery wastewaters, with high toxicity even at low concentrations. Biodegradation of these compounds has been recognised as a potential solution for their disposal owing to its cost effectiveness and simplicity. Two species of pseudomonas, P. aeruginosa and P. fluorescence, were studied for their biodegradation potential on phenol present in a refinery wastewater under a batch fermentation process. Phenol was successfully degraded by both species, and there was high positive correlation between phenol biodegradation and microbial growth. The maximum specific growth rate were obtained for both species from the Haldane model. The study revealed the high potential of these local strains, with P. aeruginosa being more effective, and the possibility of using them in bioremediation of petroleum refinery wastewaters.
2021
This study examines the isolation and characterization of phenol-degrading-bacteria, Five isolates named C2, P4, M4, O3, and S3, from the effluent of ceramic factories (C2), petrochemical (P4), the pulp paper mill (M4), crude oil (O3) and sewage water (S3) have been investigated. These isolates were characterized depending on their morphological, and biochemical characteristics, only one isolate was characterized as a Gram-positive, strictly aerobic, nonmotile, and cocci-shaped bacterium might be Micrococcus sp. (M4), two isolates (Pseudomonas sp.: (P4&S3) were recogniced as a Gram-negative, strictly aerobic, motile and short rod-shaped bacterium and two isolates(Bacillus sp.: (C2& O3) were idenihied as a Gram-positive, strictly aerobic, motile and long rod- shape bacterium. The five bacterial isolates were able to utilize phenol as a sole carbon source. These isolates were checked for growth on a minimal salt medium amended with different concentrations of phenol. The five tolerant...
2021
To identify phenol degrading isolate, a phenotypic fingerprint is produced in a characteristic pattern from discrete test reactions performed using Gen III Micro log, a biolog microtitre plate assay. Six strains were isolated from phenol contaminated effluent, KP-1 to KP-6. Screening was done at different concentrations from 0.2 to 1.4 g/l. Relationship between growth and degradation of the isolate suggested that the strain KP-3 could degrade 92.7% of phenol optimally at 36oC, pH 7.5 and 20 g/l of phenol. This study demonstrated that Pseudomonas strain KP-3 had strong ability to degrade.
Microbial Degradation of Phenol by an Application of Pseudomonas mendocina
Austin Publishing Group
The present investigation was undertaken to isolate and assess the microbial degradation of phenol by bioaugmentation of Pseudomonas mendocina isolated from activated sludge of Common Effluent Treatment Plant (CETP) based at Ankleshwar (Gujarat, India). The strain was isolated and designated as Pseudomonas cepacia ETL 2413 after examined for colony morphology, gram stain characteristics and various biochemical tests. Pseudomonas cepacia ETL 2413 was found to be highly effectual for the removal of phenol which was used as sole carbon and energy source. From an initial concentration of 200mg/L, it degraded to 76.43 ± 1.23 mg/L. In turn the effect of temperature (25 to 500C), pH (5.5 - 10.5) and glucose concentration (0, 0.25 and 0.5%) on the rate of phenol degradation was investigated. Observations revealed that the rate of phenol biodegradation was affected by pH, temperature of incubation and glucose concentration. The optimal conditions for phenol removal were found to be pH of 7.5 (82.63% removal), temperature 300C (78.69% removal) and 0.25% supplemented glucose level (98.28% removal). The significance of the study is the utilization of native bacterial strains isolated from the waste water itself having potential for environmental bioremediation in the activated sludge process of a CETP Plant.
Applied and environmental microbiology, 1998
DNA was isolated from phenol-digesting activated sludge, and partial fragments of the 16S ribosomal DNA (rDNA) and the gene encoding the largest subunit of multicomponent phenol hydroxylase (LmPH) were amplified by PCR. An analysis of the amplified fragments by temperature gradient gel electrophoresis (TGGE) demonstrated that two major 16S rDNA bands (bands R2 and R3) and two major LmPH gene bands (bands P2 and P3) appeared after the activated sludge became acclimated to phenol. The nucleotide sequences of these major bands were determined. In parallel, bacteria were isolated from the activated sludge by direct plating or by plating after enrichment either in batch cultures or in a chemostat culture. The bacteria isolated were classified into 27 distinct groups by a repetitive extragenic palindromic sequence PCR analysis. The partial nucleotide sequences of 16S rDNAs and LmPH genes of members of these 27 groups were then determined. A comparison of these nucleotide sequences with th...
Phenol is one of the organic pollutants in various industrial wastewaters especially petrochemical and oil refining. Biological treatment is one of the considerable choices for removing of phenol present in these wastewaters. Identification of effective microbial species is considered as one of the important priorities for production of the biomass in order to achieve desirable kinetic of biological reactions. Basic purpose of this research is identification of phenol-degrading Pseudomonas Putida in activated sludge by polymerase chain reaction (PCR) that has high speed and specificity. In this research, 10 various colonies of phenol-degrading bacteria were isolated from municipal activated sludge and the rate of phenol removal and growth rate of these bacteria were assessed in different concentrations of phenol (200 – 900 mg/L). Confirmation of the largest subunit of multicomponent phenol hydroxylase (LmPH) gene and gene coding the N fragment in Pseudomonas Putida-derived methyl ph...
2014
A strain of Pseudomonas aeuriginosa MTCC 4997 isolated newly from effluents collected from petrochemical industries. The isolated strain was optimized for various operational and environmental conditions in batch culture. This strain was utilizes phenol as a sole source of carbon and energy, capable of degrading phenol up to 1400 mg l -1 concentration within 144 h. Complete degradation was observed at wide temperature from 15 0 C to 45 0 C with an optimum of 37 0 C and pH 6.0 to 10.5 with an optimum of 7.0 to 7.5. Metals such as Fe, Cd, Cu, Pb, Zn, Mn and Ba at lower concentrations were found stimulated and enhanced the rate of phenol degradation. Therefore, the phenol degradability of the strain can be maintained and used at large scale treatment.