Microorganism Screening for Limonene Bioconversion and Correlation With RAPD Markers (original) (raw)

Biotransformation of R-(+)- and S-(‒)-limonene by Fusarium oxysporum

2011

In the past few years much work has been done on th e biotransformation of limonene, an inexpensive hydrocarbon monoterpene, which is one of the most w idely distributed terpene in nature. Recently, the strain F. oxysporum 152b has been selected based on its high productio n of extracellular alkaline lipase and other publications have also described the biotransformat ion of R-(+)-limonene into R-(+)-α-terpineol catalysed by the same strain. The main characteristics of this c onversion were described in recent papers but no information deals with the bioconversion of its iso mer, S-(‒)-limonene. Thus, this paper focused on the biotransformation of S-(‒)-limonene by F. oxysporum 152b, partially characterized and compared with th e characteristics involved in α-terpineol production from R-(+)-limonene. In former studies, it was proven tha t the enzyme responsible for the production of α-terpineol by the biotransformation of R-(+)-limonene was enantioselective and enantiospecific, has ...

Biosynthesis of C10H16 (Limonene) by Mevalonate pathways and its pharmacological applications

International Journal of Biology and Biotechnology, 2019

Limonene is a transparent, colourless compound (chemical) has D and L isomer; which is found in oil of citrus fruits peels. It is a monoterpene (monocyclic) hydrocarbon and used in the production of several commodity chemicals, biodiesel products, medicinal compounds and pharmaceutical and it is also an important precursor of many flavouring. Limonene is having low toxicity and can be used for several purposes. Overproduction of Limonene can be achieved by genetic engineering of Mevalonate pathway and 2-C-methyl-D-erythritol-4-phosphate pathway (MEP and MVA) in microorganisms by introducing limonene synthase. Limonene has several applications in industrial products and also can be used in clinical or medical field. Moreover the metabolic engineering of the pathways is potential production platform for any valuable limonene derivative. Limonene is flammable, function as biofuel. Limonene is naturally occurring in plants but genetically engineered microbes could produce sufficient qua...