Effect of Probiotic, Carbohydrase Enzyme and Their Combination on the Performance, Histomorphology and Gut Microbiota in Broilers Fed Wheat-based Diets (original) (raw)

Abstract

The effect of supplementation of probiotic and/or carbohydrase on performance and gut health in broiler chickens was studied. Diet 1 was the basal diet with no supplement while diets 2, 3, 4 and 5 were supplemented with antibiotic, probiotic, carbohydrase and combination of probiotic and carbohydrase, respectively in a randomized complete block design. The experiment was carried out at the Poultry Unit of the Teaching and Research Farm, University of Ibadan, Nigeria between March and April, 2013. 240 one-day-old broiler chickens were allotted to 5 diets with 6 replicates of 8 birds each in a 35-day experiment. On day 35, birds were sacrificed and digesta samples from the duodenum, ileum, caecum and colon were collected for microbial load count. Digesta viscosity and pH from the ileum were also determined. Sections of the ileum were obtained for morphological measurements. Result showed that diet had no effect on the feed intake, dry matter intake and feed conversion ratio of birds at starter (d 0-21), grower phases (d 22-35) and overall period (d 0-35). Body weight gain was significantly (P = .05) improved at d 0-21 and 22-35 with the addition of probiotic and/or carbohydrase but not at d 0-35. Diet had no effect on the microbiota in the various segments of the GIT of birds. Coliform counts of birds fed diet supplemented with carbohydrase were significantly (P = .05) higher (5.64 x 106 cfu/ml) than the values in other diets. The crypt depth, villus height, villus width and villus height:crypt depth ratio were not affected by diet. Highest digesta viscosity (1072mPa) was recorded in birds on the negative control while the least digesta viscosity (529mPa) was observed in birds on diet supplemented with carbohydrase enzyme. It is concluded that probiotic and/or carbohydrase are viable feed supplements in wheat-based diets for broilers.

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References (45)

  1. Mathur S, Singh R. Antibiotic resistance in food lactic acid bacteria: A review. International Journal of Food Microbiology. 2005;105:281-295.
  2. EU (European Commission). Regulation 1831 of the European Parliament and of the Council of 22 September 2003 on additives for use in animal nutrition. Official Journal of European Union L. 2003; 268/29.
  3. EU (European Communities). Commission of the European Communities, Commission Regulation No. 1831/2003. Official Journal of European Union. 2006; 268:29-43.
  4. Williams J, Mallet S, Leconte M, Lessire M, Gabriel I. Effects of fructo-oligosaccharides or whole wheat on the performance and digestive tract of broiler chickens. British Poultry Science. 2008;49(3):329-339.
  5. Agboola AF, Aroniyo I, Suberu SA, Adeyemi WT. Dietary supplementation of probiotics and synbiotics on intestinal microbial populations and gut morphology of turkey poults. African Journal of Livestock Extension. 2014a;14:13-20.
  6. Kim JJ, Khan WI. Goblet cells and mucins: Role in innate defense in enteric infections. Pathogens. 2013;2(1):55-70.
  7. Kizerwetter-Swida M, Binek M. Protective effect of potentially probiotic Lactobacillus strain on infection with pathogenic bacteria in chickens. Polish Journal of Veterinary Science. 2009;12:15-20.
  8. Markovic R, Šefer D, Krstic M, Petrujkic B. Effect of different growth promoters on broiler performance and gut morphology. Archives of Medical Veterinary. 2009;41: 163-169.
  9. Agboola AF, Omidiwura BRO, Odu O, Adeyemi WT, Suberu SA, Aroniyo I, et al. Influence of dietary supplementation of probiotics and symbiotics on growth performance, nutrient digestibility and organ weights in turkey poults. Ibadan Journal of Agricultural Research. 2014b; 10(10) (In Press).
  10. Midilli M, Alp M, Kocabağli N, Muğlalı ÖH, Turan N, Yılmaz H, et al. Effects of dietary probiotic and prebiotic supplementation on growth performance and serum IgG concentration of broilers. South African Journal of Animal Science. 2008;38:21-27.
  11. Irani M, Gharahveysi S, Rahmatian R, Zamani M, Pour VR. The effect of butyric acid glycerides on serum lipids and carcass analysis of broiler chickens. African Journal of Biotechnology. 2011; 10(53):11087-11092. ISSN 1684-5315.
  12. Yusrizal N, Chen TC. Effect of adding chicory fructons in feed on broiler growth performance, serum cholesterol and intestinal length. International Journal of Poultry Science. 2003;2:214-219.
  13. Gaggia F, Mattarelli P, Biavati B. Probiotics and prebiotics in animal feeding for safe food production. International Journal of Food Microbiology. 2010;141:15-28.
  14. Yalcinkaya I, Gungor T, Basalan M, Erdem E. Mannan oligosaccharides (MOS) from Saccharomyces cerevisiae in broilers: Effects on performance and blood biochemistry. Turkish Journal of Veterinary Animal Science. 2008;32:43-48.
  15. Choct M. Managing gut health through nutrition. British Poultry Science. 2009;50: 9-15.
  16. Gutierrez A, Verstegen MW, Den Hartog LA, Perez P, Villamide MJ. Effect of wheat cultivar and enzyme addition to broiler chicken diets on nutrient digestibility, performance and apparent metabolizable energy content. Poultry Science. 2008;87: 759-767.
  17. Jaroni MA, Scheideler SE, Beck MM, Wyatt C. The effect of dietary wheat middling and enzyme supplementation 11: Apparent nutrient digestibility, digestive tract size, gut viscosity and gut morphology in two strains of leghorn hens. Poultry Science. 1999;78:1664-1674.
  18. Broz J, Ward NE. Effect of enzyme supplementation of feed enzymes in combating metabolic challenges and disorders. Journal Applied Poultry Research. 2007;16:150-159.
  19. Guilherme F, Goulart CC, Figueiredo DF, Oliveira CFS, Silva JHV. Economic and environmental impact of using exogenous enzymes on poultry feeding. International Journal of Poultry Science. 2008;7:311- 314.
  20. Agboola AF, Fagbemi MA, Oluwadamilare EI, Majolagbe OH. Effect of Roxazyme G2G supplementation on performance and carcass characteristics of broilers fed wheat-based diets. International Journal of
  21. Konca Y, Figen K, Selim M. Effects of mannan-oligosaccharides and live Yeast in diets on the carcass, cut yields, meat composition and colour of finishing turkeys. Asian Australasian Journal of Animal Science. 2009;22:550-556.
  22. Iyayi EA, Yahaya BA. Performance of broilers maintained on diets supplemented with commercial polysaccharidase enzyme in the Tropics. Tropical Animal Production Investigation. 1999;122-130.
  23. Bedford MR. Antibiotic growth promoters from poultry diets: implications and strategies to minimize subsequent problems. World Poultry Science Journal. 2000;56:347-365.
  24. Higgins SE, Higgins JP, Wolfenden, AD, Henderson SN, Torres-Rodriguez A, Tellez G, et al. Evaluation of a Lactobacillus- based Probiotic Culture for the Reduction of Salmonella enteritidis in Neonatal Broiler Chicks. Poultry Science. 2008;87:27-31.
  25. Vila B, Fontgibell A, Badiola I, Esteve- Garcia E, Jiménez G, Castillo M, et al. Reduction of Salmonella enterica var. enteritidis colonization and invasion by Bacillus cereus var. Toyoi inclusion in poultry feeds. Poultry Science. 2009;88: 975-979.
  26. Watkins BA, Kratzer FH. Effect of oral dosing of Lactobacillus strains on gut colonization and liver biotin in broiler chicks. Poultry Science. 1983;62:2088- 2094.
  27. Jin LZ, Ho YW, Abdullah N, Jalaludin S. Growth performance, intestinal microbial populations and serum cholesterol of broilers fed diets containing Lactobacillus cultures. Poultry Science. 1998;77:1259- 1265.
  28. Rahmani HR, Speer W. Natural additives influence the performance and humoral immunity of broilers. International Journal of Poultry Science. 2005;4(9):713-717.
  29. Howard MD, Kerley MS, Gordon DT. Effect of dietary addition of fructooligosaccharide on colonic microflora populations and epithelial cell proliferation in neonatal pigs. Journal of Animal Science. 1993;1:71-77.
  30. Choi KH, Namkung H, Paik IK. Effects of dietary fructooligosaccharides on suppression of intestinal colonization of Salmonella typhimurium in broiler chickens. Korean Journal of Animal Science. 1994;36:71-284.
  31. WGO (World Gastroenterology Organization). Probiotics and Prebiotics. World Gastroenterology Organization Practice Guidelines; 2008.
  32. Edwards CA, Johnson II, Read NW. Do viscous polysaccharides slow absorption by inhibiting diffusion or convection? European Journal of Clinical Nutrition. 1988;42:307-312.
  33. Ikegami S, Tsuchihashi F, Harada H, Tsuchihashi N, Nishide E, Innami S. Effect of various indigestible polysaceharides on pancreatic -biliary secretion and digestive organs in rats. Journal of Nutrition. 1990; 120:353-360.
  34. Smith CHM, Annison G. Non-starch polysaccharides in broiler nutrition: Towards a physiological valid approach to their determination. World's Poultry Science Journal. 1996;52:203-221.
  35. Bedford MR. The role of carbohydrases in feedstuff digestion, in: Editors Mcnab, J. & Boorman, K. N. Poultry feedstuffs: Supply, composition, and nutritive value, (CAB international, Edinburgh, UK). 2001;319- 336.
  36. Bedford MR, Classen HL. An in-vitro assay for prediction of broiler intestinal viscosity and growth when fed rye based diets in the presence of exogenous enzymes. Poultry Science. 1993;72:137-143.
  37. Ritz CW, Hulet RM, Self BB, Denbow DM. Growth and intestinal morphology of male turkey as influenced by dietary suplementation of amylase and xylanase. Poultry Science. 1995;74:1329-1334.
  38. Mathlouthi N, Lallès JP, Lepercq P, Juste C, Larbier M. Xylanase and glucanase supplementation improve conjugated bile acid fraction in intestinal contents and increase villus size of small intestine wall in broiler chickens fed a rye-based diet. Journal of Animal Science. 2002;80:2773- 2779.
  39. Viveros A, Brenes A, Pizarro M, Gastano M. Effect of enzyme supplementation of a diet based on barley and autoclave treatment on apparent digestibility, growth performance and gut morphology of broilers. Animal Feed Science Technology. 1994;48:237-251.
  40. Caspary WF. Physiology and pathophysiology of intestinal absorption. American Journal of Clinical Nutrition. 1992;55(1):299-308.
  41. Yason CV, Summers BA, Schat KA. Pathogenesis of rotavirus infection in various age groups of chickens and turkeys: Pathology. American Journal of Veterinary Research. 1987;6:927-93.
  42. Chiou PWS, Lu TW, Hsu JC, Yu B. Effect of different sources of fiber on the intestinal morphology of domestic geese. Asian- Australasian Journal of Animal Science. 1996;4:539-550.
  43. Oliveira MC, Rodrigues EA, Marques RH. Gravena RA , Guandolini GC, Moraes VMB. Performance and morphology of intestinal mucosa of broilers fed mannan- oligosaccharides and enzymes. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte. 2008;60(2).
  44. Montagne L, Pluske JR, Hampson DJ. A review of interactions between dietary fibre and the intestinal mucosa and their consequences on digestive health in young non ruminant animals. Animal Feed Science Technology. 2003;103:95-117.
  45. _________________________________________________________________________________