Fungal dysbiosis in mucosa-associated microbiota of Crohn's disease patients (original) (raw)

Journal of Crohn's & colitis, 2015

Abstract

Gut microbiota is involved in many physiological functions and its imbalance is associated with several diseases, particularly with inflammatory bowel diseases. Mucosa-associated microbiota could have a key role in induction of host immunity and in inflammatory process. Although the role of fungi has been suggested in inflammatory disease pathogenesis, the fungal microbiota has not yet been deeply explored. Here we analyzed the bacterial and fungal composition of the mucosa associated microbiota of Crohn's disease patients and healthy subjects. Our prospective, observational study evaluated bacterial and fungal composition of mucosa-associated microbiota of 23 Crohn's disease patients (16 in flare, 7 in remission) and 10 healthy subjects using 16S (MiSeq) and ITS2 (pyrosequencing) sequencing, respectively. Global fungal load was assessed by real time quantitative PCR. Bacterial microbiota in Crohn's disease patients was characterized by a restriction in biodiversity with...

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