Molecular Cytogenetics Manual (original) (raw)

Advances in the field of cytogenetics, largely, have been technology driven. We have witnessed multiple waves of progression starting with accidental discovery of hypotonic saline to the current era of molecular cytogenetics. The new wave is now on chromosome analysis from microscope to automated microarray (molecular karyotyping) for identification of chromosomal abnormality beyond cytogenetic resolution. The days are not far when microarray based molecular karyotyping would become the primary choice to direct analysis of all chromosomes/genes/portion of genes in one action, without subjective nature and difficult cell culture, the real replacement of conventional cytogenetics for most purpose and without restriction to cytogenetic experts. The proposed workshop has been planned to provide an exposure to molecular cytogenetics: Reproductive genetics by fluorescent in situ hybridization (FISH), Primed In Situ Labeling (PRINS) and Polymerase Chain Reaction (PCR). Besides few lectures...