A new HLA-CW14 allele, Cw140204, identified by allele-specific DNA amplification and sequencing (original) (raw)
Related papers
Tissue Antigens, 2002
Abstract: This paper describes 10 novel HLA-A alleles that have been characterized by DNA sequencing. Seven alleles, A*0308, A*2616, A*3009, A*3206, A*3403, A*3602 and A*6604 carry motifs observed in other HLA-A alleles, suggesting that gene conversion has created this diversity. The remaining three alleles, A*01012, A*0306 and A*2617, contain polymorphisms not previously found in any “classical” class I allele. All alleles were identified due to unexpected probe hybridization patterns during routine SSOP typing. Exons 2 and 3 of each allele were subsequently characterized by DNA sequencing.
Tissue Antigens, 2008
We describe two novel human leukocyte antigen (HLA) alleles, HLA-A*02010103 and HLA-B*4455, that were discovered in two unrelated Caucasian individuals. In addition, we report the full-length genomic sequence of HLA-A*290201. Compared with HLA-A*02010101, HLA-A*02010103 has three nucleotide (nt) changes within intron 1, which is altered to a sequence typical of the HLA-A*23/A*24 allele group. In HLA-B*4455, an nt exchange occurred in codon 9 of HLA-B*44020101, resulting in a change of the amino acid coding from tyrosine to histidine. We sequenced HLA-A*290201 from nt −108 to nt 2922, encompassing all exons and introns as well as parts of the 5′ and 3′ untranslated regions. Previously, the full-length genomic sequence was known only for HLA-A*29010101, which is found at a lower frequency in Caucasians than HLA-A*290201.
HLA Class I Nucleotide Sequences, 1991
Human Immunology, 1991
The H~I class I sequences included in this compilation are taken from publications listed in the accompanying paper, Nomenclature for factors of the HLA system, 1990 and Nomenclature for factors of the HLA system, 1989). Where discrepancies have arisen between reported sequences the original authors have been contacted where possible, and necessary amendments to published sequences have been incorporated into this alignment. Future sequencing may identify errors in this list and we would welcome any evidence that helps to maintain the accuracy of this compilation. In the sequence alignments identity between residues is indicated by a hyphen (-). Unavailable sequence is indicated by a period (.). Gaps in the sequence are inserted to maintain the alignment between different alleles showing variation in amino acid number.
Human Immunology, 2006
We report here four novel human leukocyte antigen (HLA)-A alleles identified among an East African population during sequence-based HLA-A typing. The novel alleles were confirmed by sequencing two separate polymerase chain reaction products and by molecular cloning and sequencing multiple clones. The new allele A*9202 is identical to A*0202 at exon 2 and exon 3 except for a single nucleotide difference at codon 43 (CGG-->CAG), resulting in a coding change from Arginine to Glutamine. The second new allele has a synonymous change at codon 139 (GCA-->GCG), that differentiates it from A*680101. The new allele has been named by the World Health Organization nomenclature committee as A*680105. The novel allele A*2630 is identical to A*2603 at exon 2 and exon 3 except for a nonsynonymous change at codon 90 (GAC-->GCC), changed from Aspartic acid to Alanine. The fourth new allele is identical to A*290201 except for a single nucleotide difference at codon 138 (ATG-->GTG), resulting in a coding change from Methionine to Valine. The new allele has been named by the World Health Organization nomenclature committee as A*2915. Identification of these novel HLA-A alleles reflects the genetic diversity of this East African population.
Exon/intron organization and complete nucleotide sequence of an HLA gene
Proceedings of the National Academy of Sciences, 1982
We have determined the complete nucleotide sequence of a human class II histocompatibility antigen DCl gene. The gene spans more than 7 kilobases and contains five exons corresponding to the different domains of the DC,8 polypeptide. The exon-intron organization is thus analogous to that of class II antigen a-chain genes, class I antigen heavy chain genes, and the constant parts of immunoglobulin genes, emphasizing further the evolutionary relationship among these molecules. The mature polypeptide deduced from the DC.8 gene shows 93% and 88% homology, respectively, to sequences derived from two DC.8 cDNA clones of other haplotypes. The allelic polymorphism of DC.8 chains resides predominantly in the first extracellular domain, whereas the rest of the polypeptide is virtually constant. The exons of the DCfi gene display high homology to the corresponding exons of a murine I-Ar gene. Also, the introns show significant homology. The DCI3 chains lack eight amino acids in the cytoplasmic tail, as compared to DR and I-A .8 chains. This is probably due to a nonfunctional splice junction of DC13 genes, causing a separate cytoplasmic exon to be nonexpressed.
HLA-A*3306, a novel variant in the Indian population
Tissue Antigens, 2002
We describe a new HLA-A allele, A*3306, which was identified by sequencing based typing (SBT) in an individual of Indian origin. A*3306 is similar to A*3303, with a difference at position 228 (A to G). This difference leads to an amino-acid change at codon 52 from Ile (ATA) to Met (ATG). Until now this position has been considered conserved.