Structure to Function: High-Throughput Multi-Stage Tandem Mass Spectrometry Platform for the Characterization of Lipid A Extracts From Gram-Negative Bacteria (original) (raw)

Abstract

Lipopolysaccharide (LPS) constitutes the majority of the outer layer of the outside membrane of Gram-negative bacteria and represents one of the microbial molecular signals responsible for activation of the mammalian innate immune system. Recognition of LPS, in particular its bioactive component lipid A, has provided mammalian immune systems with a key strategy for defense against many Gram-negative pathogens. Previous studies have shown that alteration of lipid A structure greatly affects bacterial virulence serving as a survival strategy. Structural diversity in lipid A is often exhibited in the fatty-acid position and composition, phosphorylation and terminal monosaccharide modifications. A high-throughput multi-stage tandem mass spectrometry platform has been developed with the emphasis on characterizing diagnostic product ions from the dissociation of gas-phase lipid A anions. These diagnostic product ions provide routine and comprehensive structure determination for a variety ...

Jace Jones hasn't uploaded this paper.

Let Jace know you want this paper to be uploaded.

Ask for this paper to be uploaded.