Association between the occurrence of matrix metalloproteinases 2 and 9 in parotid saliva with the degree of parotid gland damage in juvenile recurrent parotitis (original) (raw)
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Molecular Alterations of Parotid Saliva In Infantile Chronic Recurrent Parotitis
Pediatric …, 2007
nfantile chronic recurrent parotitis (ICRP) is an insidious disease whose etiopathogenesis remains an enigma. Alterations in the physical appearance of parotid saliva from ICRP patients have been frequently reported. However, sialochemical studies in regard to ICRP are very rare. The aim of this study was to determine whether saliva of ICRP patients presents major physicochemical and biochemical alterations compared with saliva from paired healthy controls. Parotid, whole, and submandibular/sublingual saliva was collected at an asymptomatic stage from 33 ICRP patients (5-16 y old, both sexes) and from 33 sex- and age-matched healthy controls. Saliva was analyzed for protein concentration, mode of protein diffusion on cellulose membranes, unidimensional sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis protein profiles and zymographic profiles of metalloproteinase 2 (MMP-2) and metalloproteinase 9 (MMP-9). Parotid saliva of ICRP patients showed an increased protein concentration, altered mode of protein diffusion, a higher frequency of polypeptide bands of 43, 37, 33, 29, 26, 16, and 10 kD, higher asymmetry in the polypeptide profiles of both contralateral parotid saliva, and an increase in the frequency of MMP-2 and MMP-9. Parotid saliva of patients with ICRP is molecularly altered with respect to normal saliva. Some of the molecular differences could be related to the etiopathogenesis of the disease. Abbreviations: ICRP, infantile chronic recurrent parotitis MMP-2, metalloproteinase 2 MMP-9, metalloproteinase 9
Dental and Medical Problems
Background. Dental caries is initiated through mineral dissolution by bacterial acids and collagen de gradation by endogenous proteolytic enzymes, mainly collagenolytic matrix metalloproteinases (MMPs). Objectives. The present research aimed to evaluate the relationship between severe early childhood caries (SECC) and salivary MMP8 and MMP20 concentrations. Material and methods. Fifty children aged 36-60 months were assigned to either the cariesfree (control) group or the SECC group. Standard clinical examinations were performed, and approx. 1 mL of expectorated unstimulated whole saliva was collected from all participants. In the SECC group, the sampling was repeated 3 months after restorative treatment. All samples were analyzed for the salivary concentrations of MMP8 and MMP20, using the enzymelinked immunosorbent assay (ELISA). Statisti cal analysis employed the t test, the Mann-Whitney U test, the χ 2 test, Fisher's exact test, and the paired samples t test. The level of significance was set at 0.05. Results. At baseline, the subjects in the SECC group presented with significantly elevated levels of MMP8 as compared to the control group. However, the salivary concentration of MMP20 did not exhibit a signifi cant difference between the 2 groups. A significant reduction occurred in the levels of MMP8 and MMP20 3 months after restorative treatment in the SECC group. Conclusions. The salivary levels of MMP8 and MMP20 were significantly affected by dental restorative treatment in children. Furthermore, MMP8 was observed to be a better indicator of the dental caries status than MMP20.
InTech eBooks, 2012
Several causing factors have been invoked, namely, congenital duct malformations, genetic factors, allergies, local manifestations of autoimmune diseases, immunodeficiencies, maxillary disfunction due to tooth misalignment, etc. (Baurmash, 2004; Chitre & Premchandra, 1997; Bernkopf et al., 2008). None of these factors has been unequivocally demonstrated as a causing agent and to date the etiopathogenesis of the disease remains an enigma. In attempts to elucidate the origin of this clinical condition, a number of microbiological analyses of parotid secretion have been carried out. By direct analysis of parotid saliva from ICRP patients, parotitis virus and several respiratory virus have been discarded as eventual causing agents (Landaeta et al., 2003). Bacteriological analyses by Concheiro et al. (2000) have shown the presence of a mixed flora of Gram-positive cocci. Giglo et al. (1997) have shown that 80% of patients display Streptococcus pneumoniae, Haemophilus influenzae, Streptococcus viridans y/o Moraxella catarrhalis. Despite the relevance of these insights into the disease, several characteristics of the disease challenge its infectious origin. Among them we can remark that the glandular process is mainly of inflammatory nature, that the general health condition of the patient is just moderately affected, the unilateralness of the condition among patients carrying the above-mentioned flora and, finally, the occurrence of a number of cases in which cultures for bacterial microorganisms give negative results. In the specialized literature expressions like "At the present there is a tendency to consider a multifactorial origin of the disease" have become quite usual. According to this undemonstrated view, sialectasis would be the ICRP-triggering factor. Thus, due to partial malformations of the duct system, iterated ascending infections would result in symptomatic episodes and reinforced the duct alterations (
International Journal of Cancer Management
Background: Head and neck squamous cell carcinoma (HNSCC) is the sixth common cancer worldwide. A hallmark of cancer progression is degradation of the extracellular matrix by matrix metalloproteinases (MMPs) that allows cancer cells to invade the surrounding tissue. Objectives: The purpose of this study was to evaluate the levels of MMP-2 and 9 in serum and salivary of HNSCC patients and compare it with a healthy group. Methods: Twenty patients with newly diagnosed HNSCC, who had not received any treatment, referred to Omid and Ghaem hospitals, Mashhad, Iran, and twenty healthy controls were voluntarily included in this study. Salivary and blood samples were collected from both groups and the concentration of MMP-2 and MMP-9 were measured by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed by SPSS using Student t-test or Mann-Whitney with significance level of ≤ 0.05. Results: In cancer patients, the serum level of MMP-9 was significantly higher than that of in the healthy group (P < 0.001). However, salivary MMP-9 was higher in cancer patients, this difference was not significant (P = 0.736). There was no significant difference between the study groups for the levels of serum (P = 0.283) and salivary MMP-2 (P = 0.764). There was a correlation between salivary and serum levels of both markers in cancer patients (P = 0.046 and P = 0.011 for MMP-2 and MMP-9, respectively), on the contrary, there was not a correlation between them in the healthy controls (P = 0.628 and P = 0.064, for MMP-2 and MMP-9, respectively). A direct correlation between the salivary level of MMP-9 and tumor grade was also detected (P = 0.045). Conclusions: The salivary analysis indicates that an altered composition for MMPs in HNSCC, suggesting a potential diagnostic tool for oral cancer. The serum level of MMP-9 appears to be a reliable marker for early diagnosis in HNSCC patients.
Report of a rare case of juvenile recurrent parotitis and review of literature
European Archives of Paediatric Dentistry, 2009
BACKGROUND: Juvenile recurrent parotitis (JRP) is a rare salivary gland disease of obscure aetiology that affects children. It is characterized by multiple episodes of unilateral or bilateral parotid inflammation over a period of years. CASE REPORT: A 14 year old boy presented with multiple episodes of recurrent bilateral swellings of the parotid glands since 1 year of age with no relevant past medical and dental history, TREATMENT: Included prescription of antibiotic Dicloxacillin 500 mg tid for 7 days and analgesics as a combination of Diclofenac 50 mg and Paracetamol 500 mg tid for 10 days, to resolve acute infection followed by sialography using Iopromide (ultravist-300) twice at an interval of 6 months for glandular lavage which helps to clear the mucous plugs that form during the acute phase. FOLLOW-UP: It was satisfactory as there has been no recurrence of parotitis during 18 months.
INTERNATIONAL JOURNAL OF ADVANCE RESEARCH, IDEAS AND INNOVATIONS IN TECHNOLOGY
It is a rare condition and characterized by multiple episodes of parotid swelling and/or pain associated with fever or malaise over a period of years. Juvenile Recurrent Parotitis (JRP) is defined as recurrent inflammatory parotitis in children of unknown etiology. Salivary gland diseases in children are rare, apart from viral-induced diseases. Clinical diagnostics and especially the surgical treatment are influenced by stringent indications and a less invasive strategy. Due to the rarity of tumors of the salivary glands in children, it is recommended to treat them in a specialized center with greater surgical experience. In most cases, the symptoms resolve spontaneously after puberty but all children should be screened to exclude Sjogren's syndrome, lymphoma and immunodeficiency including human immunodeficiency virus. Parotid abscess results as a complication of primary parotitis and are very rare in paediatrics population, we present a 6-year-old girl who had 15 episodes of recurrent parotid swelling over past 3 months.
The clinical picture of juvenile parotitis in a prospective setup
Acta Paediatrica, 2012
Aim: To characterize the features of juvenile parotitis in a prospective setup and epidemiology.Methods: All children with parotitis admitted to Helsinki University Central Hospital 2005–2010 were recruited. Clinical characteristics, given treatment, outcome, blood leukocyte count, C‐reactive protein, serum amylase and trypsinogen, SPINK‐1 genotype and mumps antibodies were recorded. To map the epidemiology, a questionnaire was sent to 1000 randomly selected 13‐year‐old children.Results: The prospective study included 41 children (aged ≤ 17) with acute parotitis, all in good general condition. Serum amylase, but not trypsinogen, was elevated in majority of the cases (79%) and C‐reactive protein in 68%. Eleven (27%) children had an elevated blood leukocyte count. None had acute mumps. Most children recovered well, 51% being treated symptomatically only. Seven children were treated on ward. Seventeen (46%) children had recurrent symptoms. One child (2.4%) had SPINK P55S mutation. A...
Major salivary gland diseases. Multicentre study
Acta otorhinolaryngologica Italica : organo ufficiale della Società italiana di otorinolaringologia e chirurgia cervico-facciale, 2005
This multicentre study involved 28 Italian ORL Centres responding to a questionnaire sent by us which allowed recruitment of a high large number of cases of parotid neoplasms observed over a 10-year period. Statistical data obtained partly confirmed previous findings. Benign tumours account for 80% of case histories with a relationship 1:4 M/F, the most frequent being pleomorphic adenoma (57.3% of cases), followed by Warthin's tumour (32.4%), this rating not having been confirmed in case histories (8-10%) in the literature. Malignant tumours instead were fewer in number compared to the literature (14% vs 25-30%); the most frequent being mucoepidermoid carcinoma (18.2%) of which 44% G1, 33% G2 and 23% G3. Adenoid-cystic carcinoma was observed in 15.3% and < or = 10% for all the other most frequent histological malignant neoplasms. Diagnostic work-up included echotomography and fine-needle aspiration biopsy, less used imaging techniques were computed tomography, magnetic resona...
Open Journal of Stomatology, 2020
Currently, diagnosis of chronic periodontitis is based on clinical methods which are cumbersome and have been shown to have inherent errors and drawbacks including inability to detect active disease. There is therefore an urgent need for a timely, cost-effective and less cumbersome method. Salivary matrix metalloproteinase-8 has been shown in some studies to be a promising bio-marker of chronic periodontitis. This was a case-control study conducted at the University College Hospital, Ibadan. It had 40 cases and 40 controls designed to assess the diagnostic ability of salivary MMP-8 in periodontal disease. Unstimulated whole saliva was assessed using the Quantikine human total MMP-8 ELISA kit from R&D ® systems Europe.