Q-Absorbance Ratio Spectrophotometric Method for the Simultaneous Estimation of Betamethasone Sodium Phosphate and Ofloxacin in their Combined Dosage form (original) (raw)
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A Simple, rapid, and sensitive spectrophotometric method was developed for the determination of a fluoroquinolone antibiotic Ofloxacin (OFL), in pure form and pharmaceutical formulations. This method is based on the formation of ion-pair complex between the basic drug (OFL), and acid dye; bromocresol green (BCG). The formed complex was measured at 430 nm by using chloroform as solvent. The analytical parameters and their effects are investigated. Beer's law was obeyed in the range of 0.434 – 11.564 µg/mL, with correlation coefficient R 2 = 0.9998. The average recovery of Ofloxacin was between 98.61 and 101.61%. The limit of detection was 5.78 ng/mL and limit of quantification was 17.52 ng/mL. The proposed method has been successfully applied to the analysis of the studied drug in pure form and pharmaceutical formulations.
Spectrophotometric determination of ofloxacin in pharmaceuticals by redox reaction
Journal of Applied Spectroscopy, 2011
Two simple spectrophotometric methods have been developed to analyze ofloxacin (OFX) in pharmaceuticals. The methods are based on the oxidation of OFX by a measured excess of cerium(IV) sulfate in H2SO4 medium. This was followed by the determination of the unreacted oxidant by reacting it with either p-toluidine ( p-TD) and measuring the absorbance at 525 nm (method A) or o-dianisidine ( o-DA) and measuring the absorbance at 470 nm (method B). In both methods, the amount of cerium(IV) sulfate reacted corresponds to the amount of OFX. Calibration graphs were linear over the ranges of 0-120 and 0-4 g/ml OFX for methods A and B, respectively. The calculated molar absorptivity (2.34ṡ103 and 5.99ṡ104), Sandell sensitivity, and limit of quantification for the methods are reported. The intra-day precision (%RSD) and accuracy (%RE) were < 8.0 and ≤ 4.0%, respectively, and the inter-day RSD and RE values were within 5 and 4.0%, respectively. The applicability of the methods was demonstrated by determining OFX in tablets with an accuracy (%RE) of < 3% and precision (%RSD) of ≤2.65%. The accuracy of the methods was further ascertained by recovery experiments via a standard-addition procedure.
Brazilian Journal of Pharmaceutical Sciences, 2011
One titrimetric and two spectrophotometric methods have been described for the determination of ofloxacin (OFX) in bulk drug and in tablets, employing N-Bromosuccinimide as an analytical reagent. The proposed methods involve the addition of a known excess of NBS to OFX in acid medium, followed by determination of unreacted NBS. In titrimetry, the unreacted NBS is determined iodometrically, and in spectrophotometry, unreacted NBS is determined by reacting with a fixed amount of either indigo carmine (Method A) or metanil yellow (Method B). In all the methods, the amount of NBS reacted corresponds to the amount of OFX. Titrimetry allows the determination of 1-8 mg of OFX and the calculations are based on a 1:5 (OFX:NBS) reaction stoichiometry. In spectrophotometry, Beer's law is obeyed in the concentration ranges 0.5-5.0 µg/mL for method A and 0.3-3.0 µg/mL for method B. The molar absorptivities are calculated to be 5.53x10(4) and 9.24x10(4) L/mol/cm for method A and method B, res...
2015
Simple, fast and reliable spectrophotometric methods were developed for determination of Ofloxacin in bulk and pharmaceutical dosage forms. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 295-301nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ofloxacin using 2-10μg/ml (r²=0.9947) for second order Derivative Area under Curve spectrophotometric method. The proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ofloxacin in pharmaceutical formulations.