Actinomycin binding of normal and phytohaemagglutinin stimulated lymphocytes (original) (raw)

Abstract

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This study investigates the binding of tritium-labelled actinomycin D (3H-AMD) to deoxyribonucleoprotein complexes in phytohaemagglutinin (PHA) stimulated lymphocytes. The findings demonstrate that PHA stimulation significantly increases both RNA synthesis and the binding of actinomycin to the chromatin, implying alterations in the DNA template availability for transcription. The authors propose that the observed increased binding sites for actinomycin D are indicative of active chromatin configuration changes associated with lymphocyte activation.

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References (14)

  1. REFERENCES
  2. Bender, M A & Prescott, D M, Exptl cell res 27 (1962) 221. Black. M M & Anslev. H R. J cell bio126 (1965) 201.
  3. Bonn&, J & Huang, R C, J mol biol6 (1963) 169.
  4. Burton, D W, Exptl cell res 49 (1968) 300.
  5. Cooper, H & Rubin, A, Blood 25 (1965) 1014.
  6. Darivnkiewicz, Z. Krassowski. T & Skoninska. E, Nature 207 (1965) 14.02. . - _.
  7. Goldberg, I H, Reich, E & Rabinowitz, M, Nature 199 (1963) 44.
  8. Killander, D & Rigler, R, Exptl cell res 39 (1965) 701. -Ibid. 54 (1969) 163.
  9. Kay, J & Korner, A, Biochem j 100 (1966) 815.
  10. Nowell, P, Cancer res 20 (1960) 462.
  11. Pogo, B G T, Allfrey, V G & Mirsky, A E, Proc natl acad sci US 55 (1966) 805.
  12. Reich. E & Goldberg. I H, Progress in nucleic acid research and molecular biology 3 (1964) 183.
  13. Rinaertz. N R & Bolund. L, Biochim biovhvs acta 17431969) 147. , _ - Ringertz, N R, Dariynkiewicz, Z & Bolund, L, Exptl cell res. In press.
  14. Zetterberg, A & Auer, G. To be published. Received December 9, 1968