22-Malo et al 2010-Adaptation to captivity inbreeding and sperm (original) (raw)
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Biology of Reproduction, 2010
Mice (Peromyscus leucopus noveboracensis) from a captivebreeding program were used to test the effects of three genetic breeding protocols (minimizing mean kinship [MK], random breeding, and selection for docility [DOC]) and inbreeding levels on sperm traits and fertility. Earlier, in generation 8, one DOC replicate went extinct because of poor reproductive success. By generation 10, spermatozoa from DOC mice had more acrosome and midpiece abnormalities, which were shown to be strong determinants of fertility, as well as lower sperm production and resistance to osmotic stress. In addition, determinants of fertility, including male and female components, were assessed in a comprehensive manner. Results showed that the probability (P) of siring litters is determined by sperm number, sperm viability, and midpiece and acrosome abnormalities; that the P of siring one versus two litters is determined by tail abnormalities; and that the total number of offspring is influenced by female size and proportion of normal sperm, showing the relative importance of different sperm traits on fertility. On average, males with 20% normal sperm sired one pup per litter, and males with 70% normal sperm sired eight pups per litter. Interestingly, the proportion of normal sperm was affected by docility but not by relatively low inbreeding. However, inbreeding depression in sperm motility was detected. In the MK group, inbreeding depression not only affected sperm motility but also fertility: An increase in the coefficient of inbreeding (f ) of 0.03 reduced sperm motility by 30% and translated into an offspring reduction of three pups in second litters. A genetic load of 48 fecundity equivalents was calculated.
Behavioural risk factors in the reproduction of inbred and outbred oldfield mice
Animal Behaviour, 1997
The present study investigated two rarely measured aspects of inbreeding depression: the relationship between inbreeding and behaviour, and the possibility that inbred individuals that survive infancy may still suffer from inbreeding depression by failing to breed or failing to show appropriate mating or parental behaviours. Specifically, the relationship between (1) behaviour at pairing and reproductive success, (2) inbreeding and reproductive success and (3) inbreeding and pairing behaviour, was examined in two subspecies of the oldfield mouse, Peromyscus polionotus. Effects of parental and offspring inbreeding were separated through experimental design and analysis. Activity level during the first 25 days after pairing predicted future reproductive success: pairs that remained less active during the nocturnal (active) period were significantly less likely to breed than pairs that remained more active. Inbred females took significantly longer to produce their first litters and were less likely to produce litters than were outbred females, independently of whether females were related to their mates (i.e. whether their offspring would be inbred). Inactive pairs averaged fewer surviving pups than did active pairs. Inbreeding coefficient of female was a significant predictor of activity level in one of the two subspecies, suggesting that inbreeding may affect behaviour. Inbred adult females showed inbreeding depression in the form of lower conception rates and fewer surviving offspring, although the specific traits affected differed for the two subspecies. The implications for captive breeding programs, and likely causes of the subspecific differences, are discussed.
Proceedings of the Royal Society B: Biological Sciences, 2000
We studied three closely related species of endangered gazelles (Gazella dorcas, Gazella dama and Gazella cuvieri) with di¡erent levels of inbreeding in order to determine at which intensities inbreeding in£uences ejaculate traits. We also examined whether £uctuating asymmetry (FA) is a reliable indicator of genetic as well as reproductive stress. Our results show that, within each population, the individual coe¤cient of inbreeding is inversely related to ejaculate quality only in the species with the highest levels of inbreeding (G. cuvieri). In addition, FA is a reliable indicator of individual levels of inbreeding in both the species with the highest levels of inbreeding (G. cuvieri) and the species with intermediate levels of inbreeding (G. dama).
Heterozygosity-Fitness Correlations and Inbreeding Depression in Two Critically Endangered Mammals
Conservation Biology, 2012
The relation among inbreeding, heterozygosity, and fitness has been studied primarily among outbred populations, and little is known about these phenomena in endangered populations. Most researchers conclude that the relation between coefficient of inbreeding estimated from pedigrees and fitness traits (inbreeding-fitness correlations) better reflects inbreeding depression than the relation between marker heterozygosity and fitness traits (heterozygosity-fitness correlations). However, it has been suggested recently that heterozygosity-fitness correlations should only be expected when inbreeding generates extensive identity disequilibrium (correlations in heterozygosity and homozygosity across loci throughout the genome). We tested this hypothesis in Mohor gazelle ( Gazella dama mhorr) and Iberian lynx ( Lynx pardinus). For Mohor gazelle, we calculated the inbreeding coefficient and measured heterozygosity at 17 microsatellite loci. For Iberian lynx, we measured heterozygosity at 36 microsatellite loci. In both species we estimated semen quality, a phenotypic trait directly related to fitness that is controlled by many loci and is affected by inbreeding depression. Both species showed evidence of extensive identity disequilibrium, and in both species heterozygosity was associated with semen quality. In the Iberian lynx the low proportion of normal sperm associated with low levels of heterozygosity was so extreme that it is likely to limit the fertility of males. In Mohor gazelle, although heterozygosity was associated with semen quality, inbreeding coefficient was not. This result suggests that when coefficient of inbreeding is calculated on the basis of a genealogy that begins after a long history of inbreeding, the coefficient of inbreeding fails to capture previous demographic information because it is a poor estimator of accumulated individual inbreeding. We conclude that among highly endangered species with extensive identity disequilibrium, examination of heterozygosity-fitness correlations may be an effective way to detect inbreeding depression, whereas inbreeding-fitness correlations may be poor indicators of inbreeding depression if the pedigree does not accurately reflect the history of inbreeding.
Biology of Reproduction, 2010
Inbreeding is known to cause deleterious effects upon reproduction and survival, but its effects upon sperm DNA integrity have not been examined. In the present study, we analyzed this relationship among three endangered ungulates: Gazella cuvieri, Gazella dama mhorr, and Gazella dorcas neglecta. In addition, we examined whether levels of sperm DNA fragmentation are associated with semen quality. The magnitude of sperm DNA damage in the two species with high levels of inbreeding (G. cuvieri and G. dama mhorr) was extremely high when compared to the species with low levels of inbreeding (G. dorcas neglecta) and to values previously reported for outbred populations. Levels of sperm DNA fragmentation significantly increased with inbreeding and age. Increased DNA damage in sperm was associated with increased sperm head abnormalities, lower percentage of sperm with an intact acrosome, and poor motility. Our findings suggest that the link between inbreeding and semen quality is mediated by the effects of inbreeding upon sperm DNA damage. The deleterious effects of inbreeding upon the paternal genome likely decrease male fertility and may cause genetic damage to future generations. Because inbreeding is common among endangered species, high levels of sperm DNA damage may have considerable impact upon the viability of their populations.
Genetic evidence of promiscuity in Peromyscus leucopus
Behavioral Ecology and Sociobiology, 1991
We collected pregnant female Peromyscus leucopus from natural populations during the summer of 1987 and 1988 and allowed these females to give birth to their field-conceived young in the laboratory. Blood samples were taken by suborbital puncture and phenotypes of five genetic loci (Esterase-l, trasferrin, hemoglobin, albumin and 6-phosphogluconate dehydrogenase) were studied using horizontal starch-gel electrophoresis to detect multiple paternity in single litters. Only esterase-1 was found to be highly polymorphic, with four alleles in samples of both years. One litter out of 29 in 1987 and 6 litters out of 32 in 1988 contained three different paternal alleles and served as direct evidence of multiple paternity in the field. The proportion of females engaging in multiple matings in natural populations of P. leucopus, assuming that all males were involved in every multiple mating, is 25%-100% (mean 68%). Because it is unlikely that all males are involved in every multiple mating, the actual proportion of females engaging in multiple matings should be greater.
Effect of inbreeding depression on bull sperm quality and field fertility
Reproduction, fertility, and development, 2015
The present study investigated the effect of inbreeding depression on sperm quality using automated and objective methods and subsequent effects on beef bull field fertility. Individual inbreeding coefficient (F) values and field fertility data were determined using a dataset of AI bulls belonging to the Spanish Retinta Breeders Association (Asociación Nacional de Criadores de Ganado Vacuno Selecto de Raza Retinta (ANCRE)). Animals were clustered in two groups according to the F values as follows: (1) a high inbreeding group (HI; F ≥ 13.5%, mean 16.3); and (2) a non-inbreeding group (NI; F = 0%). In total, 17 different assessments were performed in both experimental groups, including evaluation of sperm morphology, acrosomal and DNA status, sperm plasma membrane integrity and function (hypo-osmotic swelling test), 10 kinetic parameters and the structure of sperm subpopulations. Sperm morphology, acrosomal and DNA status and osmotic tolerance were similar in both groups. Three veloci...