Inhibitory effects on the DNA binding of AP-1 transcription factor to an AP-1 binding site modified by benzo[α]pyrene 7,8-dihydrodiol 9,10-epoxide diastereomers (original) (raw)

To whom reprint requests should be addressed Benzo[a]pyrene 7,8-dihydrodiol 9,10-epoxide is an established carcinogen, known to covalently bind to DNA, in particular to the exocyclic aminogroup of dG, and thereby cause conformational changes to the double helix. AP-1 is a well-studied transcription factor that specifically binds to the DNA sequence S'-d(TGAGTCA). The effects of more or less randomly distributed BPDE adducts on DNA have been studied in different contexts, as well as the effects of different stimuli on transcription factor binding affinity and expression, but so far no investigation has been made concerning the effect of specific modification of a transcription factor binding site.