Análise histológica do Bio-Oss e Biogran em tíbia de ratos (original) (raw)
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Revista de Odontologia da UNESP, 2015
OBJETIVO: Avaliar histomorfometricamente o efeito de biovidro (B), osso bovino liofilizado (OB) ou da mistura desses dois biomateriais (B/OB - 1:1) no reparo de defeitos ósseos críticos em calvária de ratos. MATERIAL E MÉTODO: Defeitos ósseos (8 mm Ø) foram criados cirurgicamente na calvária de 24 ratos, distribuídos em 4 grupos com 6 animais, de acordo com o tipo de biomaterial: coágulo sanguíneo (GC), biovidro (GB), osso bovino liofilizado (GOB) e a mistura desses dois biomateriais (GB/OB). Os animais foram eutanasiados após 15 e 60 dias do procedimento cirúrgico (3 animais por período). A avaliação histológica foi baseada na descrição da morfologia dos tecidos neoformados, enquanto para a avaliação histomorfométrica foi realizada quantificação da porcentagem de tecido ósseo, de tecido conjuntivo fibroso neoformados e de biomaterial remanescente no defeito ósseo. RESULTADO: Nos dois períodos experimentais, a análise histológica apresentou neoformação óssea, principalmente nas bord...
Acta Ortopédica Brasileira, 2005
Foi realizado estudo experimental em ratos para avaliar o efeito do anticoagulante na consolidação óssea, conforme critérios clínicos, anatomopatológicos e biomecânicos. Manualmente, após perfuração do osso, foi produzida fratura aberta, na diáfise da tíbia direita, mantida sem imobilização, em 72 ratos machos da linhagem Wistar, com 60 dias de idade e peso médio de 242 gramas. Doze horas após a fratura, foi iniciado tratamento anticoagulante, mantido por 28 dias. Via subcutânea, um grupo recebeu heparina sódica na dose de 200UI/Kg de 12 em 12 horas, enquanto outro, recebeu enoxaparina na dose de 1mg/Kg de 12 em 12 horas, doses preconizadas para tratamento do tromboembolismo em humanos. O terceiro grupo, controle, recebeu água destilada. Durante o experimento, os animais foram avaliados clinicamente e após 28 dias, sacrificados. Nos animais dos três grupos, a evolução clínica foi semelhante. Mediante análise anatomopatológica efetuada por estudo descritivo e quantitativo, foi observ...
Avaliação quantitativa em cortes histológicos transversais do couro cabeludo
Anais Brasileiros de Dermatologia, 2006
FUNDAMENTOS: A biópsia por punch é de grande auxílio no diagnóstico das doenças do couro cabeludo quando avaliada por patologista experiente. O conhecimento das estruturas encontradas no couro cabeludo normal facilita o diagnóstico histopatológico nessa área tão complexa da dermatopatologia. OBJETIVO: Determinar dados quantitativos normais do couro cabeludo de adultos brancos. MÉTODOS: Em 20 necrópsias, 80 fragmentos de couro cabeludo clinicamente normal foram obtidos por punch 4mm. As amostras foram avaliadas em cortes transversais. RESULTADOS: Em fragmentos obtidos de adultos brancos, com idade variando entre 21 e 78 anos, foi possível observar (medianas): 16,5 (12-23) folículos totais; oito (6-9) unidades foliculares; 15 (10-20) folículos terminais; um (0-2) folículo velo; um (0-2,5) trato fibroso; 92,2% de folículos anágenos; 1,6% de catágenos e 6,2% de telógenos. CONCLUSÃO: Além dos resultados apresentados, observaram-se ainda nos indivíduos do sexo masculino: menor número de u...
Int. J. Morphol
This study assessed the micro anatomical differences in the tongue of rat, bat and pangolin with a view to establishing the functional anatomical differences of these mammalian tongues on their dietary pattern. Ten rats, ten bats and ten pangolins were used for this study. The animals were sacrificed and the tongue excised and processed for light microscopical study adopting the following stains: Haematoxylin Eosin, Verhoeff Gieson and Masson trichrome. The results showed non papillation of the keratinized stratified epithelium of pangolin tongue unlike the papillation seen in the tongue of the rat and bat. While the filiform papillation seen in the rat was bristle like, the filiform papillae in the bat were crown-like. There was also an unusual dense collagenous ring in the proximal portion of the pangolin tongue which was absent in other mammals. There were taste buds along the lateral walls of the vallate papillae in the distal portion of the tongue of rats and bats but none was found in the pangolins. In conclusion, the morphology of the tongues of these mammals showed a relationship between their feeding pattern and the adaptive changes in the microanatomy of their tongue.
Veterinarski Arhiv, 2020
RooZBeh, B., M. MoAZZAMi, A. RAshidlAMiR, Z. MoosAvi, A. JAvAdMAnesh: The effects of short term growth hormone administration and resistance training on the histopathology and mutation of the BRAF gene (T1799A) in thyroid tissue of the brown rat (Rattus norvegicus). vet. arhiv 90, 403-412, 2020. ABsTRACT High levels of growth hormone accelerate mitosis rate but decrease the apoptosis process in its target organs. These events might cause the initiation of different cancer types. Thus, the main aims of this study were assessing the effects of short term growth hormone administration and resistance training on the histopathology and detection of the BRAF-V600E mutation in the thyroid tissue of male Rattus norvegicus brown rats. Thirty-two rats were randomly divided into four groups. After 8 weeks of the experiment (i.m), thyroid tissue and blood samples of saline (CS), resistance training+saline (RS), growth hormone (2 mg/kg) (GI) and resistance training+growth hormone (2 mg/kg) (RG) were taken to evaluate histopathology, the BRAF T1799A mutation of thyroid tissue, and circulating levels of IGF-1 and IGFBP-3. The protocol of training consisted of rats climbing a ladder while carrying weights (3 sets/5 reps). Microscopic evaluation of thyroid tissue did not show any histopathological changes, and there were no mutations in the studied region of the BRAF sequence. Serum IGF-1 concentration was significantly lower in the RS group than in other groups (P<0.05). However, serum IGFBP-3 concentration did not change significantly in the RS group. Moreover, serum IGF-1 and IGFBP-3 concentrations were significantly higher in the GI and RG groups than in the others (P<0.05). In conclusion, the decrement of serum IGF-1 concentration and IGF-1/IGFBP-3 ratio after resistance training might decrease the risk of cancer. Furthermore, short term growth hormone administration, with and without resistance training, might increase the risk of cancer through the high levels of serum IGF-1 concentration and IGF-1/ IGFBP-3 ratio in male rats.
In this study, the superior colliculus (SC) of rat, bat and pangolin were compared using histological and quantitative histochemical parameters to observe possible modification that enable these mammals to cope with their habitation particularly with respect to their diet. The study was conducted using ten adult Wistar rats, ten fruit bats and eight pangolins comprising of both sexes. After being sacrificed by cervical dislocation, their skulls were opened using bone forceps to expose the brains. The superior colliculi were excised from each brain, homogenized and read spectrophotometrically for the activities of lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G-6-PDH), acid phosphatase (ACP) and alkaline phosphatase (ALP). The SC tissue sample meant for histological studies were fixed in 10% formol calcium and processed for paraffin wax embedding. Serial sections of 3μm thickness were stained with Hematoxylin and Eosin and Cresyl fast violet stains. The stained tissues were studied under the light microscope. Application of one-way ANOVA statistical analytical method showed that there were significant differences (p<0.05) in the activities of LDH, G-6-PDH, ACP and ALP of the SC of the three mammals as revealed in the quantitative histochemistry of these enzymes and markers. Histological observations revealed variations in the distribution of neurons and their supporting glial cells with the neurons in the SC of the rat appearing more numerous as well as in the pangolin and are fewer in the bat. The comparison of the differences observed in the histological and the quantitative histochemical activities in these mammalian species revealed a variation in the visual ability and their individual peculiarities in relation to their mode and pattern of living.
Aspectos histológicos e ultraestruturais da língua em ratos desnutridos
Revista Brasileira de Otorrinolaringologia, 2006
Poucos estudos sobre os efeitos da desnutrição protéico-calórica na mucosa oral são encontrados na literatura. OBJETIVOS: O objetivo deste trabalho é verificar os efeitos da desnutrição protéico-calórica na mucosa da língua de ratos adultos, envolvendo microscopia óptica e eletrônica de varredura. MATERIAIS E MÉTODOS: Um estudo experimental foi realizado em trinta ratos Wistar, 15 controles e 15 com desnutrição protéico-calórica. O último grupo recebeu ração em pequena quantidade, com menor conteúdo de caseína, durante 45 dias. Os ratos foram pesados a cada 3 dias, do primeiro (90 dias de vida) ao último dia (45 dias de dieta), quando foram sacrificados. Foi realizada eletroforese de proteínas plasmáticas e as línguas foram preparadas para microscopia óptica comum e eletrônica. Para análise estatística utilizou-se análise de variância e teste T de Student. RESULTADOS: Foi observada uma significante diminuição no peso e nas proteínas plasmáticas dos ratos com desnutrição protéico-cal...
Compared Anatomy and Histology between Mus musculus Mice (Swiss) and Rattus norvegicus Rats (Wistar)
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The evolution of knowledge in biological and medical areas which made possible scientific and technological advances are attributed to anatomical studies, physiology and immunology in animals, contributing to the discovery of prophylactic measures and treatments of diseases that affect humans and animals. Currently, substitution is suggested by alternative methods that do not use laboratory animals, however in vitro methods may never be able to provide similar results to in vivo methods. Mice and rats are the most used animals in experimental researches, the anatomical, histological and genetic differences between species should be carefully evaluated, to better apply the study model and avoid unnecessary waste avoid. This study, aimed at an anatomical comparative and histological relationship between a rat´s and a mouse´s organs, is of great importance in experimental studies. For such, 30 Mus musculus (Swiss) mice and 15 Rattus norvegicus (Wistar) male and heterogenic rats, were u...
Estudo histológico dos enxertos ósseos homólogos humanos
Acta Ortopedica Brasileira, 2003
Due to the increasing use of the human allografts in the reconstructive orthopedic surgeries, the complete knowledge of its biomechanics and hystologics characteristic is needed. This study compared allografs picked of femoral condilar from cool air cadavers and after cryopreservation (under -80°C) for thirty days with relationship to the hystologic´s characteristic.