Plant regeneration from root protoplasts of Brassica (original) (raw)

1982, Plant Science Letters

Protoplasts isolated enzymatically from roots of Brassica alba (White Mustard), B. campestris {Turnip), B. napus (Rape) and B. oleracea (Cabbage), divided to form callus. Plant regeneration was obtained from protoplast derived tissues of B. napus and B. oleracea, but only rhizogenesis was observed with B. campestris. Tissues of B. alba remained undifferentiated. The suitability of root protoplasts for genetic manipulations in the genus Brassica is discussed. *On leave from the Institute of Plant Physiology, Academia Sinica, Shanghai, China. Abbreviations: BAP, 6-benzylaminopurine; CPA, p-chlorophenoxyacetic acid; IAA, 3-indoleacetic acid; NAA, a-naphthaleneacetic acid; 2,4-D, 2,4-dichlorophenoxyacetic acid. 0304--4211/82/0000--0000/$02.75

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