DHM (Digital Holography Microscope) for imaging cells (original) (raw)
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Digital Holographic Microscopy (DHM)
Imaging & Microscopy, 2006
Digital Holographic Microscopes (DHM) enables strictly noninvasive visualisation of unstained transparent and partially reflective specimens, in real time, by providing simultaneously amplitude and phase changes of a light wave transmitted or reflected. They are used for characterisation of samples at the nanometer scale, for quality control on production line, and for dynamical analysis of biological specimen and micro systems. more than 15 reconstructions per second for 512 x 512 pixels holograms with a standard personal computer.
Digital holographic microscopy applied to life sciences
2007 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2007
Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of erythrocyte cells. DHM has the advantage to be non-invasive (no phototoxicity, no contrast agents) and allows a high throughput measurements.
Exploring Neural Cell Dynamics with Digital Holographic Microscopy
Digital Holography and Three-Dimensional Imaging, 2013
Quantitative phase signal (QPS) of digital holography microscopy has permitted to investigate Cellular Membrane nano-Fluctuations (CMnF), of red blood cells as well as to non-invasively provide an optical signature of the electrical activity of cells.
11 Digital Holography and Cell Studies
2011
Digital holographic microscopy (DHM) is a novel high-resolution imaging technique that offers real-time imaging and quantitative measurements of physiological parameters. It has developed into a broad field, and one of many interesting applications is to study cells without staining or labeling them and without affecting them in any way. Digital holography makes it possible to easily measure cell properties that previously have been very difficult to study in living cells, such as cell thickness, volume, and cell refractive index (Marquet et al., 2005; Rappaz et al. 2005; Mölder et al., 2008; El-Schish et al., in press; Persson et al., in press). Living, dying or dead cells as well as fixed cells can be studied. The first DHM images showing living cells were published in 2003 and 2004 (You et al., 2003; Carl et al., 2004), making this field of research rather new. Two of the most interesting functions of DHM is 3-D imaging of objects and to make in-focus measurements over time. Digi...
Biophotonics and New Therapy Frontiers, 2006
We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to λ/1800, and the low acquisition time (down to 20 µs) enables to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the intracellular refractive index and the cellular thickness (morphometry) from the measured phase shift is presented.
Digital holographic microscopy for study cellular dynamics
Novel Optical Instrumentation for Biomedical Applications II, 2005
Based on an original numerical reconstruction algorithm (E. Cuche et al. Appl. Opt. 38, 6994 1999), we have developed a Digital Holographic Microscope (DHM), in a transmission mode, allowing to investigate cellular structures and dynamics.
Multicolor digital holographic microscope (DHM) for biological purposes
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 2010
Our digital holographic (DH) approach can be used to study tissue structures both in vitro and in vivo. This DHM architecture can produce three color microscopic 3D and 4D (video) images. We record 3 color (RGB) holograms with single exposures, and the perfect compensation of color crosstalk is solved. An in-line holographic setup and reconstruction algorithms are presented with demonstrative simulations and experimentally captured and numerically reconstructed images. Comparing the individually reconstructed color images with each other can provide information both for recognition of different types of cells or microorganisms, and for diagnostic purposes as well. Experimental example is given observing microscopic hydro-biological organisms using a color digital holographic microscope.
Use of digital holographic microscopy in tomography
Biophotonics and New Therapy Frontiers, 2006
Digital Holographic Microscopy (DHM) provides three-dimensional (3D) images with a high vertical accuracy in the nanometer range and a diffracted limited transverse resolution. This paper focuses on 3 different tomographic applications based on DHM. First, we show that DHM can be combined with time gating: a series of holograms is acquired at different depths by varying the reference path length, providing after reconstruction images of slices at different depths in the specimen thanks to the short coherence length of the light source. Studies on enucleated porcine eyes will be presented. Secondly, we present a tomography based on the addition of several reconstructed wavefronts measured with DHM at different wavelengths. Each wavefront phase is individually adjusted to be equal in a given plane of interest, resulting in a constructive addition of complex waves in the selected plane and destructive addition in the others. Varying the plane of interest enables the scan of the object in depth. Thirdly, DHM is applied to perform optical diffraction tomography of a pollen grain: transmission phase images are acquired for different orientations of the rotating sample, then the 3D refractive index spatial distribution is computed by inverse radon transform. The presented works will exemplify the versatility of DHM, but above all its capability of providing quantitative tomographic data of biological specimen in a quick, reliable and non-invasive way.