555 Identification and characterization of IgE binding epitopes of patatin, a major food allergen of potato (original) (raw)

This study investigates the IgE binding epitopes of patatin, a significant allergen found in potatoes. Through the synthesis of overlapping oligopeptides and immunoblotting techniques, the research identifies the 42 kDa patatin protein as a key allergen among five patients with confirmed potato allergies. It highlights the potential for anaphylaxis due to macadamia nut ingestion and characterizes the stability and immunogenicity of allergens such as patatin and Ara h 2 from macadamia nut, showing their resistance to enzymatic digestion.