Dispersed repetitive elements in mouse genome analysis (original) (raw)
Related papers
A family of moderately repetitive sequences in mouse DNA
Nucleic Acids Research, 1980
When mouse DNA is digested to completion with restriction endonuclease Eco Rl, a distinct band of 1.3 kb segments comprising about 0.5-3% of thegenomeis observed upon agarose gel electrophoresis. This DNA is not tandemlyrepeated in the genome and is not derived from mouse satellite DNA. Restriction endonuclease analysis suggested that the 1.3 kb segments are heterogeneous. Specific sequences were selected from the 1.3 kb segments and amplified by cloning in plasmid pBR322. Southern transfer experiments indicated that three separately cloned mouse DNA inserts hybridized predominantly to the Eco R1 1.3 kbband and to the conspicuous subsegments generated by secondary restriction endonuclease cleavage of the sucrose gradient purified 1.3 kb segments. Segments were also excised by Hha I (Hha I segments) from the chimeric plasmids containing mouse DNA inserts and subjected to restriction endonuclease and cross-hybridization analysis. It was found that the three Hha I segments were different, although two of them exhibited partial sequence homology. Cot analysis indicated that each of the Hha I segments are repeated about 104 times in the mouse genome. These findings indicate that a family of related but non-identical, moderately repetitive DNA sequences, rather than a single homogeneous repeat, is present in the 1.3 kb Eco RI band.
Studies of a novel repetitive sequence family in the genome of mice
European Journal of Biochemistry, 1988
A new middle repetitive sequence is described in the mouse genome. It has been revealed with a recombinant clone isolated from a Mus musculus BamHI gene library constructed in pBR322 and containing an insertion of 1.73 kb. When digests of genomic DNA were subjected to Southern blot hybridization, using the 1.73-kb insert as probe, we obtained a light smear and discrete bands, indicating a dispersion in the mouse genome of this sequence. This 1.73-kb sequence seems to be a part of a greater repetitive sequence at least 6 kb in length.
DNA repetitive sequences-types, distribution and function: A review
The development and use of molecular markers for the detection and exploitation of DNA polymorphism is one of the most significant developments in the field of molecular genetics. DNA based molecular markers have acted as versatile tools and have found their own position in various fields like taxonomy, physiology, embryology, genetic engineering etc. A major step forward in genetic identification is the discovery that about 30-90% of the genome is constituted by regions of repetitive DNA which are highly polymorphic in nature. Microsatellites are multilocus probes creating complex banding patterns and are usually non-species specific occurring ubiquitously. They form an ideal marker system and are dominant fingerprinting markers and co-dominant STMS (sequence tagged microsatellites) markers. Microsatellites markers have been used successfully to determine the degree of relatedness among individuals or groups of accessions to clarify the genetic structure or partitioning of variation among individuals, accessions, populations and species. Repetitive sequences have been widely used for examining genome and species relationships by in situ and by Southern hybridization.
Proceedings of The National Academy of Sciences, 1982
We have investigated the organization and the distribution of a family of interspersed DNA repeats in the mouse genome. The repeats are at least 5600 base pairs (bp) in size and contain two contiguous BamHI endonuclease fragments, 4000 and 540 bp in size, the larger of which includes a 1350-bp EcoRI fragment studied by previous authors. The repeats are polymorphic in their restriction maps, and represent the major family of interspersed repeats in the mouse genome. The repeats are present almost exclusively in the two light major components of mouse DNA, and the base composition of their large BamHI fragments matches that ofthose components. The genomic distribution ofthe repeats is different from that of structural genes, which are present not only in the two light components but also in the two heavy components of mouse DNA. This distribution indicates that the repeats are not involved, at least in any simple way, in the regulation of gene expression.
A cluster of repetitive elements within a 700 base pair region in the mouse genome
Nucleic Acids Research, 1983
Approximately 39% of the dones from a BALB/c mouse genomic library hybridized with polyadenylated cytoplasmic RNA extracted from anemic mouse spleen. The DNA sequence of a portion of one such clone revealed the presence of three repetitive sequence elements within a 700 bp span. All three elements contain putative RNA polymerase III control regions oriented in the same direction and oligo(dA) tracts at their 3' ends. The first element is a member of the murine Bl family. A comparison of this element with other Bl family members indicates that the Bl family can be divided Into two subclasses based on commonly held base changes and deletions. The second element within this 700 bp region may be a member of a new murine Alu family. Its structure is analogous to other murine Alu-equivalent sequences with respect to overall length, the presence of a 3' oligo(dA) tract and putative RNA pdymerase HI control regions. The third element is a murine type 2 Alu-equivalent sequence.
Prototypic sequences for human repetitive DNA
Journal of Molecular Evolution, 1992
We report a collection of 53 prototypic sequences representing known families of repetitive elements from the human genome. The prototypic sequences are either consensus sequences or selected examples of repetitive sequences. The collection includes: prototypes for high and medium reiteration frequency interspersed repeats, long terminal repeats of endogenous retroviruses, alphoid repeats, telomere-associated repeats, and some miscellaneous repeats. The collection is annotated and available electronically.[/ap ]
A ubiquitous family of repeated DNA sequences in the human genome
Journal of Molecular Biology, 1979
Renatured DNA from hnman and rnarly othrr cukaryotes is known to contairl 300-nncleotide duplex regions formed from rellatnred rrpcatcd sequences. These short repeated DNA sequences are widely believed to hr interspersed with single copy DNA sequences. In this work we show that at least half of these 300-n~cleotide duplexes share a cleavapt: site for t,ho restriction enz.yme AluI. This site is lonatrd I70 nncleotides from one end. This 41~ family of repeated sequences makes up at least 3% of the genome and is present ill sovrral hnndrod thollsand topics. lnvcrtrd repeated sequences are also known to contain a short 300-nucleotidv duplex region. We find that at least half of ttre 3OO~Illlcle:ot,ide duplex regions irl iIlvcrtt,d repeated sequences also have an al~l wstrictiorl site located 170 II~IC~~~Otides from one end. By driven rcnaturation trcllniqncs, tllc Alec family is shown to be distributed over a minimum range of 307/, to 607; of the penomc. (Tile breadth of this ralrge wflects tllo presence of inverted repeated sequences wllich, in part, inclrlde t,lw ,417~ family.) These findings imply that tile interspersion pattern of repeated and single copy sequences in human DNA is larpely dominated by one family of repeatcd sequences.