The potential dolichol recognition sequence of β -1,4-mannosyltransferase is not required for enzymic activity using phytanyl-pyrophosphoryl-α- N,N '- diacetylchitobioside as acceptor (original) (raw)

The study investigates the importance of the potential dolichol recognition sequence (PDRS) in the enzymatic activity of β-1,4-mannosyltransferase using a phytanyl analogue as an acceptor substrate. Findings indicate that the PDRS is not essential for the enzyme's activity, as similar apparent Km values and diastereoselectivity were maintained without it. The research highlights differences in the role of PDRS across various glycosyltransferases and demonstrates successful bacterial expression of the enzyme in a soluble form.