Lasiodiplodia gonubiensis sp. nov., a new Botryosphaeria anamorph from native Syzygium cordatum in South Africa (original) (raw)
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2016
Abstract: Botryosphaeria spp. are common and widely distributed pathogens on many economically important crops, includ-ing forest tree species. These fungi cause a wide variety of symptoms on trees of all ages, but are mostly associated with canker and die-back of branches and main stems. As disease agents, Botryosphaeria spp. are often encountered in their anamorph state, namely species of Fusicoccum, Diplodia or Lasiodiplodia. During a recent survey of botryosphaeriaceous fungi from native Syzygium cordatum in South Africa, an unfamiliar Lasiodiplodia sp. was isolated. The aim of this study was to compare this apparently undescribed species with other species of Botryosphaeria using morphological characteristics and DNA sequence data of the rDNA internal transcribed spacers, ITS1 and ITS2. Based on sequence data, the isolates from S. cordatum were more closely related to B. rhodina (anamorph Lasiodiplodia theobromae) than to other Botryosphaeria spp., but also phylogenetically dis...
Phylogeny and morphology of Lasiodiplodia species associated with Magnolia forest plants
Scientific Reports
Two new species of Lasiodiplodia (Lasiodiplodia endophytica and Lasiodiplodia magnoliae) are described and illustrated from Magnolia forests in Yunnan, China. Endophytic and saprobic Lasiodiplodia pseudotheobromae and endophytic L. thailandica are new records from this host. The internal transcribed spacers (ITS), part of the translation elongation factor-1α (tef1) and partial β-tubulin (tub2) sequence data were analyzed to investigate the phylogenetic relationships of the new species with other Lasiodiplodia species. Lasiodiplodia magnoliae is phylogenetically sister to L. mahajangana and L. pandanicola but morphologically distinct from L. mahajangana in having larger conidia. Lasiodiplodia endophytica is most closely related to L. iraniensis and L. thailandica and the three species can be distinguished from one another by 2 base pair differences in ITS and three or four base pair differences in tef1. The new collections suggest that Magnolia forest plants are good hosts for Lasiod...
Journal of Fungi
Lasiodiplodia (family Botryosphaeriaceae) is a widely distributed fungal genus that causes a variety of diseases in tropical and subtropical regions. During 2020–2021, a routine survey of fruit tree plants was conducted in five Egyptian Governorates, and fresh samples exhibiting dieback, decline, leaf spot and root rot symptoms were collected. Collection from eight different symptomatic leaves, twigs, branches and roots of fruit trees yielded 18 Lasiodiplodia-like isolates. The sequencing data from the internal transcribed spacer region (ITS), partial translation elongation factor 1-alpha (tef1-a) and β-tubulin (tub2) were used to infer phylogenetic relationships with known Lasiodiplodia species. Two isolates obtained from black necrotic lesions on Phoenix dactylifera leaves were identified as a putative novel species, L. newvalleyensis sp. nov., and were thus subjected to further morphological characterization. The results of isolation and molecular characterization revealed that L...
Life, 2021
Botryosphaeriaceous fungi cause stem blight, canker and dieback in woody plants. During a survey on the fungal pathogens associated with blueberries in China, 135 blighted, cankered or dead blueberry branches were collected from Fujian and Shandong Provinces. Based on the morphological characterization and phylogenetic analyses of a concatenated ITS rDNA, tef1-α, TUB, and RPB2 loci, five new species of Lasiodiplodia, viz., L. clavispora, L. fujianensis, L. henanica, L. nanpingensis and L. paraphysoides were recognized. Detailed descriptions and illustrations, as well as multigene phylogenies, are provided in this paper. The diversity of plant pathogens on agriculturally and economically important plants is higher than anticipated.
Although several Botryosphaeriaceae species have been relatively well-studied on economically important crops and forest plantations, little is known regarding their presence and ecology on native tree species in the natural tropical forests in South America. Therefore, the aim of this study was to determine the fungi associated with blue stain symptoms of the wood on Ficus insipida in lumber yards from the Imataca natural forest in Eastern Venezuela using morphological descriptions and DNA sequences of the internal transcribed spacer region (ITS1-5.8S-ITS2) and part of the translation elongation factor 1-α (TEF) gene. Results of this study showed the botry-osphaeriaceous taxa Lasiodiplodia theobromae and L. venezuelensis to be the main fungi associated with blue stain symptoms. This study represents the first report of these fungi on F. insipida lumber in the Natural Forest of Venezuela.