The antibacterial properties of a novel chitosan–Ag-nanoparticle composite (original) (raw)
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Advances in Materials Science and Engineering, 2013
The aim of this study is to investigate the antibacterial properties and characterization of chitosan-silver nanoparticle composite materials. Chitosan-silver nanoparticle composite material was synthesized by adding AgNO3and NaOH solutions to chitosan solution at 95°C. Different concentrations (0,02 M, 0,04 M, and 0,06 M) of AgNO3were used for synthesis. Chitosan-silver nanoparticle composite materials were characterized by Transmission electron microscopy (TEM), X-ray diffraction (XRD), ultraviolet (UV) spectrophotometer, and Fourier transform infrared (FTIR) spectrometer techniques.Escherichia coli,Acinetobacter baumannii,Staphylococcus aureus,Enterococcus faecalis,Pseudomonas aeruginosa, andStreptococcus pneumoniaewere used to test the bactericidal efficiency of synthesized chitosan-Ag nanoparticle composite materials. The biological activity was determined by the minimum bacterial concentration (MBC) of the materials. Antibacterial effect of chitosan-silver nanoparticle materia...
Vietnam Journal of Science and Technology, 2018
A green and simple approach has been successfully developed to synthesize chitosan/Ag nanocomposites using kumquat extract as a biological reducing agent. It indicates to be an eco-friendly and green method for the synthesis providing a cost effective and an efficient route for the chitosan/Ag nanocomposites’ synthesis. The prepared chitosan/Ag nanocomposites have been characterized by UV-vis, TEM, FTIR, and XRD. Result showed those chitosan/Ag nanocomposites have been obtained with average particle size ~15-25 nm. Moreover, the synthesized chitosan/Ag nanocomposites also showed their efficient antimicrobial activity against S. aureus and E. coli. The chitosan/Ag nanocomposite was found to have significantly higher antimicrobial activity than its components at their respective concentrations. The presence of a small percentage (2.5 %, w/w) of metal nanoparticles in the nanocomposite was enough to significantly enhance inactivation of S. aureus and E. coli as compared with unaltered ch...
Due to their antibacterial activity and biocompatibility, chitosan and chitosan derivatives have ability of participating in biological applications. The prepared Cs g-PAN/Ag nanocomposites are reported as antibacterial agents that exhibit efficient antibacterial activity in vitro. The prepared chitosan-g- PAN/Ag nanocomposite was provided by FTIR and gravimetric methods. UV spectra and TEM images show silver nanoparticles with average 15 20 nm dispersed homogeneously in (CS-g-PAN/Ag) nanocomposite. The antimicrobial activity examined against gram negative bacterium (E. coli) and gram positive bacterium (Staphylococcus aureus) in addition to yeast (Candida albicans) and fungi (Aspergillusniger) is evaluated in vitro. The MIC for E. coli for in vivo application was also examined. In vivo antibacterial activity against E. coli has been evaluated by using an intestine-infected rat model. Experimental results indicated that the number of bacteria surviving in the small intestine is lower than in the untreated group. These nanocomposite open up a new avenue for design and synthesis of next-generation antibacterial agents as alternatives to antibiotics.
Scientific Journal for Damietta Faculty of Science, 2019
The present study provided a green approach for chitosan/silver nanocomposite (CS/AgNC) synthesis. CS/AgNC were synthesized by a biological method using the supernatant of Escherichia coli D8 (MF062579) strain in the presence of sun light. The absorbance spectra of particle solutions have been characterized by UV-Vis spectrophotometry, Fourier transform-infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM). The obtained CS/AgNC showed absorption peak at 446 nm with an average particle size of ~21-31 nm. Moreover, CS/AgNC showed a potent antimicrobial activity against Candida albicans, Staphylococcus aureus and E. coli.
Polymers
Biosynthesized metal nanoparticles, especially silver and gold nanoparticles, and their conjugates with biopolymers have immense potential in various fields of science due to their enormous applications, including biomedical applications. Polymeric nanoparticles are particles of small sizes from 1 nm to 1000 nm. Among different polymeric nanoparticles, chitosan-coated silver and gold nanoparticles have gained significant interest from researchers due to their various biomedical applications, such as anti-cancer, antibacterial, antiviral, antifungal, anti-inflammatory technologies, as well as targeted drug delivery, etc. Multidrug-resistant pathogenic bacteria have become a serious threat to public health day by day. Novel, effective, and safe antibacterial agents are required to control these multidrug-resistant pathogenic microorganisms. Chitosan-coated silver and gold nanoparticles could be effective and safe agents for controlling these pathogens. It is proven that both chitosan ...
2018
A simple and eco-friendly approach has been successfully developed for the preparation of chitosan nanocomposites films loaded with silver nanoparticles using kumquat extract as a biological reducing agent. The chitosan/silver nanocomposites (CTS/Ag NCPs) films were prepared from chitosan/silver nanocomposites solution and dried for 14 h at 70C in a vacuum oven with the pressure of 0.03 Mpa. The morphology and characterization of CTS/Ag NCPs films have been also determined by FTIR, XRD, and SEM. The UV-vis spectroscopy and TEM image indicated that synthesized chitosan/silver nanocomposites have spherical shape with their uniform dispersion and their average particle size of about 20-30 nm. The prepared CTS/Ag NCPs films showed their great antibacterial activity on Staphylococcus aureus (S. Aureus) and Escherichia coli (E. coli). Therefore, this eco-friendly method that would be used for the preparation of chitosan/Ag nanocomposites films could be competitive and alternative to the e...
Factors Affecting the Antibacterial Activity of Chitosan-Silver Nanocomposite
IET Nanobiotechnology, 2017
This study provides the optimum preparation parameters of chitosan-silver nanoparticles composite (CSNC) with promising antibacterial activity against the most common bacterial infections found on burn wounds. CSNC was synthesised by simple green chemical reduction method with different preparation factors. Chitosan was used to reduce silver nitrate and stabilise silver nanoparticles in the medium. For this reason, spectroscopic and microscopic techniques as, ultraviolet-visible Fourier transform infrared spectroscopy and transmission electron microscopy were used in the study of the molecular and morphological properties of the resultant composites. Furthermore, the composite was assessed in terms of Ag-ions release by AAS and its efficacy as antibacterial material. As a result, CSNC showed stronger antibacterial effect than its individual components (chitosan and silver nitrate solutions) towards Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa and Escherichia coli) bacteria. CSNC prepared in this study showed highest inhibition percentage of bacterial growth up to 96% at concentration of 220 μg/ml.
Silver-N-Carboxymethyl Chitosan Nanocomposites: Synthesis and its Antibacterial Activities
Journal of Bioterrorism & Biodefense, 2010
In this work, silver-N-carboxymethyl chitosan nanocomposites (Ag-N-CMC) were synthesized in the homogeneous state via the reduction of Ag + (using [Ag(NH 3) 2 ]OH instead of AgNO 3) by NaBH 4 in the presence of water-soluble N-carboxymethyl chitosan as a stabilizer. The resulting Ag-N-CMG was characterized by FTIR, TEM and UV-vis spectra. The results showed that the average particle size of silver nanoparticles was little affected by the concentration of Ag + added and was between 2 and 10nm. The characteristic surface plasmon resonance band of silver nanoparticles centered at about 398-410nm. In vitro antibacterial activities of Ag-N-CMC nanocomposites were evaluated against both gram-negative bacteria: Escherichia coli ATCC 25922 (Ec) and Pseudomonas aeruginosa VM201 (Pseu) and gram-positive bacteria: Staphylococcus aureus ATCC 1128 (Sta) and Bacillus cereus ATCC 9946 (Bc). The results shown that the Ag-N-CMC nanocomposites could inhibit the growth and multiplication of the tested bacteria.
European Polymer Journal
n the present work chitosan-silver (CS/Ag) nanocomposites, either in the form of nanoparticles (AgNP) or as ionic dendritic structures (Ag+), are synthesized by a simple and environmentally friendly in situ chemical reduction process. The antibacterial activity of the resulting nanocomposites in the form of films is studied against two bacteria, Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The relationship between electrical, structural and antibacterial properties of CS/AgNP and CS/Ag+ nanocomposites are studied by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction, and UV-Vis, impedance, infrared and X-ray photoelectron spectroscopies. The results demonstrate that in contrast to CS/Ag+ ion films, the CS/AgNP composites films (average particle size less than 10 nm) showed a significantly higher antibacterial potency. The collective action of AgNP and Ag+ ions facilitate the enhancement and synergetic antibacteria...
Synergistic antibacterial activity of chitosan–silver nanocomposites on Staphylococcus aureus
Nanotechnology, 2011
The approach of combining different mechanisms of antibacterial action by designing hybrid nanomaterials provides a new paradigm in the fight against resistant bacteria. Here, we present a new method for the synthesis of silver nanoparticles enveloped in the biopolymer chitosan. The method aims at the production of bionanocomposites with enhanced antibacterial properties. We find that chitosan and silver nanoparticles act synergistically against two strains of Gram-positive Staphylococcus aureus (S. aureus). As a result the bionanocomposites exhibit higher antibacterial activity than any component acting alone. The minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations of the chitosan-silver nanoparticles synthesized at 0 • C were found to be lower than those reported for other types of silver nanoparticles. Atomic force microscopy (AFM) revealed dramatic changes in morphology of S. aureus cells due to disruption of bacterial cell wall integrity after incubation with chitosan-silver nanoparticles. Finally, we demonstrate that silver nanoparticles can be used not only as antibacterial agents but also as excellent plasmonic substrates to identify bacteria and monitor the induced biochemical changes in the bacterial cell wall via surface enhanced Raman scattering (SERS) spectroscopy.